CD97 (ADGRE5) (NM_078481) Human Untagged Clone

CAT#: SC120259

CD97 (untagged)-Human CD97 molecule (CD97), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADGRE5
• Synonyms: CD97; TM7LN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_078481, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCA
  GGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAATCCAGG
  GTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTTGTGACGACATCAACGAGTGTGCAACA
  CCGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCA
  GCCCGGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGT
  GGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTTGGCAGCTAT
  ACCTGCCAGTGCCTGCCTGGCTTCAAGTTCATACCTGAGGATCCGAAGGTCTGCACAGATGTGAATGAAT
  GCACCTCCGGACAAAACCCGTGCCACAGCTCCACCCACTGCCTCAACAACGTGGGCAGCTATCAGTGCCG
  CTGCCGCCCGGGCTGGCAACCGATTCCGGGGTCCCCCAATGGCCCAAACAATACCGTCTGTGAAGATGTG
  GACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACA
  GCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTGA
  AGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTCGAC
  AAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCAGCCGAGGTCACCATCCAGAATGTCATCAAAT
  TGGTGGATGAACTGATGGAAGCTCCTGGAGACGTAGAGGCCCTGGCGCCACCTGTCCGGCACCTCATAGC
  CACCCAGCTGCTCTCAAACCTTGAAGATATCATGAGGATCCTGGCCAAGAGCCTGCCTAAAGGCCCCTTC
  ACCTACATTTCCCCTTCGAACACAGAGCTGACCCTGATGATCCAGGAGCGGGGGGACAAGAACGTCACTA
  TGGGTCAGAGCAGCGCACGCATGAAGCTGAATTGGGCTGTGGCAGCTGGAGCCGAGGATCCAGGCCCCGC
  CGTGGCGGGCATCCTCTCCATCCAGAACATGACGACATTGCTGGCCAATGCCTCCTTGAACCTGCATTCC
  AAGAAGCAAGCCGAACTGGAGGAGATATATGAAAGCAGCATCCGTGGTGTCCAACTCAGACGCCTCTCTG
  CCGTCAACTCCATCTTTCTGAGCCACAACAACACCAAGGAACTCAACTCCCCCATCCTTTTCGCCTTCTC
  CCACCTTGAGTCCTCCGATGGGGAGGCGGGAAGAGACCCTCCTGCCAAGGACGTGATGCCTGGGCCACGG
  CAGGAGCTGCTCTGTGCCTTCTGGAAGAGTGACAGCGACAGGGGAGGGCACTGGGCCACCGAGGGCTGCC
  AGGTGCTGGGCAGCAAGAACGGCAGCACCACCTGCCAATGCAGCCACCTGAGCAGCTTTGCGATCCTTAT
  GGCTCATTATGACGTGGAGGACTGGAAGCTGACCCTGATCACCAGGGTGGGACTGGCGCTGTCACTCTTC
  TGCCTGCTGCTGTGCATCCTCACTTTCCTGCTGGTGCGGCCCATCCAGGGCTCGCGCACCACCATACACC
  TGCACCTCTGCATCTGCCTCTTCGTGGGCTCCACCATCTTCCTGGCCGGCATCGAGAACGAAGGCGGCCA
  GGTGGGGCTGCGCTGCCGCCTGGTGGCCGGGCTGCTGCACTACTGTTTCCTGGCCGCCTTCTGCTGGATG
  AGCCTCGAAGGCCTGGAGCTCTACTTTCTTGTGGTGCGCGTGTTCCAAGGCCAGGGCCTGAGTACGCGCT
  GGCTCTGCCTGATCGGCTATGGCGTGCCCCTGCTCATCGTGGGCGTCTCGGCTGCCATCTACAGCAAGGG
  CTACGGCCGCCCCAGATACTGCTGGTTGGACTTTGAGCAGGGCTTCCTCTGGAGCTTCTTGGGACCTGTG
  ACCTTCATCATTTTGTGCAATGCTGTCATTTTCGTGACTACCGTCTGGAAGCTCACTCAGAAGTTTTCTG
  AAATCAATCCAGACATGAAGAAATTAAAGAAGGCGAGGGCGCTGACCATCACGGCCATCGCGCAGCTCTT
  CCTGTTGGGCTGCACCTGGGTCTTTGGCCTGTTCATCTTCGACGATCGGAGCTTGGTGCTGACCTATGTG
