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PI4KA (NM_058004) Human Untagged Clone
CAT#: SC120237
PI4KA (untagged)-Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 2
"NM_058004" in other vectors (2)
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Other products for "PI4KA"
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Symbol | PI4KA |
| Synonyms | PI4K-ALPHA; pi4K230; PIK4CA; PMGYCHA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_058004, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCGGCCCCGGCCCGGGGAGGCGGAGGCGGAGGCGGAGGCGGCGGCGGCTGCTCCGGCTCCGGCT CCAGCGCCTCGCGGGGCTTCTATTTCAACACGGTCCTGTCACTGGCCCGCTCCCTGGCGGTGCAGAGACC AGCATCCTTGGAGAAGGTCCAAAAGCTTCTTTGCATGTGTCCAGTGGATTTCCATGGGATCTTCCAGTTA GATGAAAGACGGAGAGATGCAGTGATTGCATTGGGCATTTTTCTGATTGAATCTGATCTTCAGCACAAAG ATTGTGTGGTTCCTTACCTTCTTCGACTTCTCAAAGGTCTTCCAAAAGTGTATTGGGTAGAAGAAAGCAC AGCTCGGAAAGGCAGAGGTGCCCTCCCGGTTGCAGAGAGCTTCAGCTTCTGCTTGGTAACTCTGCTGTCT GATGTGGCCTATAGGGATCCTTCACTTAGGGATGAGATTTTAGAGGTGCTTTTGCAGGTTTTGCATGTCC TCTTGGGGATGTGCCAGGCCTTGGAGATTCAAGACAAAGAATACCTTTGCAAGTATGCTATCCCATGCCT GATAGGAATCTCGCGAGCATTTGGGCGTTACAGCAACATGGAAGAGTCTCTCCTCTCAAAGCTCTTTCCC AAAATCCCTCCTCATTCCCTCCGTGTCCTGGAAGAGCTTGAAGGTGTTCGAAGGCGTTCCTTTAATGACT TCCGCTCCATCCTCCCCAGCAATCTGCTGACTGTCTGTCAGGAGGGTACCCTGAAGAGGAAAACCAGCAG TGTGTCCAGCATCTCTCAGGTCAGCCCTGAACGCGGCATGCCCCCTCCCAGTTCCCCTGGAGGATCTGCC TTTCACTACTTTGAAGCCTCCTGCTTGCCCGATGGGACTGCCCTAGAGCCTGAGTACTACTTTTCAACCA TCAGCTCCAGCTTCTCAGTCTCTCCCCTTTTCAACGGTGTCACATATAAGGAGTTTAACATTCCATTGGA AATGCTTCGGGAACTCTTAAACCTGGTGAAGAAGATCGTTGAGGAGGCTGTTCTCAAATCTTTGGATGCC ATTGTAGCCAGTGTGATGGAGGCCAACCCCAGTGCTGATCTCTACTACACTTCCTTCAGTGACCCTCTCT ACCTGACCATGTTCAAGATGCTGCGTGACACTCTGTACTACATGAAGGACCTCCCGACCTCTTTTGTGAA GGAGATCCATGATTTTGTGCTGGAGCAGTTCAACACGAGCCAGGGGGAGCTCCAGAAGATTCTACATGAC GCAGACCGGATCCACAATGAGCTGAGCCCCCTCAAACTGCGCTGTCAGGCGAATGCTGCCTGTGTGGACC TCATGGTGTGGGCTGTGAAGGACGAGCAGGGTGCAGAAAACCTTTGCATCAAGCTATCTGAGAAGCTGCA GTCCAAGACGTCCAGCAAAGTCATTATTGCTCACTTGCCCCTGCTGATCTGCTGTCTGCAGGGTTTGGGC CGCCTGTGCGAGAGGTTCCCGGTGGTGGTGCACTCTGTGACACCGTCCTTGCGAGACTTCCTGGTCATCC CGTCCCCAGTTCTGGTGAAGCTCTACAAGTACCACAGTCAGTACCACACAGTTGCTGGCAATGATATAAA AATCAGTGTGACCAATGAGCATTCCGAGTCAACCCTGAACGTCATGTCGGGTAAGAAGAGCCAGCCCTCC ATGTACGAGCAGCTCCGAGACATCGCTATTGACAACATCTGCAGGTGCCTGAAGGCTGGATTGACGGTGG ACCCAGTGATTGTGGAGGCGTTCTTGGCCAGCCTGTCCAACCGGCTCTACATCTCTCAGGAGAGCGACAA