CD97 (ADGRE5) (NM_078481) Human Untagged Clone

CAT#: SC120259

CD97 (untagged)-Human CD97 molecule (CD97), transcript variant 1


  "NM_078481" in other vectors (6)

Reconstitution Protocol

USD 1,690.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ADGRE5
Synonyms CD97; TM7LN1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_078481, the custom clone sequence may differ by one or more nucleotides


ATGGGAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCA
GGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAATCCAGG
GTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTTGTGACGACATCAACGAGTGTGCAACA
CCGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCA
GCCCGGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGT
GGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTTGGCAGCTAT
ACCTGCCAGTGCCTGCCTGGCTTCAAGTTCATACCTGAGGATCCGAAGGTCTGCACAGATGTGAATGAAT
GCACCTCCGGACAAAACCCGTGCCACAGCTCCACCCACTGCCTCAACAACGTGGGCAGCTATCAGTGCCG
CTGCCGCCCGGGCTGGCAACCGATTCCGGGGTCCCCCAATGGCCCAAACAATACCGTCTGTGAAGATGTG
GACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACA
GCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTGA
AGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTCGAC
AAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCAGCCGAGGTCACCATCCAGAATGTCATCAAAT
TGGTGGATGAACTGATGGAAGCTCCTGGAGACGTAGAGGCCCTGGCGCCACCTGTCCGGCACCTCATAGC
CACCCAGCTGCTCTCAAACCTTGAAGATATCATGAGGATCCTGGCCAAGAGCCTGCCTAAAGGCCCCTTC
ACCTACATTTCCCCTTCGAACACAGAGCTGACCCTGATGATCCAGGAGCGGGGGGACAAGAACGTCACTA
TGGGTCAGAGCAGCGCACGCATGAAGCTGAATTGGGCTGTGGCAGCTGGAGCCGAGGATCCAGGCCCCGC
CGTGGCGGGCATCCTCTCCATCCAGAACATGACGACATTGCTGGCCAATGCCTCCTTGAACCTGCATTCC
AAGAAGCAAGCCGAACTGGAGGAGATATATGAAAGCAGCATCCGTGGTGTCCAACTCAGACGCCTCTCTG
CCGTCAACTCCATCTTTCTGAGCCACAACAACACCAAGGAACTCAACTCCCCCATCCTTTTCGCCTTCTC
CCACCTTGAGTCCTCCGATGGGGAGGCGGGAAGAGACCCTCCTGCCAAGGACGTGATGCCTGGGCCACGG
CAGGAGCTGCTCTGTGCCTTCTGGAAGAGTGACAGCGACAGGGGAGGGCACTGGGCCACCGAGGGCTGCC
AGGTGCTGGGCAGCAAGAACGGCAGCACCACCTGCCAATGCAGCCACCTGAGCAGCTTTGCGATCCTTAT
GGCTCATTATGACGTGGAGGACTGGAAGCTGACCCTGATCACCAGGGTGGGACTGGCGCTGTCACTCTTC
TGCCTGCTGCTGTGCATCCTCACTTTCCTGCTGGTGCGGCCCATCCAGGGCTCGCGCACCACCATACACC
TGCACCTCTGCATCTGCCTCTTCGTGGGCTCCACCATCTTCCTGGCCGGCATCGAGAACGAAGGCGGCCA
GGTGGGGCTGCGCTGCCGCCTGGTGGCCGGGCTGCTGCACTACTGTTTCCTGGCCGCCTTCTGCTGGATG
AGCCTCGAAGGCCTGGAGCTCTACTTTCTTGTGGTGCGCGTGTTCCAAGGCCAGGGCCTGAGTACGCGCT
GGCTCTGCCTGATCGGCTATGGCGTGCCCCTGCTCATCGTGGGCGTCTCGGCTGCCATCTACAGCAAGGG
CTACGGCCGCCCCAGATACTGCTGGTTGGACTTTGAGCAGGGCTTCCTCTGGAGCTTCTTGGGACCTGTG
ACCTTCATCATTTTGTGCAATGCTGTCATTTTCGTGACTACCGTCTGGAAGCTCACTCAGAAGTTTTCTG
AAATCAATCCAGACATGAAGAAATTAAAGAAGGCGAGGGCGCTGACCATCACGGCCATCGCGCAGCTCTT
CCTGTTGGGCTGCACCTGGGTCTTTGGCCTGTTCATCTTCGACGATCGGAGCTTGGTGCTGACCTATGTG
TTTACCATCCTCAACTGCCTGCAGGGCGCCTTCCTCTACCTGCTGCACTGCCTGCTCAACAAGAAGGTTC
GGGAAGAATACCGGAAGTGGGCCTGCCTAGTTGCTGGGGGGAGCAAGTACTCAGAATTCACCTCCACCAC
GTCTGGCACTGGCCACAATCAGACCCGGGCCCTCAGGGCATCAGAGTCCGGCATATGA


