Cytokeratin 18 (KRT18) (NM_000224) Human Untagged Clone

CAT#: SC120062

KRT18 (untagged)-Human keratin 18 (KRT18), transcript variant 1


  "NM_000224" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol KRT18
Synonyms CK-18; CYK18; K18
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000224 edited
GAATTCGGCACGAGGGTCGTCCGCAAAGCCTGAGTCCTGTCCTTTCTCTCTCCCCGGACA
GCATGAGCTTCACCACTCGCTCCACCTTCTCCACCAACTACCGGTCCCTGGGCTCTGTCC
AGGCGCCCAGCTACGGCGCCCGGCCGGTCAGCAGCGCGGCCAGCGTCTATGCAGGCGCTG
GGGGCTCTGGTTCCCGGATCTCCGTGTCCCGCTCCACCAGCTTCAGGGGCGGCATGGGGT
CCGGGGGCCTGGCCACCGGGATAGCCGGGGGTCTGGCAGGAATGGGAGGCATCCAGAACG
AGAAGGAGACCATGCAAAGCCTGAACGACCGCCTGGCCTCTTACCTGGACAGAGTGAGGA
GCCTGGAGACCGAGAACCGGAGGCTGGAGAGCAAAATCCGGGAGCACTTGGAGAAGAAGG
GACCCCAGGTCAGAGACTGGAGCCATTACTTCAAGATCATCGAGGACCTGAGGGCTCAGA
TCTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATGCCCGTCTTG
CTGCTGATGACTTTAGAGTCAAGTATGAGACAGAGCTGGCCATGCGCCAGTCTGTGGAGA
ACGACATCCATGGGCTCCGCAAGGTCATTGATGACACCAATATCACACGACTGCAGCTGG
AGACAGAGATCGAGGCTCTCAAGGAGGAGCTGCTCTTCATGAAGAAGAACCACGAAGAGG
AAGTAAAAGGCCTACAAGCCCAGATTGCCAGCTCTGGGTTGACCGTGGAGGTAGATGCCC
CCAAATCTCAGGACCTCGCCAAGATCATGGCAGACATCCGGGCCCAATATGACGAGCTGG
CTCGGAAGAACCGAGAGGAGCTAGACAAGTACTGGTCTCAGCAGATTGAGGAGAGCACCA
CAGTGGTCACCACACAGTCTGCTGAGGTTGGAGCTGCTGAGACGACGCTCACAGAGCTGA
GACGTACAGTCCAGTCCTTGGAGATCGACCTGGACTCCATGAGAAATCTGAAGGCCAGCT
TGGAGAACAGCCTGAGGGAGGTGGAGGCCCGCTACGCCCTACAGATGGAGCAGCTCAACG
GGATCCTGCTGCACCTTGAGTCAGAGCTGGCACAGACCCGGGCAGAGGGACAGCGCCAGG
CCCAGGAGTATGAGGCCCTGCTGAACATCAAGGTCAAGCTGGAGGCTGAGATCGCCACCT
ACCGCCGCCTGCTGGAAGATGGCGAGGACTTTAATCTTGGTGATGCCTTGGACAGCAGCA
ACTCCATGCAAACCATCCAAAAGACCACCACCCGCCGGATAGTGGATGGCAAAGTGGTGT
CTGAGACCAATGACACCAAAGTTCTGAGGCATTAAGCCAGCAGAAGCAGGGTACCCTTTG
GGGAGCAGGAGGCCAATAAAAAGTTCAGAGTTCAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_000224 unedited
ATTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGTCCGCAAAT
CCTGAGTCCTGTCCTTTCTCTCTCCCCGGACAGCATGAGCTTCACCACTCGCTCCACCTT
CTCCACCAACTACCGGTCCCTGGGCTCTGTCCAGGCGCCCAGCTACGGCGCCCGGCCGGT
CAGCAGCGCGGCCAGCGTCTATGCAGGCGCTGGGGGCTCTGGTTCCCGGATCTCCGTGTC
