GLDC (NM_000170) Human Untagged Clone

CAT#: SC120090

GLDC (untagged)-Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLDC
• Synonyms: GCE; GCSP; HYGN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000170 edited
  GGCACGAGGGCAGCGCCCATTCATTGCCCGCGAGCGTCCATCCATCTGTCCGGCCGACTG
  TCCAGCGAAAGGGGCTCCAGGCCGGGCGCAGCCGCCACCCGGGGGACCGAGGCCAGGAGA
  GGGGCCAAGAGCGCGGCTGACCCTTGCGGGCCGGGGCAGGGGACGGTGGCCGCGGCCATG
  CAGTCCTGTGCCAGGGCGTGGGGGCTGCGCCTGGGCCGCGGGGTCGGGGGCGGCCGCCGC
  CTGGCTGGGGGATCGGGGCCGTGCTGGGCGCCGCGGAGCCGGGACAGCAGCAGTGGCGGC
  GGGGACAGCGCCGCGGCTGGGGCCTCGCGCCTCCTGGAGCGCCTTCTGCCCAGACACGAC
  GACTTCGCTCGGAGGCACATCGGCCCTGGGGACAAAGACCAGAGAGAGATGCTGCAGACC
  TTGGGGCTGGCGAGCATTGATGAATTGATCGAGAAGACGGTCCCTGCCAACATCCGTTTG
  AAAAGACCCTTGAAAATGGAAGACCCTGTTTGTGAAAATGAAATCCTTGCAACTCTGCAT
  GCCATTTCAAGCAAAAACCAGATCTGGAGATCGTATATTGGCATGGGCTATTATAACTGC
  TCAGTGCCACAGACGATTTTGCGGAACTTACTGGAGAACTCAGGATGGATCACCCAGTAT
  ACTCCATACCAGCCTGAGGTGTCTCAGGGGAGGCTGGAGAGTTTACTCAACTACCAGACC
  ATGGTGTGTGACATCACAGGCCTGGACATGGCCAATGCATCCCTGCTGGATGAGGGGACT
  GCAGCCGCAGAGGCACTGCAGCTGTGCTACAGACACAACAAGAGGAGGAAATTTCTCGTT
  GATCCCCGTTGCCACCCACAGACAATAGCTGTTGTCCAGACTCGAGCCAAATATACTGGA
  GTCCTCACTGAGCTGAAGTTACCCTGTGAAATGGACTTCAGTGGAAAAGATGTCAGTGGA
  GTGTTGTTCCAGTACCCAGACACGGAGGGGAAGGTGGAAGACTTTACGGAACTCGTGGAG
  AGAGCTCATCAGAGTGGGAGCCTGGCCTGCTGTGCTACTGACCTTTTAGCTTTGTGCATC
  TTGAGGCCACCTGGAGAATTTGGGGTAGACATCGCCCTGGGCAGCTCCCAGAGATTTGGA
  GTGCCACTGGGCTATGGGGGACCCCATGCAGCATTTTTTGCTGTCCGAGAAAGCTTGGTG
  AGAATGATGCCTGGAAGAATGGTGGGGGTAACAAGAGATGCCACTGGGAAAGAAGTGTAT
  CGTCTTGCTCTTCAAACCAGGGAGCAACACATTCGGAGAGACAAGGCTACCAGCAACATC
  TGTACAGCTCAGGCCCTCTTGGCGAATATGGCTGCCATGTTTGCAATCTACCATGGTTCC
  CATGGGCTGGAGCATATTGCTAGGAGGGTACATAATGCCACTTTGATTTTGTCAGAAGGT
  CTCAAGCGAGCAGGGCATCAACTCCAGCATGACCTGTTCTTTGATACCTTGAAGATTCAG
  TGTGGCTGCTCAGTGAAGGAGGTCTTGGGCAGGGCCGCTCAGCGGCAGATCAATTTTCGG
  CTTTTTGAGGATGGCACACTTGGTATTTCTCTTGATGAAACAGTCAATGAAAAAGATCTG
  GACGATTTGTTGTGGATCTTTGGTTGTGAGTCATCTGCAGAACTGGTTGCTGAAAGCATG
  GGAGAGGAGTGCAGAGGTATTCCAGGGTCTGTGTTCAAGAGGACCAGCCCGTTCCTCACC
  CATCAAGTGTTCAACAGCTACCACTCTGAAACAAACATTGTCCGGTACATGAAGAAACTG
  GAAAATAAAGACATTTCCCTTGTTCACAGCATGATTCCACTGGGATCCTGCACCATGAAA
  CTGAACAGTTCGTCTGAACTCGCACCTATCACATGGAAAGAATTTGCAAACATCCACCCC
