RFT1 (NM_052859) Human Untagged Clone

CAT#: SC120145

RFT1 (untagged)-Human RFT1 homolog (S. cerevisiae) (RFT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFT1
• Synonyms: CDG1N
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_052859, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAGCCAGGAGGTGCTGGGCCACGCGGCCCGGCTGGCCTCCTCCGGTCTCCTCCTGCAGGTGTTGT
  TTCGGTTGATCACCTTTGTCTTGAATGCATTTATTCTTCGCTTCCTGTCAAAGGAAATCGTTGGCGTAGT
  AAATGTAAGACTAACGCTGCTTTACTCAACCACCCTCTTCCTGGCCAGAGAGGCCTTCCGCAGAGCATGT
  CTCAGTGGGGGCACCCAGCGAGACTGGAGCCAGACCCTCAACCTGCTGTGGCTAACAGTCCCCCTGGGTG
  TGTTTTGGTCCTTATTCCTGGGCTGGATCTGGTTGCAGCTGCTTGAAGTGCCTGATCCTAATGTTGTCCC
  TCACTATGCAACTGGAGTGGTGCTGTTTGGTCTCTCGGCAGTGGTGGAGCTTCTAGGAGAGCCCTTTTGG
  GTCTTGGCACAAGCACATATGTTTGTGAAGCTCAAGGTGATTGCAGAGAGCCTGTCGGTAATTCTTAAGA
  GCGTTCTGACAGCTTTTCTCGTGCTGTGGTTGCCTCACTGGGGATTGTACATTTTCTCTTTGGCCCAGCT
  TTTCTATACCACAGTTCTGGTGCTCTGCTATGTTATTTATTTCACAAAGTTACTGGGTTCCCCAGAATCA
  ACCAAGCTTCAAACTCTTCCTGTCTCCAGAATAACAGATCTGTTACCCAATATTACAAGAAATGGAGCGT
  TTATAAACTGGAAAGAGGCTAAACTGACTTGGAGTTTTTTCAAACAGTCTTTCTTGAAACAGATTTTGAC
  AGAAGGCGAGCGATATGTGATGACATTTTTGAATGTATTGAACTTTGGTGATCAGGGTGTGTATGATATA
  GTGAATAATCTTGGCTCCCTTGTGGCCAGATTAATTTTCCAGCCAATAGAGGAAAGTTTTTATATATTTT
  TTGCTAAGGTGCTGGAGAGGGGAAAGGATGCCACACTTCAGAAGCAGGAGGACGTTGCTGTGGCTGCTGC
  AGTCTTGGAGTCCCTGCTCAAGCTGGCCCTGCTGGCCGGCCTGACCATCACTGTTTTTGGCTTTGCCTAT
  TCTCAGCTGGCTCTGGATATCTACGGAGGGACCATGCTTAGCTCAGGATCCGGTCCTGTTTTGCTGCGTT
  CCTACTGTCTCTATGTTCTCCTGCTTGCCATCAATGGAGTGACAGAGTGTTTCACATTTGCTGCCATGAG
  CAAAGAGGAGGTCGACAGGTACAATTTTGTGATGCTGGCCCTGTCCTCCTCATTCCTGGTGTTATCCTAT
  CTCTTGACCCGTTGGTGTGGCAGCGTGGGCTTCATCTTGGCCAACTGCTTTAACATGGGCATTCGGATCA
  CGCAGAGCCTTTGCTTCATCCACCGCTACTACCGAAGGAGCCCCCACAGGCCCCTGGCTGGCCTGCACCT
  ATCGCCAGTCCTGCTCGGGACATTTGCCCTCAGTGGTGGGGTTACTGCTGTTTCGGAGGTATTCCTCTGC
  TGTGAGCAGGGCTGGCCAGCCAGACTGGCACACATTGCTGTGGGGGCCTTCTGTCTGGGAGCAACTCTCG
  GGACAGCATTCCTCACAGAGACCAAGCTGATCCATTTCCTCAGGACTCAGTTAGGTGTGCCCAGACGCAC
  TGACAAAATGACATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_052859 unedited
  TTTTGGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGCGCGGAGGC
  TATGGGCAACCAGGAGGTGCTGGGCCACGCGGCCCGGCTGGCCTCCTCCGGTCTCCTCCT
  GCAGGTGTTGTTTCGGCTGATCACCTTTGTCTTGAATGCATTTATTCTTCGCTTCCTGTC
  AAAGGAAATCGTTGGCGTAATAAATGTAAGACTAACGCTGCTTTACTCAACCACCCTCTT
  CCTGGCCAGAGAGGCCTTCCGCATAGCATGTCTCAGCGGGGGCACCCAACGAGACTGGAG