>OriGene 5' read for NM_078481 unedited
GGGGCCCGGGGGAAGNNGCAAATTCCCNNNGGNGTTCGGAATTGAAACCCATTTATATAG
GCGGACGCGCAATTCGCACCANAACGCCAGTTCCGGGGAGGGCCCTGGGCCAGCGGCTGT
CCGCCCCCCCTCCTTCATAAAGTCCTGGCCTCGGGACAGCCTGCACAGCTGCCTAGCCTG
TGGAGACGGGACAGCCCTGTCCCACTCACTCTTTCCCCTGCCGCTCCTGCCGGCAGCTCC
AACCATGGTAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGA
AACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGC
CACCGCCTGTCGCTGCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGAC
GGAGACTTGTGACGACATCAACGAGTGTGCAACACCGTCGAAAGTGTCATGCGGAAAATT
CTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCC
TGTNTCTGGNGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGA
ATGTCAGCAGAACCCAAGGCTCTGNTAAAGCTACGGCACCTGCGTCAACACCCTTGGCAG
CTATACCTGCCAGTGCCTGCCTGGCTTAAAGTCATACCTGAGGATCCGAAGGTCTGCACA
GATGTGAATGAATGCACCTNCGGGACANAACCCCGTGCACAGNNTCCACCCACTGCCTCA
ACAACGTGGGGCAGCTATCANTGCCGCTGCCNGCCGGCCTGGCANCCCGATCCCGGGGTC
CCCACATGGCCCCAACAATACCCTTTGTGAAGAATGTGGACAATGCANNTCCGGGCAGCA
TAAGTGTGACAGGTCCACCGTTTGGTTAAAACCCGGGGTTATACAGCTGCCCGTGG
>OriGene 3' read for NM_078481 unedited
ACAAACTATGGTCGTTATCCCTCTTCTAGATTTGTTTTTTTTTTTTTTTTTTTTTTTTTT
TGGATGCATGTGTTTAATTTTAAGTGTCAACACTGGAAAAATTTTAACAGATAAAAACAC
ATTACACAACGTCTACAACCTTCACAACAGTGAGAACCTCCTGCCCGGCCAGGCAGGGCA
GGCCTCTGTACCGTGAGCCTCAAGCAGTGGCCCCAAGGGGCCTCGCCATGGGGCCTCTGA
CATCAGTCTTAGCTTAAGAGGGAAGCTGAGCCCCAGCGCCTAGTCTCTTCAGTTCTGCAG
CAAAGACATGAGAGGAAAAGTCCAGGATGGGACAAGCGCCCTTAGTCTGTGCCCGGAAGN
TTNAACCTAGGGGGCCCCACCAGAGGTTGCCCCAGGGGGGCAAAACATTGGCTTGGCATT
TGCCAAGCCCCCTGGGGCCCCAACCCCCCTTGTTCCCCCACCCCTGGGGGTCCACCAAAG
GTGGAAACCANAAGAAGGCAGGCCCAGCAAGGCAGGAACCCGGTGGGGTTTCCGAATTTC
CACCCCCACTTGGGGGTGGTCCCCGGGAACTTTTTGGGTGGCCCACGAACTATTAGGTTG
GGCCATTCCCCTTCCCTTGGGGGGGCCCCCCGGGGTTTTCAAAGGAAAAAGGAAAGGTTG
GGGAAGGGGGTTTTTTAAAATTGGGGGGGGGGGGACCCCAAAAAAGGGGGNNGGGNNNTT
NNNNNNNNNNNNCNCTTTTTTTTTGTTTCCNNNNAAAAAAAAAANNNCCTNNTTTTGTGG
GGGCTTCTCCCNNNNNNTNNNNTCCCCCTTNGGNGGTNNCCCCCCCCCCCCCCNNNNNTT
TCCCTTTTTGGGGTGTTGTTTGTCA
Restriction Sites NotI-NotI     
ACCN NM_078481
Insert Size 3500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_078481.2, NP_510966.1
RefSeq Size 3247 bp
RefSeq ORF 2508 bp
Locus ID 976
Cytogenetics 19p13.12
Domains GPS, 7tm_2, EGF_CA, EGF, EGF
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, GPCR, Secreted Protein, Transmembrane
Gene Summary 'This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as CD97(EGF 1,2,3,4,5)).

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