RAB6A (NM_198896) Human Untagged Clone

CAT#: SC121231

RAB6A (untagged)-Human RAB6A, member RAS oncogene family (RAB6A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB6A
• Synonyms: RAB6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121231 sequence for NM_198896 edited (data generated by NextGen Sequencing)
  ATGTCCACGGGCGGAGACTTCGGGAATCCGCTGAGGAAATTCAAGCTGGTGTTCCTGGGG
  GAGCAAAGCGTTGGAAAGACATCTTTGATCACCAGATTCATGTATGACAGTTTTGACAAC
  ACCTATCAGGCAACAATTGGCATTGACTTTTTATCAAAAACTATGTACTTGGAGGATCGA
  ACAGTACGATTGCAATTATGGGACACAGCAGGTCAAGAGCGGTTCAGGAGCTTGATTCCT
  AGCTACATTCGTGACTCCACTGTGGCAGTTGTTGTTTATGATATCACAAATGTTAACTCA
  TTCCAGCAAACTACAAAGTGGATTGATGATGTCAGAACAGAAAGAGGAAGTGATGTTATC
  ATCATGCTAGTAGGAAATAAAACAGATCTTGCTGACAAGAGGCAAGTGTCAATTGAGGAG
  GGAGAGAGGAAAGCCAAAGAGCTGAATGTTATGTTTATTGAAACTAGTGCAAAAGCTGGA
  TACAATGTAAAGCAGCTCTTTCGACGTGTAGCAGCAGCTTTGCCGGGAATGGAAAGCACA
  CAGGACAGAAGCAGAGAAGATATGATTGACATAAAACTGGAAAAGCCTCAGGAGCAACCA
  GTCAGTGAAGGAGGCTGTTCCTGCTAA
  
  Clone variation with respect to NM_198896.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198896 unedited
  ACATTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGCCGCTCCTCT
  ATTCCCATGTCCACGGGCGGAGACTTCGGGAATCCGCTGAGGAAATTCAAGCTGGTGTTC
  CTGGGGGAGCAAAGCGTTGGAAAGACATCTTTGATCACCAGATTCATGTATGACAGTTTT
  GACAACACCTATCAGGCAACAATTGGCATTGACTTTTTATCAAAAACTATGTACTTGGAG
  GATCGAACAGTACGATTGCAATTATGGGACACAGCAGGTCAAGAGCGGTTCAGGAGCTTG
  ATTCCTAGCTACATTCGTGACTCCACTGTGGCAGTTGTTGTTTATGATATCACAAATGTT
  AACTCATTCCAGCAAACTACAAAGTGGATTGATGATGTCAGAACAGAAAGAGGAAGTGAT
  GTTATCATCATGCTAGTAGGAAATAAAACAGATCTTGCTGACAAGAGGCAAGTGTCAATT
  GAGGAGGGAGAGAGGAAAGCCAAAGAGCTGAATGTTATGTTTATTGAAACTAGTGCAAAA
  GCTGGATACAATGTAAAGCAGCTCTTTCGACGTGTAGCAGCAGCTTTGCCGGGAATGGAA
  AGCACACAGGACAGAAGCAGAGAAGATATGATTGACATAAAACTGGAAAAGCCTCAGGAG
  CAACCAGTCAGTGAAGGAGGCTGTTCCTGCTAATCTCCCATGTCATCTTCAACCTTCTTC
  AGAAGCTCACTGCTNNTGGCCCCTTACTCTNTCATTGACTGCAGTGTGAATATTGGCTTG
  AACCTTTCCCTTCGTAATAACGTATTGCAATTCATCATTGCTGCCTGTCTCGTGGAGATG
  ATCTATTAGCTTCACAAGCACACAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198896
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198896.1, NP_942599.1
• RefSeq Size: 3419 bp
• RefSeq ORF: 627 bp
• Locus ID: 5870
• Cytogenetics: 11q13.4
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) directions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (2) has an alternate exon with the same size in the coding region, compared to variant 1. The resulting isoform (b, also known as RAB6A) is the same size but has three different aa in an internal segment, compared to isoform a.