  CCAGACCCTCAACCTGCTGTGGCTAACAGTCCCCCTGGGTGTGTTTTGGTCCTTATTCCT
  GGGCTGGATCTGGTTGCAGCTGCTTGAAGTGCCTGATCCTAATGTTGACCCTCACTATGC
  AACTGGAGTGGTGCTGATAGGTCTCTCGGCAGAGGTGGAGCTTCTAGGAGAGCCCTTTTG
  GGTCTTGGCACAAGCACATATGTCCGCGAAGCTCAAGGTGATTGCAGAGAGCCTGTCGGT
  AATTCTTAAGAGCGGTCTGACAGCTTTTCTCGCGCTGCGGTTGCCTCACTGAGGATTGCA
  CATTATCTCTTTGGCCCAGCTTTTCTATACCACACTTCTGGCGCTCTGCTATGTTATTTA
  TTTCACATAGTTACTGGGATACCCACAATCAACCAAGCTTGAAACTCTTCCTGTCTACAC
  AATAACAGATCTGCTACCCAATATGTACAGACTATGGAGCGTTATAAACTGGCAAGACGC
  TAAACTGACTGGNAGTATATTCAAACAGTCTCTCTTTGAACAGACTNTGTCAGCATGCGA
  GCGATATGTGATGACATTTTTGTATGCATTGAACGTTCGTGACCAGGGTGCTGTATGATA
  TAGGAAATATCTCGACTTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_052859 unedited
  CATGGNGATGGCACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATGCCACC
  CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTT
  TTTTTTTTTCCAGGGCCACACCCTGTTTCCATGAGCTGCATGAAAGAGAGAGGTTTCTCT
  CTCTCTTTCACCGAAAAACTTCATTGTTCCTGTGAAGGGTAAAATCACTGCTTCTCTTTT
  TCTGGGCCAGATGCTTATTCCCAGTTAACATATAGGATACAGCTTGTTGGAGCATGTGAC
  TCCAAAGGCCAATCTTCCGCTGAAGGACTGATTCTCTTACTCAAAGAGAAGATGCTGGCT
  ACTATCAACTGATGCACAACTATTATTTCTAAAGGGCTTTCGGTCTTCACTTAAAAATGA
  CACTCCCCCGCCGCATTACACACTTCTAATGGGCACAGGTGTCTCATGCACTGACACTCT
  CTGTGGCCTCATCTCTGGGGTTGCTGTCTCTCCGCTGCAGAGCCCTCAGTGAGGCTCTTA
  CACAGAATGTGTGCTACCCACAGACTACCCATAGCTGGTCCAGGTGCCTCAGATGTACAG
  GCTTCCCTGAGTCAGGTCATTTTGTCAGTGCCGTTAGGCACACCTAACTGTGTCCTGGAG
  AACGTATCCAGCTTGACTCTGTGAGGAATGCGGTTCAAGTGTTGCTACCATACACAGGCC
  CGCACAATCATGTGTGCCATTCAGCATGGCCTTCCTTGCTTAGACTTGATGAGTCCCTGA
  AACCAGGGCTAACCCTCCTCTTGAGGCAAAGGTCCCAAATAGGACTGAGGATTGTGCCAG
  CACAACCCGGTCCTTGGGGGAGCTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_052859
• ORF Size: 1626 bp
• Insert Size: 1700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_052859.2, NP_443091.1
• RefSeq Size: 2810
• RefSeq ORF: 1626
• Locus ID: 91869
• Domains: Rft-1
• Protein Families: Transmembrane
• Protein Pathways: N-Glycan biosynthesis
• Gene Summary: This gene encodes an enzyme which catalyzes the translocation of the Man(5)GlcNAc (2)-PP-Dol intermediate from the cytoplasmic to the luminal side of the endoplasmic reticulum membrane in the pathway for the N-glycosylation of proteins. Mutations in this gene are associated with congenital disorder of glycosylation type In. [provided by RefSeq, Dec 2008]