CCGCTCCACCAGCTTCAGGGGCGGCATGGGGTCCGGGGGCCTGGCCACCGGGATAGCCGG
GGGTCTGGCAGGAATGGGAGGCATCCAGAACGAGAAGGAGACCATGCAAAGCCTGAACGA
CCGCCTGGCCTCTTACCTGGACAGAGTGAGGAGCCTGGAGACCGAGAACCGGAGGCTGGA
GAGCAAAATCCGGGAGCACTTGGAGAAGAAGGGACCCCAGGTCAGAGACTGGAGCCATTA
CTTCAAGATCATCGAGGACCTGAGGGCTCAGATCTTCGCAAATACTGTGGACAATGCCCG
CATCGTTCTGCAGATTGACAATGCCCGTCTTGCTGCTGATGACTTTAGAGTCAAGTATGA
GACAGAGCTGGCCATGCGCCAGTCTGTGGAGAACGACATCCATGGGCTCCGCAAGGTCAT
TGATGACACCAATATCACACGACTGCAGCTGGAGACAGAGATCGAGGCTCTCAAGGAGGA
GCTGCTCTTCATGAAGAAGACCACGAAGAGGAAGTAAAAGGCCCTACAGCCCAGNATGCC
AGCTCTGGGGGTGACCGTGGAGGTANATGCCCCCAATCTCAGGACCTCGCCAAGATCATG
CAGACATCCGGCCCTATATGACGAGCTGGCTCGNAAGACCGAGAGGAGCTAGACAGTACT
GGTCTCC
>OriGene 3' read for NM_000224 unedited
GGCCGCATCTATATCGAGTTTTTTTTTTTTTTTTTTGAACTCTGAACTTTTTATTGGCCT
CCTGCTCCCCAAAGGTACCCTGCTTCTGCTGGCTTAATGCCTCAAAACTTTGGTGTCATT
GGTCTCAAACACCACTTTGCCATCCACTATCCGGCGGGTGGTGGTCTTTTGGATGGTTTG
CATGGAGTTGCTGCTGTCCAAGGCATCACCAAGATTAAAGTCCTCGCCATCTTCCAGCAG
GCGGTGGTAGGTGGCGAACTCAGCCTCCAACTTGACCTTGATGTTCAGCAGGGTCTAATA
CTACTGGGCCTGCCGCTGTTCATCTGCCCGGTTCTGTATCATCTCCGACCTCTGGGGCAA
CACGATTCTCCATGTCTCGCTCCCTCGTTAGGACTAGCGGACATCATCTCTCTTCTTCGC
TGCATCTTCCTCCCTACTTCAACTTCAATCACCTCAATATCAATTTTTTTCCTTACTACC
ACTTCATTTACTTTACCTTCATAATTTTCCCTCTTCTTTCCCCTCATCATCCCCTCTTCT
CCCTCTCGCTACTCCCTTAGTCTCTCTCTTCATTCTCACTATTACTTTTCTACCATCTCT
ACCTCCCATCACCATTTTCTCCTTGCATCCTTTCACTTCTTTCCCCCACCCACTTACTCC
TTCTAATCGCACACTTCAACAACCACCCTCCCCCTCCTCCCTCCTCATCTTCCCCTTCCT
CCTACCCTCCCCGTCCTCCTCCCGTCCTCTTTTTGCATATCTTTCTTTCATAACTTTGTC
CCACTACCCCCCCTTCTCTACCCCACTCTTCACCCTCACTTCCACCCTCTCTCAACCCCC
CATCACCCCTGTACTTTCGTCCTAACTCTTGTCGTCAAACTGTTCCTCTCACCTTCGCTC
CTTTATCGCCTCACTTTATT
Restriction Sites NotI-NotI     
ACCN NM_000224
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000224.2, NP_000215.1
RefSeq Size 1485 bp
RefSeq ORF 1293 bp
Locus ID 3875
Cytogenetics 12q13.13
Domains filament
Protein Pathways Pathogenic Escherichia coli infection
Gene Summary 'KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

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