Decorin (DCN) (NM_133506) Human Untagged Clone

CAT#: SC120507

DCN (untagged)-Human decorin (DCN), transcript variant D

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCN
• Synonyms: CSCD; DSPG2; PG40; PGII; PGS2; SLRR1B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_133506, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGGCCACTATCATCCTCCTTCTGCTTGCACAAGTTTCCTGGGCTGGACCGTTTCAACAGAGAGGCT
  TATTTGACTTTATGCTAGAAGATGAGGCTTCTGGGATAGGCCCAGAAGTTCCTGATGACCGCGACTTCGA
  GCCCTCCCTAGGCCCAGTGTGCCCCTTCCGCTGTCAATGCCATCTTCGAGTGGTCCAGTGTTCTGATTTG
  GGTCTGGACAAAGTGCCAAAGGATCTTCCCCCTGACACAACTCTGCTAGACCTGCAAAACAACAAAATAA
  CCGAAATCAAAGATGGAGACTTTAAGAACCTGAAGAACCTTCACGTTGTCTACCTTCATAACAACAATAT
  CTCTGTAGTTGGATCAAGTGACTTCTGCCCACCTGGACACAACACCAAAAAGGCTTCTTATTCGGGTGTG
  AGTCTTTTCAGCAACCCGGTCCAGTACTGGGAGATACAGCCATCCACCTTCAGATGTGTCTACGTGCGCT
  CTGCCATTCAACTCGGAAACTATAAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133506 unedited
  TGGATTTAGGTATCACGACTCACTATAGGGNCGGACCGCGCAATTCGGCACGAGTTTCAA
  CCTATTCACAGAGCAGCACCTACCCCCTCCTCCTTTCCCACCTGCAAACTCTTTTACTTG
  GGCTGAATATTTAGTGTAATTACATCTCAGCTTTGAGGGCTCCTGTGGCAAATTCCCGGA
  TTAAAAGGTTCCCTGGTTGTGAAAATACATGAGTTATTTTTATGAAGGCCACTATCATCC
  TCCTTCTGCTTGCACAAGTTTCCTGGGCTGGACCGTTTCAACAGAGAGGCTTATTTGACT
  TTATGCTAGAAGATGAGGCTTCTGGGATAGGCCCAGAAGTTCCTGATGACCGCGACTTCG
  AGCCCTCCCTAGGCCCAGTGTGCCCCTTCCGCTGTCAATGCCATCTTCGAGTGGTCCAGT
  GTTCTGATTTGGGTCTGGACAAAGTGCCAAAGGATCTTCCCCCTGACACAACTCTGCTAG
  ACCTGCAAAACAACAAAATAACCGAAATCAAAGATGGAGACTTTAAGAACCTGAAGAACC
  TTCACGCATTGATTCTTGTCAACAATAAAATTAGCAAAGTTAGTCCTGGAGCATTTACAC
  CTTTGGTGAAGTTGGAACGACTTTATCTGTCCAAGAATCAGCTGAAGGAATTGCCAGAAA
  AAATGCCCAAAACTCTTCAGGAGCTGCGTGCCCATGAGAATGAGATCACCAAAGTGCGAA
  AAGTACTTTTCATGGACTGAACCAGATGATTGTCATAGAACTTGGCACCAATCCGCTGAA
  GAGCTCAGGAATTGAAAATGGGGCTTTCCAGGGAATGAAGAAGCTCTCCTACATTCGCAT
  TGCTGATCCCATATCACCAGCATTCTCAGGTCTTACTCCTACCTTACCGAATACTCTTGA
  TGGCACCAAATCACCAAATTGATGCACCTGCCTGAAGGACTGAAAATATTGCTAAGTGGG
  ATTGGTTTAACGCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_133506
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133506.2, NP_598013.1
• RefSeq Size: 1336 bp
• RefSeq ORF: 519 bp
• Locus ID: 1634
• Cytogenetics: 12q21.33
• Domains: LRRNT, LRR
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: 'This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (D) differs in the 5' UTR and lacks four alternate exons in the coding region compared to variant A1. The encoded isoform (d) is shorter than isoform a. This isoform (d) may undergo proteolytic processing similar to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.