myosin light chain 1 (MYL1) (NM_079422) Human Untagged Clone

CAT#: SC120265

MYL1 (untagged)-Human myosin, light chain 1, alkali, skeletal, fast (MYL1), transcript variant 3f

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL1
• Synonyms: MLC1F; MLC3F; MYOFTA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_079422, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCTTCAGTGCTGACCAGATTGCTGAATTCAAGGAGGCATTTCTCCTGTTTGACAGAACAGGTGATT
  CCAAGATCACCTTAAGCCAGGTCGGTGATGTCCTTCGAGCTCTGGGCACAAATCCCACCAATGCAGAGGT
  CAGGAAAGTTCTGGGAAACCCCAGCAATGAAGAGCTGAATGCCAAGAAAATTGAGTTTGAACAATTTCTG
  CCTATGATGCAAGCCATTTCCAACAACAAGGACCAGGCCACCTATGAAGACTTTGTTGAGGGTCTGCGTG
  TCTTTGACAAGGAAGGCAATGGCACAGTCATGGGTGCTGAACTCCGCCATGTTCTAGCCACCCTGGGTGA
  AAAGATGAAAGAGGAAGAAGTGGAAGCCCTGATGGCAGGTCAAGAAGACTCCAATGGCTGCATCAACTAC
  GAAGCTTTTGTCAAGCACATCATGTCTATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_079422 unedited
  NGGGGAGTCGACTTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAGCA
  GCCCTCAACTCACTCTTCAGCTTCCCTGCTGTGTTGCAGCCCAGCCGCTCCATCATGTCC
  TTCAGTGCTGACCAGATTGCTGAATTCAAGGAGGCATTTCTCCTGTTTGACAGAACAGGT
  GATTCCAAGATCACCTTAAGCCAGGTCGGTGATGTCCTTCGAGCTCTGGGCACAAATCCC
  ACCAATGCAGAGGTCAGGAAAGTTCTGGGAAACCCCAGCAATGAAGAGCTGAATGCCAAG
  AAAATTGAGTTTGAACAATTTCTGCCTATGATGCAAGCCATTTCCAACAACAAGGACCAG
  GCCACCTATGAAGACTTTGTTGAGGGTCTGCGTGTCTTTGACAAGGAAGGCAATGGCACA
  GTCATGGGTGCTGAACTCCGCCATGTTCTAGCCACCCTGGGTGAAAAGATGAAAGAGGAA
  GAAGTGGAAGCCCTGATGGCAGGTCAAGAAGACTCCAATGGCTGCATCAACTACGAAGCT
  TTTGTCAAGCACATCATGTCTATCTGAATGGAGCTCTCAAGAACAAGCATTGTTTAGGAA
  GACTGGCTGGAAACTTATTTTAATCACACCCATGACAAACTCTCCAGATCTGTTTACCAT
  CATTCAGGAAAACAAAGCAATCTGGACGGTTCAAGACTGAGCAACTCCCTGAATTTTTAT
  ACATCTTCAGTTTTTCTCTGAATTGAATTCATACCACACAAACAAAGTGTCTGCTGCTCT
  AGATGAGAAAGATAAAATATTGACAATCTCAAATCCAGCACCATTCTTTATTATCTACCA
  TGAATCAACAAACATTCTTAAAACAAATAATCAATAAACAATTNTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_079422 unedited
  ACTATGNACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTCCCAGACTGACCAAAATTGTTTATTGATTTATTGTTTTAAAA
  ATGTTTGTTGATTCATGGTAAATAATAAAAAATGGGGCTTGGATTTGAAATTGCCAATAT
  TTTATTCTTCTCATCTAGAGCAGCAAACACTTTGTTTGGGGGGTATGAATTCAATTCAGA
  GAAAAACTGAAGATGTTTAAAAATTCAGGGAGTTGCTCAGTCTTGAACCGTCCAAATTGC
  TTTGTTTTCCTGAATGATGGGAAACAAATCTGGAGAGTTTGTCATGGGTGTGATTAAAAT
  AAGTTTCCAGCCCGTCTTCCTAAACAATGCTTGTTCTTGAGAGCTCCATTCAAATAGACA
  TGATGTGCTTGACAAAAGCTTCGTAGTTGATGCCCCCCTTGGAGTCTTCTTGACCTGCCA
  TCAGGGCTTCCACTTCTTCCTCTTTCATCTTTTCACCCCGGGGGGCTAGAACATGGCGGA
  GTTCAGCACCCATGACTGTGCCATTGCCTTCCTTGGCAAAAACACGCAGACCCTCAACAA
  AGTCTTCATAGGTGGCCTGGTCCTTGTTGTTGGAAATGGCTTGCTCATAGGCCGAAATTG
  TTCAAACTCAATTTTCTTGGCATTCAGCTCTTCATTGCTGGGGGTTCCCAGAACTTTCCT
  GACCTCTGCATTGGTGGGATTTGTGCCCCCAGCTCGAAGGACATCCCCACCTGGCTTAAG
  GGATCTTGGAATCACCTGTTCTGTTCAACAGAAGAATGCCTCCTTGAATTTCCCAATCTG
  GTCCCCCCTTAAGACATGAATGGAGCGCCTGGGCTGCACACCCCCGGGAAACTCANAAAG
  GAGTTGAAGCTGCTCTCCTGCCCATATCCGGCCGCCCTTGTGGGGCTATACAAAATTGCC
  GGTCCCTAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_079422
• Insert Size: 900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_079422.1, NP_524146.1
• RefSeq Size: 853 bp
• RefSeq ORF: 453 bp
• Locus ID: 4632
• Cytogenetics: 2q34
• Domains: EFh
• Gene Summary: 'Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3f) encodes a protein that is 44 aa shorter than isoform 1f. Transcript variants 3f and 1f differ in their first two exons.