  TTTACCATCCTCAACTGCCTGCAGGGCGCCTTCCTCTACCTGCTGCACTGCCTGCTCAACAAGAAGGTTC
  GGGAAGAATACCGGAAGTGGGCCTGCCTAGTTGCTGGGGGGAGCAAGTACTCAGAATTCACCTCCACCAC
  GTCTGGCACTGGCCACAATCAGACCCGGGCCCTCAGGGCATCAGAGTCCGGCATATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_078481 unedited
  GGGGCCCGGGGGAAGNNGCAAATTCCCNNNGGNGTTCGGAATTGAAACCCATTTATATAG
  GCGGACGCGCAATTCGCACCANAACGCCAGTTCCGGGGAGGGCCCTGGGCCAGCGGCTGT
  CCGCCCCCCCTCCTTCATAAAGTCCTGGCCTCGGGACAGCCTGCACAGCTGCCTAGCCTG
  TGGAGACGGGACAGCCCTGTCCCACTCACTCTTTCCCCTGCCGCTCCTGCCGGCAGCTCC
  AACCATGGTAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGA
  AACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGC
  CACCGCCTGTCGCTGCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGAC
  GGAGACTTGTGACGACATCAACGAGTGTGCAACACCGTCGAAAGTGTCATGCGGAAAATT
  CTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCC
  TGTNTCTGGNGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGA
  ATGTCAGCAGAACCCAAGGCTCTGNTAAAGCTACGGCACCTGCGTCAACACCCTTGGCAG
  CTATACCTGCCAGTGCCTGCCTGGCTTAAAGTCATACCTGAGGATCCGAAGGTCTGCACA
  GATGTGAATGAATGCACCTNCGGGACANAACCCCGTGCACAGNNTCCACCCACTGCCTCA
  ACAACGTGGGGCAGCTATCANTGCCGCTGCCNGCCGGCCTGGCANCCCGATCCCGGGGTC
  CCCACATGGCCCCAACAATACCCTTTGTGAAGAATGTGGACAATGCANNTCCGGGCAGCA
  TAAGTGTGACAGGTCCACCGTTTGGTTAAAACCCGGGGTTATACAGCTGCCCGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_078481 unedited
  ACAAACTATGGTCGTTATCCCTCTTCTAGATTTGTTTTTTTTTTTTTTTTTTTTTTTTTT
  TGGATGCATGTGTTTAATTTTAAGTGTCAACACTGGAAAAATTTTAACAGATAAAAACAC
  ATTACACAACGTCTACAACCTTCACAACAGTGAGAACCTCCTGCCCGGCCAGGCAGGGCA
  GGCCTCTGTACCGTGAGCCTCAAGCAGTGGCCCCAAGGGGCCTCGCCATGGGGCCTCTGA
  CATCAGTCTTAGCTTAAGAGGGAAGCTGAGCCCCAGCGCCTAGTCTCTTCAGTTCTGCAG
  CAAAGACATGAGAGGAAAAGTCCAGGATGGGACAAGCGCCCTTAGTCTGTGCCCGGAAGN
  TTNAACCTAGGGGGCCCCACCAGAGGTTGCCCCAGGGGGGCAAAACATTGGCTTGGCATT
  TGCCAAGCCCCCTGGGGCCCCAACCCCCCTTGTTCCCCCACCCCTGGGGGTCCACCAAAG
  GTGGAAACCANAAGAAGGCAGGCCCAGCAAGGCAGGAACCCGGTGGGGTTTCCGAATTTC
  CACCCCCACTTGGGGGTGGTCCCCGGGAACTTTTTGGGTGGCCCACGAACTATTAGGTTG
  GGCCATTCCCCTTCCCTTGGGGGGGCCCCCCGGGGTTTTCAAAGGAAAAAGGAAAGGTTG
  GGGAAGGGGGTTTTTTAAAATTGGGGGGGGGGGGACCCCAAAAAAGGGGGNNGGGNNNTT
  NNNNNNNNNNNNCNCTTTTTTTTTGTTTCCNNNNAAAAAAAAAANNNCCTNNTTTTGTGG
  GGGCTTCTCCCNNNNNNTNNNNTCCCCCTTNGGNGGTNNCCCCCCCCCCCCCCNNNNNTT
  TCCCTTTTTGGGGTGTTGTTTGTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_078481
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_078481.2, NP_510966.1
• RefSeq Size: 3247 bp
• RefSeq ORF: 2508 bp
• Locus ID: 976
• Cytogenetics: 19p13.12
• Domains: GPS, 7tm_2, EGF_CA, EGF, EGF
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, GPCR, Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as CD97(EGF 1,2,3,4,5)).