CMBL (NM_138809) Human Untagged Clone

CAT#: SC120612

CMBL (untagged)-Human carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CMBL
• Synonyms: JS-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120612 sequence for NM_138809 edited (data generated by NextGen Sequencing)
  ATGGCTAACGAAGCTTATCCTTGTCCGTGTGACATTGGCCACAGACTTGAGTATGGAGGG
  CTAGGCCGTGAAGTTCAAGTCGAGCACATCAAGGCTTATGTCACCAAATCCCCCGTTGAT
  GCAGGCAAAGCTGTGATTGTCATTCAAGATATATTTGGCTGGCAGTTGCCCAATACCAGA
  TATATAGCTGACATGATCTCAGGAAATGGATACACAACCATTGTTCCAGACTTCTTTGTA
  GGGCAAGAGCCTTGGGACCCCTCTGGCGACTGGTCTATCTTCCCTGAGTGGCTGAAAACA
  AGAAATGCCCAGAAGATCGATAGAGAGATCAGTGCTATCTTGAAGTATCTGAAACAACAG
  TGTCATGCCCAGAAAATTGGCATCGTGGGATTCTGCTGGGGTGGAACTGCTGTCCATCAT
  TTGATGATGAAATACTCAGAATTCAGGGCAGGGGTGTCCGTCTATGGCATCGTCAAGGAT
  TCTGAAGACATTTACAATTTAAAGAACCCCACTTTGTTCATTTTTGCTGAAAATGATGTT
  GTGATTCCACTCAAGGACGTATCTTTGCTGACTCAGAAGTTGAAAGAACACTGCAAAGTT
  GAATATCAAATTAAAACATTTTCTGGGCAGACTCATGGGTTCGTGCATCGGAAGAGAGAA
  GATTGCTCACCTGCAGACAAGCCCTACATTGACGAGGCCAGAAGGAATTTAATTGAGTGG
  CTGAACAAGTACATGTAG
  
  Clone variation with respect to NM_138809.3
  471 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138809 unedited
  GCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCCGCGTCCA
  CGTCCCTCTCGCGTCCCCGCCCGGCGCCACGCCGCCTCCTCTGGGTTCGGCCTCCGCGCG
  GTGCAGCGCAGTCTCAGGCCGCGGGACAAGCCCGACTTAAATCTCTGCAATGGCTAACGA
  AGCTTATCCTTGTCCGTGTGACATTGGCCACAGACTTGAGTATGGAGGGCTAGGCCGTGA
  AGTTCAAGTCGAGCACATCAAGGCTTATGTCACCAAATCCCCCGTTGATGCAGGCAAAGC
  TGTGATTGTCATTCAAGATATATTTGGCTGGCAGTTGCCCAATACCAGATATATAGCTGA
  CATGATCTCAGGAAATGGATACACAACCATTGTTCCAGACTTCTTTGTAGGGCAAGAGCC
  TTGGGACCCCTCTGGCGACTGGTCTATCTTCCCTGAGTGGCTGAAAACAAGAAATGCCCA
  GAAGATCGATAGAGAGATCAGTGCTATCTTGAAGTATCTGAAACAACAGTGTCATGCCCA
  GAAAATTGGCATCGTGGGATTCTGCTGGGGTGGAACTGCTGTCCATCATTTGATGATGAA
  ATACTCAGAATTCAGGGCAGGNGGTGTCCGTCTATGGCATCGTCAAGGATTCTGAAGACA
  TTTACAATTTAAAGAACCCCACTTTGTTCATTTTTGCTGAAAATGATGTTGTGATTCCAC
  TCAAGGACGTATCTTTGCTGACTCAGAAGTTGAAAGAACACTNGCAAGTTGAATATCAAA
  TTAAAACATTTTCTGGGCAGACTCATGGGGTTCGTGCATCGGAAGAGAGAAGATTGCTCA
  CCTGCAGACAAGCCCTACATTGACGAGGCCAGAAGGAATTNAATTGAGTGGCTGAACAAG
  TACATGTAGCAAGAATCAGGGGCAGCCCTTCTAGNATAGCTTTCATNCCAAAATTGCTGG
  NAATAGNTAGATCATTTNGATTAATTTAACCTTATAAAAATAGTGTAGGATN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138809 unedited
  NGGTTTTATCTATGNACGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTGGTTTTTTTTTTTTTTTGGGGGTTTTTTTAAAAATCAAAAAATAGTTTA
  TTAGCTTAATATAATTAGGGCAATGGGAATCCCGTTTTGATCCCAATACTTCCCATATTG
  GAATATATAAATGGGACAAATTCCCCTGTTTTGGGGCATAAATAAATGGCTAAACCGGGC
  AATTAGGCGACCCGGTTATAGTTCAAGGTCTTCAAGGGTTTGCAACATTTGGCACTTTTT
  ATGAAGGGGTCAAAAACCCAAAAAAGGCCCACTTGACCTGGCAACTGGGCCTTGAACAAC
  TGGAGGTTTTTTTTTTTTGAAAAAAAATCTCCCTCTGTTGTCCAAGCTCGGAGTGCAATA
  ACCCAACCCCGACTCACTGGAACCCCTGGCCCCAAGTTCAAAGGATTCTCCCCCCCAAAC
  CCCCGAATAGCTGGGATTACAGGGCCATGCCCCCCTGCCCGGGGGATTTTTATATTTTTA
  AGAAAAAAGGGGTTTCACCATGTTGGCCAAGCCGGGCTTGAACCCCTGACCCCAAGTGGT
  CCACCCACCTCGGCCTCCCAAAATGCTGGGATTACAGGGGTGAGCCACCACACCCGGCCC
  GGAGGGTTTAACCAATAATTAGGGGATTACCCTATCATTCCCTATCATTCAAATTCTCCA
  AGGACCGACCCGGGTTTAAATTTAATTAATTAATTATTTAACCTAATTAATTTTGAAACA
  AGGGCTTAACTCGGTTGCCCAAGCTAAATACAATAAGGGGGCCAATCCTGGGGTCCCTGG
  AGTCTCCACCCCCGGGGTCCTATTGAATTCCTGGGCCCTCACCCCCCCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138809
• ORF Size: 738 bp
• Insert Size: 2250
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_138809.2, NP_620164.1
• RefSeq Size: 2156
• RefSeq ORF: 738
• Locus ID: 134147
• Domains: DLH
• Protein Pathways: Metabolic pathways
• Gene Summary: CMBL (EC 3.1.1.45) is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom (Ishizuka et al., 2010 [PubMed 20177059]). [supplied by OMIM, Apr 2010]