Frataxin (FXN) (NM_000144) Human Untagged Clone

CAT#: SC120073

FXN (untagged)-Human frataxin (FXN), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FXN
• Synonyms: CyaY; FA; FARR; FRDA; X25
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120073 sequence for NM_000144 edited (data generated by NextGen Sequencing)
  ATGTGGACTCTCGGGCGCCGCGCAGTAGCCGGCCTCCTGGCGTCACCCAGCCCGGCCCAG
  GCCCAGACCCTCACCCGGGTCCCGCGGCCGGCAGAGTTGGCCCCACTCTGCGGCCGCCGT
  GGCCTGCGCACCGACATCGATGCGACCTGCACGCCCCGCCGCGCAAGTTCGAACCAACGT
  GGCCTCAACCAGATTTGGAATGTCAAAAAGCAGAGTGTCTATTTGATGAATTTGAGGAAA
  TCTGGAACTTTGGGCCACCCAGGCTCTCTAGATGAGACCACCTATGAAAGACTAGCAGAG
  GAAACGCTGGACTCTTTAGCAGAGTTTTTTGAAGACCTTGCAGACAAGCCATACACGTTT
  GAGGACTATGATGTCTCCTTTGGGAGTGGTGTCTTAACTGTCAAACTGGGTGGAGATCTA
  GGAACCTATGTGATCAACAAGCAGACGCCAAACAAGCAAATCTGGCTATCTTCTCCATCC
  AGTGGACCTAAGCGTTATGACTGGACTGGGAAAAACTGGGTGTACTCCCACGACGGCGTG
  TCCCTCCATGAGCTGCTGGCCGCAGAGCTCACTAAAGCCTTAAAAACCAAACTGGACTTG
  TCTTCCTTGGCCTATTCCGGAAAAGATGCTTGA
  
  Clone variation with respect to NM_000144.4
  54 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000144 unedited
  GTCAGCATTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGCCGGACAGCAT
  GTGGACTCTCGGGCGCCGCGCAGTAGCCGGCCTCCTGGCGTCACCCAGCCCGGCCCAGGC
  CCAGACCCTCACCCGGGTCCCGCGGCCGGCAGAGTTGGCCCCACTCTGCGGCCGCCGTGG
  CCTGCGCACCGACATCGATGCGACCTGCACGCCCCGCCGCGCAAGTTCGAACCAACGTGG
  CCTCAACCAGATTTGGAATGTCAAAAAGCAGAGTGTCTATTTGATGAATTTGAGGAAATC
  TGGAACTTTGGGCCACCCAGGCTCTCTAGATGAGACCACCTATGAAAGACTAGCAGAGGA
  AACGCTGGACTCTTTAGCAGAGTTTTTTGAAGACCTTGCAGACAAGCCATACACGTTTGA
  GGACTATGATGTCTCCTTTGGGAGTGGTGTCTTAACTGTCAAACTGGGTGGAGATCTAGG
  AACCTATGTGATCAACAAGCAGACGCCAAACAAGCAAATCTGGCTATCTTCTCCATCCAG
  TGGACCTAAGCGTTATGACTGGACTGGGAAAAACTGGGTGTACTCCCACGACGGCGTGTC
  CCTCCATGAGCTGCTGGCCGCAGAGCTCACTAAAGCCTTAAAAACCAAACTGGACTTGTC
  TTCCTTGGCCTATTCCGGAAAAGATGCTTGATGCCCAGCCCCGTTTTAAGGACATTANAA
  GCTATCAGGCCAAGACCCCAGCTTCATTATGCAGCTNGAGTCTGGTTTTTGGTGGTGGTG
  TTTATTTTTTTTATTCCTGCTNTTGAGGGACAGTGGGCTATGTGTCACAGCTCTGTAGAA
  GAATGTGTNGCCTNCTACCTGCCCCCAGTTCTGATTTTAAATTCTATGGAGATTTTTGGG
  ATGTCGATTTCTCCTNACATGAACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000144 unedited
  CGTACACTTGGACGCGGCCCATCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTATTATTATTATTATTTTATTGGTATTATTATTTTGAGACAG
  AGATTTTGCTCAGTCACCCAGGTTACAGGGCAATGGCACGATCTCGGCCCACTGGAACCT
  CCACCTCTGGGGTCAAGCGATTCTTCCGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGC
  CCCTGCCCTCATACCCGGGTAATTTTTTGGATTTTTAGTAAAGACGGGGTTTCATCATGT
  TGGCCAGGCTGGTCTTGAACTCCTGACCTCAGGGGATCCACCCACCTTGGCCTCCAAAAG
  TGCTGGGATTACCGGGGTGAGCCACCGCGCCAGGCCAAGGATGGCTATTATTTTCACCTG
  AATTCCACTGAAATCACGATTGGGCAACACCCATAACAATGTTTCCTATATAGCTTAAAA
  ACTGGTCTTTGTCATCCCCTCATCTACCCCATAGGGAATTAGAACTAGCCTGGTAGGTTG
  CTTGACCGGACCACTGAATGCTGGGAATCAACCATACCCTATTCTCTCATTTAACAGATG
  GGGAAACCGAGGTCCAGGGAATTTGGGAAACTTGTCCACCAAGGGACAACTACTTTGGGG
  CTGAACTATGTCTAGGACCAGGGGTCTCTTTATCAACATGAGTATCTCCCTCCTTTATCG
  GCCCCTTTCTGTTTACAAGGCACCTCCCCACTCCCTGGATTTTGTTTGTGCCCCTCCCTC
  CCTCCCCTCATGAGAACGGCCTTTTTTCCCCCTTTCCTTGGCCTGGGGAGAATTTTTGGC
  CTCCTGGGGGCCACCGGTTCCCAACCAACCCTTTAAAATGGGAAAAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000144
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000144.3, NP_000135.2
• RefSeq Size: 2262 bp
• RefSeq ORF: 633 bp
• Locus ID: 2395
• Cytogenetics: 9q21.11
• Domains: Frataxin_Cyay
• Protein Families: Druggable Genome
• Gene Summary: 'This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.