UBE3A (NM_130839) Human Untagged Clone

CAT#: SC120518

UBE3A (untagged)-Human ubiquitin protein ligase E3A (UBE3A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE3A
• Synonyms: ANCR; AS; E6-AP; EPVE6AP; HPVE6A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120518 sequence for NM_130839 edited (data generated by NextGen Sequencing)
  ATGGCCACAGCTTGTAAAAGATCAGGAGAACCTCAGTCTGACGACATTGAAGCTAGCCGA
  ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGC
  TGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATG
  GATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGT
  GATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCC
  CCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAA
  GATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGA
  GAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTG
  GTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCA
  AAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAANNTGCATGTTCTGCTGCTGCTATG
  GAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAAT
  TTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCCATTAGAAGGGTCTAC
  ACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTG
  TCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTAATTAT
  CTGAATTTGTTCATTATCGTAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAA
  ATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAA
  CTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTT
  CAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAAT
  GATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAAATGTA
  GTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAAGAGCCCATCCCTGAG
  TCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAAGAAAGGTCCT
  CGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTT
  ATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGAT
  TATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATA
  TTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGAATTCGCATGTACAGT
  GAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAATCCATATTTG
  AGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTCCGGCTAGAGATGATC
  GCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAA
  GGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTC
  AATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCA
  TCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATT
  TACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGG
  AAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAA
  GATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGATATCA
  CAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGGTGATAAAATT
  CCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAAT
  AAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAA
  TCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGG
  AATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGAC
  TCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAACAGAAAAGA
  CTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGAGGACTAGGAAAATTA
  AAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGC
  TTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTG
  AAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA
  
  Clone variation with respect to NM_130839.2
  577 g=>n;578 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130839 unedited
  CCGTCACAATTGTAACCGACTCATATAGGCGGCCGCCCAATCCCAGCTCCTCAGAGTTTG
  GCGACATATGAGTTATTAAGCCTACGCTCAGATCAAGGTAGCAGCTAGACTGGTGTGACA
  ACCTGTTTTTAATCAGTGACTCAAAGCTGTGATCACCCTGATGTCACCGAATGGCCACAG
  CTTGTAAAAGATCAGGAGAACCTCAGTCTGACGACATTGAAGCTAGCCGAATGAAGCGAG
  CAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATG
  AAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATG
  CAGCAGCTATTAAAGCCCTCGAGCTTTATAAGAGTAATGCAAAACTCTGTGATCCTCATC
  CCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACT
  CCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAAGATGTGACTT
  ACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATT
  CCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCT
  TCCGCAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAG
  ACAAAGATGAAGATGACCAGGACAAAGCTGCATGTTCTGCTGCTGCTATGGAAAGAGACT
  CAGAAGCATCTTTCTCAGGATAGGTGATAGCTCACAGGGAGACAGCAATTTGCAAAATTA
  GGCCCTTGATGATGTGTCTGTGGAATATTGATGCCATTCAAAGGGTCTTCCCCCAGATTG
  CTCTCCTAAGCAACAAAATGAACTGTCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130839 unedited
  CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTCCTTTTTTTTGTTTTA
  TTTTGTTTTGTTTTGTTTTACAGCATGCCAAATCCTTTGGCATACGTGATGGCCTTCAAC
  AATCTCTCTTTAAGTTTTTCTTTGCTTGAGTATTCCGGAAGTAAAAGCACATTAAAGCAA
  GTATGAGATGTAGGTAACCTTTCTGTGTCTGGGCCATTTTTGGCTATAATCATCTTTAAT
  TTTCCTAGTCCTCCCACAGGTGCTCTGTCTGTGCCCGTTGTAAACTGCAAGAAGAGTCTT
  TTCTGTTCATCTGTAAATGAATGAACGATTTCCCAGAACTCCCTAATCAGAACAGAGTCC
  CTGGTATAGCCACCGTCATATTCTGTAGTTTCTTCTAGTGCTTGGAAATCTAGATTCCGG
  CTTCCACATATAAGCAATTCAATTTCTTCTGGTCTGAATAAGTACTTTAAGGGAGATTCA
  TTGGTCACCATATGAAAACCTCTCCGAAAAGCCTTGAACTGTTTTTCTACTGATTTATTG
  AGAATGTAGTCAGAATAAAGATTGACAAATTCCTTCCTGTTTTCATTTGTAATTGGAATT
  TTATCACCATTTTCCTTTAGATCATACATCATTGGGTTACCAAAAAGATCTGTCTGTGAT
  ATCTGGAAAGTGATCATCATGTCATCTTCCACATTCCCTTCATACTCCAATAAATCTTTT
  AAACTCTGATATAGAACTGGGTGAGAGTCTCCCAGTCACGAAAAGTTCCTTTTTCCCCAT
  TAGCTNNCTGTAGACACCATGGGAAAATGTCATCCAGATACAGTTATTGTAAATAGCAGA
  CCAGTACTATGCCATCAGAGTAACTGACCCTCAGTTNCAAAGAGATGGATAANCCANNAC
  ATTTGTAGATCATCGTTGTGAACTACCATATCTGGNATGAGATTCCTCCCACCGCTGAAA
  AATCTTGGAACACTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_130839
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130839.1, NP_570854.1
• RefSeq Size: 5164 bp
• RefSeq ORF: 2619 bp
• Locus ID: 7337
• Cytogenetics: 15q11.2
• Domains: HECT
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR, and uses an alternate start codon compared to variant 2. The encoded isoform (3) has a distinct N-terminus and is 3 and is shorter than isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.