  TTTGTGCCTCTGGATCAAGCTCAAGGATATCAGCAGCTTTTCCGAGAGCTTGAGAAGGAT
  TTGTGTGAACTCACAGGTTATGACCAGGTCTGTTTCCAGCCAAACAGCGGAGCCCAGGGA
  GAATATGCTGGACTGGCCACTATCCGAGCCTACTTAAACCAGAAAGGAGAGGGGCACAGA
  ACGGTTTGCCTCATTCCGAAATCAGCACATGGGACCAACCCAGCAAGTGCCCACATGGCA
  GGCATGAAGATTCAGCCTGTGGAGGTGGATAAATATGGGAATATCGATGCAGTTCACCTC
  AAGGCCATGGTGGATAAGCACAAGGAGAACCTAGCAGCTATCATGATTACATACCCATCC
  ACCAATGGGGTGTTTGAAGAGAACATCAGTGACGTGTGTGACCTCATCCATCAACATGGA
  GGACAGGTCTACCTAGACGGGGCAAATATGAATGCTCAGGTGGGAATCTGTCGCCCTGGA
  GACTTCGGGTCTGATGTCTCGCACCTAAATCTTCACAAGACCTTCTGCATTCCCCACGGA
  GGAGGTGGTCCTGGCATGGGGCCCATCGGAGTGAAGAAACATCTCGCCCCGTTTTTGCCC
  AATCATCCCGTCATTTCACTAAAGCGGAATGAGGATGCCTGTCCTGTGGGAACCGTCAGT
  GCGGCCCCATGGGGCTCCAGTTCCATCTTGCCCATTTCCTGGGCTTATATCAAGATGATG
  GGAGGCAAGGGTCTTAAACAAGCCACGGAAACTGCGATATTAAATGCCAACTACATGGCC
  AAGCGATTAGAAACACACTACAGAATTCTTTTCAGGGGTGCAAGAGGTTATGTGGGTCAT
  GAATTTATTTTGGACACGAGACCCTTCAAAAAGTCTGCAAATATTGAGGCTGTGGATGTG
  GCCAAGAGACTCCAGGATTATGGATTTCACGCCCCTACCATGTCCTGGCCTGTGGCAGGG
  ACCCTCATGGTGGAGCCCACTGAGTCGGAGGACAAGGCAGAGCTGGACAGATTCTGTGAT
  GCCATGATCAGCATTCGGCAGGAAATTGCTGACATTGAGGAGGGCCGCATCGACCCCAGG
  GTCAATCCGCTGAAGATGTCTCCACACTCCCTGACCTGCGTTACATCTTCCCACTGGGAC
  CGGCCTTATTCCAGAGAGGTGGCAGCATTCCCACTCCCCTTCGTGAAACCAGAGAACAAA
  TTCTGGCCAACGATTGCCCGGATTGATGACATATATGGAGATCAGCACCTGGTTTGTACC
  TGCCCACCCATGGAAGTTTATGAGTCTCCATTTTCTGAACAAAAGAGGGCGTCTTCTTAG
  TCCTCTCTCCCTAAGTTTAAAGGACTGATTTGATGCCTCTCCCCAGAGCATTTGATAAGC
  AAGAAAGATTTCATCTCCCACCCCAGCCTCAAGTAGGAGTTTTATATACTGTGTATATCT
  CTGTAATCTCTGTCAAGGTAAATGTAAATACAGTAGCTGGAGGGAGTCGAAGCTGATGGT
  TGGAAGACGGATTTGCTTTGGTATTCTGCTTCCACATGTGCCAGTTGCCTGGATTGGGAG
  CCATTTTGTGTTTTGCGTAGAAAGTTTTAGGAACTTTAACTTTTAATGTGGCAAGTTTGC
  AGATGTCATAGAGGCTATCCTGGAGACTTAATAGACATTTTTTTGTTCCAAAAGAGTCCA
  TGTGGACTGTGCCATCTGTGGGAAATCCCAGGGCAAATGTTTACATTTTGTATACCCTGA
  AGAACTCTTTTTCCTCTAATATGCCTAATCTGTAATCACATTTCTGAGTGTTTTCCTCTT
  TTTCTGTGTGAGGTTTTTTTTTTTTAATCTGCATTTATTAGTATTCTAATAAAAGCTTTT
  GATCGGAAAAAAAAAAAAAAAAAAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000170 unedited
  GGTCACAATTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCAGCGCCCA
  TTCATTGCCCGCGAGCGTCCATCCATCTGTCCGGCCGACTGTCCAGCGAAAGGGGCTCCA
  GGCCGGGCGCAGCCGCCACCCGGGGGACCGAGGCCAGGAGAGGGGCCAAGAGCGCGGCTG
