TSSC3 (PHLDA2) (NM_003311) Human Untagged Clone

CAT#: SC120954

PHLDA2 (untagged)-Human pleckstrin homology-like domain, family A, member 2 (PHLDA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHLDA2
• Synonyms: BRW1C; BWR1C; HLDA2; IPL; TSSC3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003311 edited
  CCGTCACCGCCCGCATTGCCGCTCCCAGTCCCGCGCTCGGCACGACATGAAATCCCCCGA
  CGAGGTGCTACGCGAGGGCGAGTTGGAGAAGCGCAGCGACAGCCTCTTCCAGCTATGGAA
  GAAGAAGCGCGGGGTGCTCACCTCCGACCGCCTGAGCCTGTTCCCCGCCAGCCCCCGCGC
  GCGCCCCAAGGAGCTGCGCTTCCACTCCATCCTCAAGGTGGACTGCGTGGAGCGCACGGG
  CAAGTACGTGTACTTCACCATCGTCACCACCGACCACAAGGAGATCGACTTCCGCTGCGC
  GGGCGAGAGCTGCTGGAACGCGGCCATCGCGCTGGCGCTCATCGATTTCCAGAACCGCCG
  CGCCCTGCAGGACTTTCGCAGCCGCCAGGAACGCACCGCACCCGCCGCACCCGCCGAGGA
  CGCCGTGGCTGCCGCGGCCGCCGCACCCTCCGAGCCCTCGGAGCCCTCCAGGCCATCCCC
  GCAGCCCAAACCCCGCACGCCATGAGCCCGCCGCGGGCCATACGCTGGACGAGTCGGACC
  GAGGCTAGGACGTGGCCGGCGCTCTCCAGCCCTGCAGCAGAAGAACTTCCCGTGCGCGCG
  GATCCTCGCTCCGTTGCACGGGCGCCTTAAGTTATTGGACTATCTAATATCTATGTATTT
  ATTTCGCTGGTTCTTTGTAGTCACATATTTTATAGTCTTAATATCTTGTTTTTGCATCAC
  TGTGCCCATTGCAAATAAATCACTTGGCCAGTTTGCTTTTCTACCAAAAAAAAAAAAAAA
  AAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003311 unedited
  AAAACCCTTCGGGATTTGTNATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGG
  CCGTCACCGCCCGCATTGCCGCTCCCAGTCCCGCGCTCGGCACGACATGAAATCCCCCGA
  CGAGGTGCTACGCGAGGGCGAGTTGGAGAAGCGCAGCGACAGCCTCTTCCAGCTATGGAA
  GAAGAAGCGCGGGGTGCTCACCTCCGACCGCCTGAGCCTGTTCCCCGCCAGCCCCCGCGC
  GCGCCCCAAGGAGCTGCGCTTCCACTCCATCCTCAAGGTGGACTGCGTGGAGCGCACGGG
  CAAGTACGTGTACTTCACCATCGTCACCACCGACCACAAGGAGATCGACTTCCGCTGCGC
  GGGCGAGAGCTGCTGGAACGCGGCCATCGCGCTGGCGCTCATCGATTTCCAGAACCGCCG
  CGCCCTGCAGGACTTTCGCAGCCGCCAGGAACGCACCGCACCCGCCGCACCCGCCGAGGA
  CGCCGTGGCTGCCGCGGCCGCCGCACCCTCCGAGCCCTCGGAGCCCTCCAGGCCATCCCC
  GCAGCCCAAACCCCGCACGCCATGAGCCCGCCGCGGGCCATACGCTGGACGAGTCGGACC
  GAGGCTAGGACGTGGCCGGCGCTCTCCAGCCCTGCAGCAGAAGAACTTCCCGTGCGCGCG
  GATCCTCGCTCCGTTGCACGGGCGCCTTAAGTTATTGGACTATCTAATATCTATGTATTT
  ATTTCGCTGGTTCTTTGTAGTCACATATTTTATAGTCTTAATATCTTGTTTTTGCATCAC
  TGTGCCCATTGCAAATAAATCACTTGGCCAGTTTGCTTNTCTACCAAAAAAAAAANAAAA
  AAACTCGACTCA
  
• Restriction Sites: Please inquire
• ACCN: NM_003311
• Insert Size: 784 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003311.3, NP_003302.1
• RefSeq Size: 937 bp
• RefSeq ORF: 459 bp
• Locus ID: 7262
• Cytogenetics: 11p15.4
• Protein Families: Druggable Genome
• Gene Summary: 'This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver. [provided by RefSeq, Oct 2010]'