glucose 6 phosphatase, catalytic subunit (G6PC) (NM_000151) Human Untagged Clone

CAT#: SC120076

G6PC (untagged)-Human glucose-6-phosphatase, catalytic subunit (G6PC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: G6PC
• Synonyms: G6Pase; G6PC1; G6PT; GSD1; GSD1a
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120076 sequence for NM_000151 edited (data generated by NextGen Sequencing)
  ATGGAGGAAGGAATGAATGTTCTCCATGACTTTGGGATCCAGTCAACACATTACCTCCAG
  GTGAATTACCAAGACTCCCAGGACTGGTTCATCTTGGTGTCCGTGATCGCAGACCTCAGG
  AATGCCTTCTACGTCCTCTTCCCCATCTGGTTCCATCTTCAGGAAGCTGTGGGCATTAAA
  CTCCTTTGGGTAGCTGTGATTGGAGACTGGCTCAACCTCGTCTTTAAGTGGATTCTCTTT
  GGACAGCGTCCATACTGGTGGGTTTTGGATACTGACTACTACAGCAACACTTCCGTGCCC
  CTGATAAAGCAGTTCCCTGTAACCTGTGAGACTGGACCAGGGAGCCCCTCTGGCCATGCC
  ATGGGCACAGCAGGTGTATACTACGTGATGGTCACATCTACTCTTTCCATCTTTCAGGGA
  AAGATAAAGCCGACCTACAGATTTTGGTGCTTGAATGTCATTTTGTGGTTGGGATTCTGG
  GCTGTGCAGCTGAATGTCTGTCTGTCACGAATCTACCTTGCTGCTCATTTTCCTCATCAA
  GTTGTTGCTGGAGTCCTGTCAGGCATTGCTGTTGCAGAAACTTTCAGCCACATCCACAGC
  ATCTATAATGCCAGCCTCAAGAAATATTTTCTCATTACCTTCTTCCTGTTCAGCTTCGCC
  ATCGGATTTTATCTGCTGCTCAAGGGACTGGGTGTAGACCTCCTGTGGACTCTGGAGAAA
  GCCCAGAGGTGGTGCGAGCAGCCAGAATGGGTCCACATTGACACCACACCCTTTGCCAGC
  CTCCTCAAGAACCTGGGCACGCTCTTTGGCCTGGGGCTGGCTCTCAACTCCAGCATGTAC
  AGGGAGAGCTGCAAGGGGAAACTCAGCAAGTGGCTCCCATTCCGCCTCAGCTCTATTGTA
  GCCTCCCTCGTCCTCCTGCACGTCTTTGACTCCTTGAAACCCCCATCCCAAGTCGAGCTG
  GTCTTCTACGTCTTGTCCTTCTGCAAGAGTGCGGTAGTGCCCCTGGCATCCGTCAGTGTC
  ATCCCCTACTGCCTCGCCCAGGTCCTGGGCCAGCCGCACAAGAAGTCGTTGTAA
  
  Clone variation with respect to NM_000151.2
  445 c=>t;574 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000151 unedited
  GCATATGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGACCACCAAGCCTG
  GATAACTGCAGGGCTCTGCTGACATCTTCCTGAGGTGCCAAGGAAATGAGGATGGAGGAA
  GGAATGAATGTTCTCCATGACTTTGGGATCCAGTCAACACATTACCTCCAGGTGAATTAC
  CAAGACTCCCAGGACTGGTTCATCTTGGTGTCCGTGATCGCAGACCTCAGGAATGCCTTC
  TACGTCCTCTTCCCCATCTGGTTCCATCTTCAGGAAGCTGTGGGCATTAAACTCCTTTGG
  GTAGCTGTGATTGGAGACTGGCTCAACCTCGTCTTTAAGTGGATTCTCTTTGGACAGCGT
  CCATACTGGTGGGTTTTGGATACTGACTACTACAGCAACACTTCCGTGCCCCTGATAAAG
  CAGTTCCCTGTAACCTGTGAGACTGGACCAGGGAGCCCCTCTGGCCATGCCATGGGCACA
  GCAGGTGTATACTACGTGATGGTCACATCTACTCTTTCCATCTTTCAGGGAAAGATAAAG
  CCGACCTACAGATTTTGGTGCTTGAATGTCATTTTGTGGTTGGGATTCTGGGCTGTGCAG
  CTGAATGTCTGTCTGTCACGAATCTACCTTGCTGCTCATTNTCCTCATCAAGTTGTTGCT
  GGAGTCCTGTCAGGCATTGCTGTTGCAGAAACTTTCAGCCACATCCACAGCATCTATAAT
  GCCAGCCTCAAGAAATATTTCTCATTACCTTNCTCCTGTTCAGCTTCGCCATCGGATTNT
  ATCTGCTGCTCAGGGACTGGGTGTAGACCTCCTGTGGACTCTGCAGAAAGCCCCCAGTGG
  TGCGAGCAGCCAGAATGGGTCCCATTGACACACACCTTTGCCAGCCCCTCAAGACCTGGC
  ACGCTCTTTGCCTGGGCCTGCTCTCACTCCCCTGTCAAGGAGAGCGCAGGNGAACTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000151 unedited
  AGTTTGGCCGCGGCCGCAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTATAGGAATTAACAAGACGTGATTCTTAAATAAAATGTTGCTTTAATAAAATACT
  GCACATATATATTTTTCTTTTCAAAGATGTTATGAGCAGGATGGTTGTTTTGAATGATAA
  TGTATGGTATACTATATAATTAGTAGCAAAAACAAGGACTTTCAAATAAATAGGCTTCAT
  CTTTTTTTGGGGGGTAGGTGGGTGGGGCAAAAATACTCTGCATATCAGATGAAATTGAAA
  AACAGGCAAGGAGGGCCCGGTGCGGGGGCTCATGCCTGTAATCCTAGCACCTTGGGAGGC
  CAAGGCAGGTGGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGATGAAA
  TCCCATCTCTTCTAAAAATATAAAAATTAGCCTGGCATGGTGGTGCACGCCGGTAGTCCT
  AGCTACTGAGGAGGCTGAGACATGAGAATCGCTTGAACCAGGAGGAAGAGGTTGCAGTGA
  GCCAAGATCGTGCCACTCCACTCCAGCCTGGGTGATAGGTGCGCCGTCTCAAAAAAAGAA
  AAAAAAAGAAAAGCAGGCAAGGAGGAGTTGGCTAGCTGGACATAGTGGCTTGCACCTGTA
  GTCCCAGCTACGCGGGACGCTGTGGTATGGTGGGAGGACTGCTTGAGCCCANGAATTCAG
  AGCAGCCTGGGCAACATAGTGAGACCCTGTCTCAAAAAAAAAAAAAAGAGAGAGAATGTG
  ACTTTTCCTTTAAAATGTGCTCTTGGNTGGCCTTGGGATAAAGAAAGGATTCTATTTTAC
  CCTCTAGGAATAAAAGTTACACTGGAATGACCTGAAAAAGGATTCTGGNCCTCCCCTTGA
  ATTTTGCCAACAATTCAGACCCTATTGGAACTACAAAGCCCCACGGGTCGCCTCTAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000151
• Insert Size: 3110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000151.1, NP_000142.1
• RefSeq Size: 3095 bp
• RefSeq ORF: 1074 bp
• Locus ID: 2538
• Cytogenetics: 17q21.31
• Domains: acidPPc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism
• Gene Summary: 'Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.