  ACCCTTGCGGGCCGGGGCAGGGGACGGTGGCCGCGGCCATGCAGTCCTGTGCCAGGGCGT
  GGGGGCTGCGCCTGGGCCGCGGGGTCGGGGGCGGCCGCCGCCTGGCTGGGGGATCGGGGC
  CGTGCTGGGCGCCGCGGAGCCGGGACAGCAGCAGTGGCGGCGGGGACAGCGCCGCGGCCT
  GGGGGCCTCGCGCCTCCTGGAGCGCCTTCTGCCCAGACACGACGACTTCGCTCGGAGGCA
  CATCGGCCCTGGGGACAAAGACCAGAGAGAGATGCTGCAGACCTTGGGGCTGGCGAGCAT
  TGATGAATTGATCGAGAAGACGGTCCCTGCCAACATCCGTTTGAAAAGACCCTTGAAAAT
  GGAAGACCCTGTTTGTGAAAATGAAATCCTTGCAACTCTGCATGCCATTTCAAGCAAAAA
  CCAGATCTGGAGATCGTATATTGGCATGGGCTATTAATCGGGGGTTCAATGCCACAGACG
  ATTTTGCGGAACTTACTGGAGAACTCANGATGGATCACCCAGTATACTCCATTACCAGCC
  TGAGTGTCTCAGGNGAGGCTGNGAGAGTTACTCAACTACCAGACCATGGGTGTGTGACAT
  CACAGGCCTGGACATGGCCAATGCATCCCTGCTGGATGAAGGGGACTGCAGCCGCANAAG
  CACTGCAGCTGTGCTACAGACACAACAAGAGGGAGGAAATTTCTCGTTGATCCCCGTTGC
  CACCACAGACAATACCTGTTGTTCAGACTCGAGCCAAATTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000170 unedited
  TATGGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTCCGATCAAAAG
  CTTTTATTAGAATACTAATAAATGCAGATTAAAAAAAAAAAACCTCACACAGAAAAAGAG
  GAAAACACTCAGAAATGTGATTACAGATTAGGCATATTAGAGGAAAAAGAGTTCTTCAGG
  GTATACAAAATGTAAACATTTGCCCTGGGATTTCCCACAGATGGCACAGTCCACATGGAC
  TCTTTTGGAACAAAAAAATGTCTATTAAGTCTCCAGGATAGCCTCTATGACATCTGCAAA
  CTTGCCACATTAAAAGTTAAAGTTCCTAAAACTTTCTACGCAAAACACAAAATGGCTCCC
  AATCCAGGCAACTGGCACATGTGGAAGCAGAATACCAAAGCAAATCCGTCTTCCAACCAT
  CNAGCTTCGACTTCCTTCCCACCTACTGGAATTTAACCTTTTAACCCTTGGGGCGGGGTT
  TTTCCCCGGGTATTTTCCCCCGGGTTTTTTAAAACCCCCCTTTTGGGGGGGGGGGGGGGG
  AAAAAAAATTTTTTTGGTCTTAAAAGCGCCCGGGGGGGAGGGGGCAAAAAAACCCCCTCT
  TTTAACCTGTTTGGGGGGGAGGGAGGGCCCTTTAAAAAACCCCCCCCCTTTTTTTTTTTT
  TAAAAAAAAGGGGGGGGGCTCTCTTCCTTCCCTCCCCCCCGGGGGGGGGGGGGGGGGGGA
  AAAAAAAAAAGGGGGGGGGGGCCCTCTTTTATTTTTTAAAACTTCCGGCGGGCCTGTGGG
  GGGCCAAAAAAATATTTTTTTTTTTTTTTTTCTTGGGGGGGGGGGGGGGGGGGCCCCCCC
  CCCCTCTTTATAAATAAACCCCCCCCCCCCCAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000170
• Insert Size: 3700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000170.1, NP_000161.1
• RefSeq Size: 3783 bp
• RefSeq ORF: 3063 bp
• Locus ID: 2731
• Cytogenetics: 9p24.1
• Domains: GDC-P
• Protein Families: Druggable Genome
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways
• Gene Summary: 'Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq, Jan 2010]'