GGACGCTCACTTGATTCCCGACCACACAATCCGAGCCTTGGGACACATTGCGGTGGCCTTGAGGGACACC CCGAAGGTCATGGAGCCCATTCTGCAGATCCTACAGCAGAAATTCTGCCAGCCACCCTCCCCCCTCGATG TGCTGATTATTGACCAGCTGGGCTGCCTGGTTATCACCGGAAATCAATACATCTATCAGGAAGTGTGGAA CCTCTTCCAGCAGATCAGTGTGAAGGCCAGCTCCGTTGTATACTCAGCCACCAAAGATTACAAGGACCAC GGCTATAGGCATTGCTCCCTGGCAGTGATTAATGCCCTGGCCAACATCGCGGCCAACATCCAAGACGAGC ACCTGGTGGATGAGCTGCTCATGAACCTGTTGGAGTTGTTTGTGCAGCTGGGGCTGGAGGGGAAGCGAGC CAGCGAGAGGGCAAGCGAGAAGGGCCCTGCCCTAAAGGCTTCTAGCAGTGCAGGGAACTTGGGAGTACTC ATTCCTGTAATAGCTGTGCTCACCCGACGACTGCCACCCATCAAAGAAGCTAAGCCTCGGTTACAGAAGC TCTTCCGAGACTTCTGGCTGTATTCCGTTCTGATGGGATTCGCTGTGGAGGGCTCAGGACTCTGGCCAGA AGAATGGTACGAGGGGGTCTGTGAAATAGCCACTAAGTCCCCCTTGCTCACCTTTCCCAGCAAGGAGCCA CTGCGGTCCGTCCTCCAGTATAACTCAGCCATGAAGAATGACACGGTCACCCCCGCTGAGCTGAGTGAGC TCCGCAGCACTATCATCAACCTGCTGGACCCCCCTCCCGAGGTGTCCGCACTCATCAACAAGCTGGACTT CGCCATGTCCACCTACCTCCTCTCTGTGTACCGGCTGGAGTACATGAGGGTACTGCGTTCAACAGATCCT GATCGCTTCCAGGTAATGTTCTGCTACTTTGAGGATAAAGCTATTCAGAAAGACAAATCTGGGATGATGC AGTGTGTGATTGCAGTCGCGGACAAAGTATTCGATGCCTTCCTGAACATGATGGCGGATAAAGCCAAGAC CAAGGAGAACGAGGAGGAGCTGGAGCGGCACGCTCAGTTCCTGTTGGTGAACTTCAACCACATCCACAAG AGGATAAGGAGGGTGGCAGACAAGTATCTATCTGGTCTGGTGGATAAGTTTCCCCACTTGCTCTGGAGCG GGACTGTGCTGAAGACCATGCTGGACATCCTGCAGACCCTGTCACTGTCACTGAGCGCTGATATTCACAA GGATCAGCCTTACTATGACATCCCCGACGCCCCCTACCGGATCACGGTTCCTGACACGTACGAAGCCCGT GAGAGCATTGTGAAGGACTTCGCTGCACGCTGTGGGATGATCCTCCAGGAGGCCATGAAGTGGGCACCTA CCGTCACCAAGTCCCACCTGCAGGAATATCTGAACAAACATCAGAACTGGGTATCGGGACTGTCCCAGCA CACGGGGCTGGCCATGGCCACTGAGAGCATCCTTCACTTTGCTGGCTACAACAAGCAGAACACAACTCTT GGGGCAACTCAGCTGAGCGAGCGCCCGGCCTGTGTGAAGAAAGACTACTCCAACTTCATGGCATCCCTGA ATCTGCGCAACCGCTACGCGGGCGAGGTGTATGGAATGATTCGGTTCTCAGGCACCACAGGCCAGATGTC TGACCTGAACAAAATGATGGTCCAGGATCTACATTCAGCTTTAGACCGCAGTCATCCTCAGCACTACACG CAGGCCATGTTCAAGCTGACCGCAATGCTCATTAGCAGTAAAGATTGTGACCCGCAGCTCCTTCATCATC TGTGCTGGGGTCCCCTCCGGATGTTCAATGAGCATGGCATGGAGACGGCCCTGGCCTGCTGGGAGTGGCT GCTGGCTGGCAAGGATGGAGTGGAAGTGCCGTTCATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTG GAGCAGAAATTTGGCCTGTTTTCTGCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCTCGGAAGCAAGTC AACCCAAACCCTGTCCCCCCGAAGTGACCCCCCACTACATCTGGATCGACTTCCTGGTGCAGCGGTTTGA GATCGCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTCTCCAGCCTGCTGCAGCGCTCCATGTCCCTG AACATCGGCGGGGCCAAGGGGAGCATGAACCGGCACGTGGCGGCCATCGGGCCCCGCTTCAAGCTGCTGA