RFT1 (NM_052859) Human Untagged Clone

CAT#: SC324324

RFT1 (untagged)-Human RFT1 homolog (S. cerevisiae) (RFT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFT1
• Synonyms: CDG1N
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_052859.2 CGGAGTGGCGGCGGCATTTCCTGGTGTCTGAGCCTGGCGCGGAGGCTATGGGCAGCCAGG
  AGGTGCTGGGCCACGCGGCCCGGCTGGCCTCCTCCGGTCTCCTCCTGCAGGTGTTGTTTC
  GGTTGATCACCTTTGTCTTGAATGCATTTATTCTTCGCTTCCTGTCAAAGGAAATCGTTG
  GCGTAGTAAATGTAAGACTAACGCTGCTTTACTCAACCACCCTCTTCCTGGCCAGAGAGG
  CCTTCCGCAGAGCATGTCTCAGTGGGGGCACCCAGCGAGACTGGAGCCAGACCCTCAACC
  TGCTGTGGCTAACAGTCCCCCTGGGTGTGTTTTGGTCCTTATTCCTGGGCTGGATCTGGT
  TGCAGCTGCTTGAAGTGCCTGATCCTAATGTTGTCCCTCACTATGCAACTGGAGTGGTGC
  TGTTTGGTCTCTCGGCAGTGGTGGAGCTTCTAGGAGAGCCCTTTTGGGTCTTGGCACAAG
  CACATATGTTTGTGAAGCTCAAGGTGATTGCAGAGAGCCTGTCGGTAATTCTTAAGAGCG
  TTCTGACAGCTTTTCTCGTGCTGTGGTTGCCTCACTGGGGATTGTACATTTTCTCTTTGG
  CCCAGCTTTTCTATACCACAGTTCTGGTGCTCTGCTATGTTATTTATTTCACAAAGTTAC
  TGGGTTCCCCAGAATCAACCAAGCTTCAAACTCTTCCTGTCTCCAGAATAACAGATCTGT
  TACCCAATATTACAAGAAATGGAGCGTTTATAAACTGGAAAGAGGCTAAACTGACTTGGA
  GTTTTTTCAAACAGTCTTTCTTGAAACAGATTTTGACAGAAGGCGAGCGATATGTGATGA
  CATTTTTGAATGTATTGAACTTTGGTGATCAGGGTGTGTATGATATAGTGAATAATCTTG
  GCTCCCTTGTGGCCAGATTAATTTTCCAGCCAATAGAGGAAAGTTTTTATATATTTTTTG
  CTAAGGTGCTGGAGAGGGGAAAGGATGCCACACTTCAGAAGCAGGAGGACGTTGCTGTGG
  CTGCTGCAGTCTTGGAGTCCCTGCTCAAGCTGGCCCTGCTGGCCGGCCTGACCATCACTG
  TTTTTGGCTTTGCCTATTCTCAGCTGGCTCTGGATATCTACGGAGGGACCATGCTTAGCT
  CAGGATCCGGTCCTGTTTTGCTGCGTTCCTACTGTCTCTATGTTCTCCTGCTTGCCATCA
  ATGGAGTGACAGAGTGTTTCACATTTGCTGCCATGAGCAAAGAGGAGGTCGACAGGTACA
  ATTTTGTGATGCTGGCCCTGTCCTCCTCATTCCTGGTGTTATCCTATCTCTTGACCCGTT
  GGTGTGGCAGCGTGGGCTTCATCTTGGCCAACTGCTTTAACATGGGCATTCGGATCACGC
  AGAGCCTTTGCTTCATCCACCGCTACTACCGAAGGAGCCCCCACAGGCCCCTGGCTGGCC
  TGCACCTATCGCCAGTCCTGCTCGGGACATTTGCCCTCAGTGGTGGGGTTACTGCTGTTT
  CGGAGGTATTCCTCTGCTGTGAGCAGGGCTGGCCAGCCAGACTGGCACACATTGCTGTGG
  GGGCCTTCTGTCTGGGAGCAACTCTCGGGACAGCATTCCTCACAGAGACCAAGCTGATCC
  ATTTCCTCAGGACTCAGTTAGGTGTGCCCAGACGCACTGACAAAATGACATGACTTCAGG
  GAAGCCTGGACACCCGAGGCACCTGGACCAGCTATGGGTAGTTCTGTGGGTGGAACACAT
  TCTGTGTAAGAGCCCCACTGAGGGCTCTGCAGCGGAGTGACAGCAACCCCAGAGATGAGG
  CACCAGAGAGTGCCACTGCATGAGACACCTGTGACCATTCGAAGTCTGAAATGCGGGGGG
  GGAGTTTCATTTTTAAGTGAAGACCAAAAGCCCTTTAAAAATAATAGTTTTTTATCATTT
  TATAGTAATCAGCATTTTCTCTTTTACTAATATACTCATTCCTTTTGCGTATGATTGGCC
  TTTGGAGCTACATGCTCCAACAAACTATATCTTAATTGTTAACTGTTAATAATTATCTGG
  CCCAGAAAAAGAGATGCAGTGATTTTACCCTTCACAGGAATATTGAACTTCTTCTGTAAA
