RAD17 (NM_133338) Human Untagged Clone

CAT#: SC120501

RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD17
• Synonyms: CCYC; HRAD17; R24L; RAD17SP; RAD24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_133338, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCAGGTAACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGCGTCTCTACTA
  TTACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACATTAGAAAG
  CAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCTTCCTTAGAACAGATTTATGGTTTAGAAAATTCA
  AAAGAATATCTGTCTGAAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCAGCATGAACTTGCTG
  TGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCAAAACA
  GGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATACTATCA
  AAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAAAGATGATTTCA
  AGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTCTCAGATAGCAGTTTTCAAAGA
  GTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGATGATCTGAGAACTGATAAGAAG
  ATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGATTCTCATACTTTACATGAAGTTCTAAGGA
  AGTATGTGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACAGTCTCAGTGGAGATAATAATCA
  AAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAATATTAGTTTCAACCCTGTGGCA
  CCAACAATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAGCTAACAAGAATGGAGGAAAAATTA
  CTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTGGTGATATCAGAAGTGCAATAAA
  CAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAGGAAAAAAGGAATGTCTTTAAAA
  TCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCTGATAGGGTTTTTGAAAATCAAGAGGTCC
  AAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTTCAGAGCTTTGGGGAAAATTCTATATTGTAAAAG
  AGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCCTCTCATTTATCAGAATATGAACGGGATACATTA
  CTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACATGCCTGGAGACTTATTTAATTTATATCTTCACC
  AAAACTACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCAGTGAATTTCTGAGTTTTGCAGA
  TATCCTCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAGCACATCTATAGCTACGAGAGGT
  GTGATGCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAAGGAGGAGGATCAAGTTTTCGACCCTTGC
  ACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCGGGAAAATTGCCTGGCAGCAAAAGCACTTTTTCC
  TGACTTCTGCCTACCAGCTTTATGCCTCCAAACTCAGCTATTGCCATACCTTGCTCTACTAACCATTCCA
  ATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATATTGGAAGGCTCCCTCTGAAGCGACACTTTGGAA
  GATTGAAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATAGACCCTGACAGCGGAGATGAAGCCCA
  GCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCCACTCAAGCCACTGTGCCGGAAACCTGG
  TCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCTAGCCAGCCCCAGCCCTTTTCAGCCCAAG
  GAGACATGGAAGAAAACATAATAATAGAAGACTACGAGAGTGATGGGACATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133338 unedited
  GTAGTATATAGGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGACGAA
  ATGAATCAGGTAACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGC
  GTCTCTACTATTACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAAT
  GGGCCTTCTACATTAGAAAGCAGCAGATTTCCAGCGTAGAAAAAGAGGAAATCTATCTTC
  CTTAGAACAGATTTATGGTTTAGAAAATTCAAAAGAATATCTGTCTGAAAATGAACCATG
  GGTGGATAAATATAAACCAGAAACTCAGCATGAACTTGCTGTGCATAAAAAGAAAATTGA
  AGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCAAAACAGGGTGGATC
  TATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATACTATC
  AAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAA
  AGATGATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTC
  TCAGATAGCAGTTTTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAAT
  GCTTGGAGATGATCTGAGAACTGATAAGAAAGATATTCTGGTTGAAGATTACCTAACCAG
  TTNTATCGGGATTCTCATACTTTACATGAAGTTCTAAGGAAGTATGTGNAGATTGGTCGA
  TGTCCTCTTNATATTATAATCTCCGACAGTCTCAGTGGAGATATAATCAAAGGTATTGTT
  TCCAAAGAAAATCAGAAGATGTCTATCTCAAATATAGTTTCACCCTGTGCACCNACATAT
  GATGAAATNNCTATCGATATGACTATGAGCTACAGATGGAGAAAATTCTGTCCTGCAAAC
  T
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_133338 unedited
  NGTATTCTATGNACCGCGCCGCTTTTNANGANCGNATTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTAAATTCAACATTTTAATTGGGATATAACTTATGTAAATACTTTCCA
  AAAATATCTAAAATATTCCACTGTACAACACATCAAAACTTTTTGAAAAGCGGGAGACAA
  TAAAGTGTAAATAAGGCACACAGTTTACACTCTTAATTGCATCCTAAACTGTTGAATAGT
  TGATATTTACAACCCCCCAATAACGTTTTTGCATCCTGAATTAATCCTTCACATCTTGAT
  ATTTGGCATAATGTTCAAACACACTTTATACACCCTGACCGCTCCTTAAACCACTCACAG
  ATTTAATAAATCCCGAAAAAATTAAAAAATTTGAAGGCTTGATCTTCTATTTACTTCAAA
  ATGACAAAAAAGTAGGTTTCTTGTGATGAAATACTCCCAGAATGAAGAATTAACAAACTG
  TTGTGTAATTCATCAAAAAAGCATATTAACTCTGCTGAAGTACCACTGAATGAAACAAAA
  ATGAAGCTGTGAAGTACCAATCTGATTAGCAGGCTGGCTTCTATGTCCCATCACTCTCGA
  AGCCTTCTATTATTATGTTTTCTTCCATGTCTCCTTGGGCTGAAAAGGGCTGGGGCTGGC
  TAGCAGGCAGTTCACTGGCACTATTCTGACTCAAAGGAAGAGACCAGGTTTCCGGCACAG
  TGGCTTGAGTGGGTTCACCCAGAGATTCCTCTGCAGAATGTCCTCCATTAAGCTGGGCTT
  CATCTCCGCTGTCAGGGTCTATCATTCCATGTTCCCTGTCAGTCAGGGCTTCCATTTTCA
  ATCTTCCAAAGTGTCGCTTCAGAGGAGGCTTCCAATATCTTGGATAAAAGAAATCTGACC
  TTGAATTCTCATTGGAATGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_133338
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133338.1, NP_579916.1
• RefSeq Size: 3076 bp
• RefSeq ORF: 2013 bp
• Locus ID: 5884
• Cytogenetics: 5q13.2
• Domains: Rad17
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (1) encodes the predominant isoform (1). Variants 1, 5, 6, 7, 8 and 9 encode the same isoform.