CCCTGGGGCTGTCCCTCCTGCATGCCGATGTGGTTCCAAATGCAACCATCCGCAATGTGCTTCGCGAGAA GATCTACTCCACTGCCTTTGACTACTTCAGCTGTCCCCCAAAGTTCCCTACTCAAGGAGAGAAGCGGCTG CGTGAAGACATAAGCATCATGATTAAATTTTGGACCGCCATGTTCTCAGATAAGAAGTACCTGACCGCCA GCCAGCTTGTTCCCCCAGATAATCAGGACACCCGGAGCAACCTGGACATAACTGTCGGCTCTCGGCAACA AGCCACCCAAGGCTGGATCAACACATACCCCCTGTCCAGCGGCATGTCCACCATCTCCAAGAAATCAGGC ATGTCTAAGAAAACCAACCGGGGCTCCCAGCTGCACAAATACTACATGAAGCGCAGGACGCTGCTGCTGT CCCTGCTGGCCACTGAGATCGAGCGTCTCATCACATGGTACAACCCGCTGTCAGCCCCGGAACTGGAACT AGACCAGGCCGGAGAGAACAGCGTGGCCAACTGGAGATCTAAGTACATCAGCCTGAGTGAGAAGCAGTGG AAGGACAACGTGAACCTCGCCTGGAGCATCTCTCCCTACCTAGCCGTGCAGCTGCCTGCCAGGTTTAAGA ACACAGAAGCCATTGGGAACGAAGTGACCCGTCTCGTTCGGTTGGACCCGGGAGCCGTTAGTGATGTGCC TGAAGCAATCAAGTTCCTGGTCACCTGGCACACCATCGACGCCGATGCTCCAGAGCTCAGCCATGTGCTG TGCTGGGCGCCCACGGACCCACCCACAGGCCTCTCCTACTTCTCCAGCATGTACCCGCCGCACCCTCTCA CGGCGCAGTACGGGGTGAAAGTCCTGCGGTCCTTCCCTCCGGACGCCATCCTCTTCTACATCCCCCAGAT TGTGCAGGCCCTCAGGTACGACAAGATGGGCTATGTGCGGGAGTATATTCTGTGGGCAGCGTCTAAATCC CAGCTTCTGGCACACCAGTTCATCTGGAACATGAAGACTAACATTTATCTAGATGAAGAGGGCCACCAGA AAGACCCTGACATCGGCGACCTCCTGGATCAGTTGGTAGAGGAGATCACAGGCTCCTTGTCCGGCCCAGC GAAGGACTTTTACCAGCGGGAGTTTGATTTCTTTAACAAGATCACCAACGTGTCGGCTATCATCAAGCCC TACCCTAAAGGCGACGAGAGAAAGAAGGCTTGTCTGTCGGCCCTGTCTGAAGTGAAGGTGCAGCCGGGCT GCTACCTGCCCAGCAACCCTGAGGCCATTGTGCTGGACATCGACTACAAGTCTGGGACCCCGATGCAGAG TGCTGCAAAAGCCCCATATCTGGCCAAGTTCAAGGTGAAGCGATGTGGAGTTAGTGAACTTGAAAAAGAA GGTCTGCGGTGCCGCTCAGACTCCGAGGATGAGTGCAGCACGCAGGAGGCCGACGGCCAGAAGATCTCCT GGCAGGCAGCCATCTTCAAGGTGGGAGACGACTGCCGGCAGGACATGCTGGCCCTGCAGATCATCGACCT CTTCAAGAACATCTTCCAGCTGGTCGGCCTGGACCTCTTTGTTTTTCCCTACCGCGTGGTGGCCACTGCC CCTGGGTGCGGGGTGATCGAGTGCATCCCCGACTGCACCTCCCGGGACCAGCTGGGCCGCCAGACAGACT TCGGCATGTACGACTACTTCACACGCCAGTACGGGGATGAGTCCACTCTGGCCTTCCAGCAGGCCCGCTA CAACTTCATCCGAAGCATGGCCGCCTACAGCCTCCTGCTGTTCCTGCTGCAGATCAAGGACAGACACAAC GGCAACATTATGCTGGACAAGAAGGGTCATATCATCCACATCGACTTTGGCTTCATGTTTGAAAGCTCGC CGGGCGGCAATCTCGGCTGGGAACCCGACATCAAGCTGACGGATGAGATGGTGATGATCATGGGGGGCAA GATGGAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTAC ATGGACGCGGTCGTCTCCCTGGTCACTCTCATGTTGGACACGGGCCTGCCCTGTTTTCGCGGCCAGACAA TCAAGCTCTTGAAGCACAGGTTTAGCCCCAACATGACTGAGCGCGAGGCTGCAAATTTCATCATGAAGGT CATCCAGAGCTGCTTCCTCAGCAACAGGAGCCGGACCTACGACATGATCCAGTACTATCAGAATGACATC CCCTACTGA >OriGene 5' read for NM_058004 unedited
GCCTTTCAGATTTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGCCTCG TGCCGAATTCGGCACGAGGCACGGTCCTGTCACTGGCCCGCTCCCTGGCGGTGCAGAGAC CAGCATCCTTGGAGAAGGTCCAAAAGCTTCTTTGCATGTGTCCAGTGGATTTCCATGGGA TCTTCCAGTTAGATGAAAGACGGAGAGATGCAGTGATTGCATTGGGCATTTTTCTGATTG AATCTGATCTTCAGCACAAAGATTGTGTGGTTCCTTACCTTCTTCGACTTCTCAAAGGTC TTCCAAAAGTGTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGGTGCCCTCCCGG TTGCAGAGAGCTTCAGCTTCTGCTTGGTAACTCTGCTGTCTGATGTGGCCTATAGGGATC CTTCACTTAGGGATGAGATTTTAGAGGTGCTTTTGCAGGTTTTGCATGTCCTCTTGGGGA TGTGCCAGGCCTTGGAGATTCAAGACAAAGAATACCTTTGCAAGTATGCTATCCCATGCC TGATAGGAATCTCGCGAGCATTTGGGCGTTACAGCAACATGGAAGAGTCTCTCCTCTCAA AGCTCTTTCCCAAAATCCCTCCTCATTCCCTCCGTGTCCTGGAAGAGCTTGAAGGTGTTC GAAGGCGTTCCTTTAATGACTTCCGCTCCATCCTCCCCAGCAATCTGCTGACTGTCTGTC AGGAGGGTACCCTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAAGTCAGCCCTG AACGCNGCATGCCCCCTTCCAGTTCCCCTGGAGGATCTGCCTTTCACTACTTTGAAGCCT NCTGTTTGCCCGATGGGACTGCCCTAGAGCCTGAGTACTACTTTTTCACCATCAGCTNCA GCTTCTCAAGTCTCTCCCTTTTTCAACGTG >OriGene 3' read for NM_058004 unedited
NNNGGGAAATCTATGTACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTCACTATTTTGGGTTTTTATTTTGTTGATGTTGGTTAAAT CTTATCTCTTTTTTTATACACAATACTTCATGTCCCTATGAAATAAAACAGGTAGGGAAT ATGTCCAGTGCAAACAGAGGACTCACACCTGTGCATAGACAGCACCATCCATTGATTGTC GCTGCAGTCCATGGCGTTACCAAGGCTGCCCCACCCACGTGCTGCCCCAGGAGGCGCTAC CAGGTTCTTTGGGCCACAGGCCTCTCCTCCACTGCATGTGGCGGCAGGGCAGGGAGGTCC CAGGGCTCCATGATTGTGGGACAGCTTTGAGGGCACATGGGGCAAAGGCCCTCGAAGGTC CCCTCCTCAGTAGGGGATGTCATTCTGATAGTACTGGATCATGTCGTAGGTCCGGCTCCT GTTGCTGAGGAAGCACCTCTGGATGACCTTCATGATGAAATTTGCAGCCTCGCGCTCAGT CATGTTGGGGCTAAACCTGTGCTTCAAAAGCTTGATTGTCTGGCCGCAAAAACAGGGCAG GCCCGTGTCCAACATGAAAGTGACCAGGGAGACGACCGCGTCCATGTAGGGCCGCACAGC CAGGTATCCTCGGGAACACATCTCCCTGAACCACTTGAGAGGTGTGGCCTCCATCTTGCC CCCCATGATCATCACCATTTTATCCGTCAGCTTGATGTCGGGTTCCCAACCCAAAATGCC CCCCGGCGAGCTTTCAAACATGAAACCCAAATTCCAGTGGGAAGAAAAGGCCCTTCTTGG TCCACAAAAGGGTGGCCTTTTGTTCTTCCCTGATTCGCAAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_058004 |
| Insert Size | 7000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_058004.1, NP_477352.1 |
| RefSeq Size | 6768 bp |
| RefSeq ORF | 6768 bp |
| Locus ID | 5297 |
| Cytogenetics | 22q11.21 |
| Domains | PI3_PI4_kinase, PI3Ka |
| Protein Families | Druggable Genome |
| Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | 'This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2018]' |
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