  AGAGAGAGAGAAACCTCTCTCTTTCATGCACCACATGGAAACAGAGTGTGGCCCTGAAGT
  TGTGGCTTTCTTTGTGTCAAAACTGTGGTCTGAGTTTTTAGAGAAGGAAAAATGCTACTC
  AGTGAGAAATCCAAGAAATGAAACCAGGAAGGTTTTCAGATCACATTTCCCAGAGTGAGT
  TCGGGAGAATCTTGGCTGTCGTGATCACACTGTTCCCCGCCGCCCCAGGTCAGCACATAC
  TTGACCCAGAGTGGGCTCTCAGGATTTGTTGATTGAAGGGTGGGTGAAAGCTGGCTGGAG
  ACTCCTGGCCTGGCCACCTGCAAGAGGCGGACTTTGAAGAAAACTGTGATCCAGTTGAGC
  CACTTTGTCCCTGAGGGGAATGCTGCTTTGCAGCTTTCTGTAACTTCTCACATGGGAATT
  GCACTGGTGACAGATAAGGTTTAACAGATGTACTTTTTTATCCTCAGAATAAGACAAGGC
  AAACTCTATATGGCTAGTATTACTGAGTCAGGTCCCTCCCGTTGACCCATTAATTCCAGC
  ATTGATCAATACAACCAAATTCCAGCCCCAGGACAAGAGTGGCAGGCATGGGGGCCTGGT
  ACTCCAGGCTACAGTGTGAGCCCAGGTGTTCTGCACCTTGCAGCCTGCACACTGCAGGTG
  AAATCATGAGCAGTGTTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_052859.2 unedited
  TGCTCAGCCTGTACGCCCTTCCCGCTTGTTTTGACCTCCATAGAAGACACCGGGACCGAT
  TCCAGCCTCCGGACTCTAGCCTAGGCCGCGGGACGGATAACAATTTCACACAGGAAACAG
  CTATGACCATTAGGCCTATTTAGGTGACACTATAGAACAAGTTTGTACAAAAAAGCAGGC
  TGGTACCGGTCCGGAATTCCCGGGATCGGAGTGGCGGCGGCATTTCCTGGTGTCTGAGCC
  TGGCGCGGAGGCTATGGGCAGCCAGGAGGTGCTGGGCCACGCGGCCCGGCTGGCCTCCTC
  CGGTCTCCTCCTGCAGGTGTTGTTTCGGTTGATCACCTTTGTCTTGAATGCATTTATTCT
  TCGCTTCCTGTCAAAGGAAATCGTTGGCGTAGTAAATGTAAGACTAACGCTGCTTTACTC
  AACCACCCTCTTCCTGGCCAGAGAGGCCTTCCGCAGAGCATGTCTCAGTGGGGGCACCCA
  GCGAGACTGGAGCCAGACCCTCAACCTGCTGTGGCTAACAGTCCCCCTGGGTGTGTTTTG
  GTCCTTATTCCTGGGCTGGATCTGGTTGCAGCTGCTTGAAGTGCCTGATCCTAATGTTGT
  CCCTCACTATGCAACTGGAGTGGTGCTGTTTGGTCTCTCGGCAGTGGTGGAGCTTCTAGG
  AGAGCCCTTTTGGGTCTTGGCACAAGCACATATGTTTGTGAAGCTCAAGGTGATTGCAGA
  GAGCCTGTCGGTAATTCTTAAGAGCGTTCTGACAGCTTTTCTCGTGCTGTGGTTTGCCTC
  ACTGGGGATTGTACATTTTTCTCTTTGGCCCAGCTTTTCTATACCAACAGTTCTGGTGCT
  CTGCTATGTATTTATTTCACAAAGTTACTGGGATGCCCCGAATCAACCAAGCTTTCAATC
  TCTTCCTGTCTTCCGATAACAGAATCTGTTACGCATCTTACATGAAATGACGGGTTCTAT
  TACCTGGCACAGAGGCAGACCTGACTGGACGCGTTCTA
• Restriction Sites: Please inquire
• ACCN: NM_052859
• ORF Size: 1626 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_052859.2, NP_443091.1
• RefSeq Size: 2810
• RefSeq ORF: 1626
• Locus ID: 91869
• Domains: Rft-1
• Protein Families: Transmembrane
• Protein Pathways: N-Glycan biosynthesis
• Gene Summary: This gene encodes an enzyme which catalyzes the translocation of the Man(5)GlcNAc (2)-PP-Dol intermediate from the cytoplasmic to the luminal side of the endoplasmic reticulum membrane in the pathway for the N-glycosylation of proteins. Mutations in this gene are associated with congenital disorder of glycosylation type In. [provided by RefSeq, Dec 2008]