CD56 (NCAM1) (NM_181351) Human Untagged Clone

CAT#: SC121351

NCAM1 (untagged)-Human neural cell adhesion molecule 1 (NCAM1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCAM1
• Synonyms: CD56; MSK39; NCAM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121351 sequence for NM_181351 edited (data generated by NextGen Sequencing)
  ATGCTGCAAACTAAGGATCTCATCTGGACTTTGTTTTTCCTGGGAACTGCAGTTTCTCTG
  CAGGTGGATATTGTTCCCAGCCAGGGGGAGATCAGCGTTGGAGAGTCCAAATTCTTCTTA
  TGCCAAGTGGCAGGAGATGCCAAAGATAAAGACATCTCCTGGTTCTCCCCCAATGGAGAA
  AAGCTCACCCCAAACCAGCAGCGGATCTCAGTGGTGTGGAATGATGATTCCTCCTCCACC
  CTCACCATCTATAACGCCAACATCGACGACGCCGGCATTTACAAGTGTGTGGTTACAGGC
  GAGGATGGCAGTGAGTCAGAGGCCACCGTCAACGTGAAGATCTTTCAGAAGCTCATGTTC
  AAGAATGCGCCAACCCCACAGGAGTTCCGGGAGGGGGAAGATGCCGTGATTGTGTGTGAT
  GTGGTCAGCTCCCTCCCACCAACCATCATCTGGAAACACAAAGGCCGAGATGTCATCCTG
  AAAAAAGATGTCCGATTCATAGTCCTGTCCAACAACTACCTGCAGATCCGGGGCATCAAG
  AAAACAGATGAGGGCACTTATCGCTGTGAGGGCAGAATCCTGGCACGGGGGGAGATCAAC
  TTCAAGGACATTCAGGTCATTGTGAATGTGCCACCTACCATCCAGGCCAGGCAGAATATT
  GTGAATGCCACCGCCAACCTCGGCCAGTCCGTCACCCTGGTGTGCGATGCCGAAGGCTTC
  CCAGAGCCCACCATGAGCTGGACAAAGGATGGGGAACAGATAGAGCAAGAGGAAGACGAT
  GAGAAGTACATCTTCAGCGACGATAGTTCCCAGCTGACCATCAAAAAGGTGGATAAGAAC
  GACGAGGCTGAGTACATCTGCATTGCTGAGAACAAGGCTGGCGAGCAGGATGCGACCATC
  CACCTCAAAGTCTTTGCAAAACCCAAAATCACATATGTAGAGAACCAGACTGCCATGGAA
  TTAGAGGAGCAGGTCACTCTTACCTGTGAAGCCTCCGGAGACCCCATTCCCTCCATCACC
  TGGAGGACTTCTACCCGGAACATCAGCAGCGAAGAAAAGGCTTCGTGGACTCGACCAGAG
  AAGCAAGAGACTCTGGATGGGCACATGGTGGTGCGTAGCCATGCCCGTGTGTCGTCGCTG
  ACCCTGAAGAGCATCCAGTACACTGATGCCGGAGAGTACATCTGCACCGCCAGCAACACC
  ATCGGCCAGGACTCCCAGTCCATGTACCTTGAAGTGCAATATGCCCCAAAGCTACAGGGC
  CCTGTGGCTGTGTACACTTGGGAGGGGAACCAGGTGAACATCACCTGCGAGGTATTTGCC
  TATCCCAGTGCCACGATCTCATGGTTTCGGGATGGCCAGCTGCTGCCAAGCTCCAATTAC
  AGCAATATCAAGATCTACAACACCCCCTCTGCCAGCTATCTGGAGGTGACCCCAGACTCT
  GAGAATGATTTTGGGAACTACAACTGTACTGCAGTGAACCGCATTGGGCAGGAGTCCTTG
  GAATTCATCCTTGTTCAAGCAGACACCCCCTCTTCACCATCCATCGACCAGGTGGAGCCA
  TACTCCAGCACAGCCCAGGTGCAGTTTGATGAACCAGAGGCCACAGGTGGGGTGCCCATC
  CTCAAATACAAAGCTGAGTGGAGAGCAGTGGGTGAAGAAGTATGGCATTCCAAGTGGTAT
  GATGCCAAGGAAGCCAGCATGGAGGGCATCGTCACCATCGTGGGCCTGAAGCCCGAAACA
  ACGTACGCCGTAAGGCTGGCGGCGCTCAATGGCAAAGGGCTGGGTGAGATCAGCGCGGCC
  TCCGAGTTCAAGACGCAGCCAGTCCNNNGGGAACCCAGTGCACCTAAGCTCGAAGGGCAG
  ATGGGAGAGGATGGAAACTCTATTAAAGTGAACCTGATCAAGCAGGATGACGGCGGCTCC
  CCCATCAGACACTATCTGGTCAGGTACCGAGCGCTCTCCTCCGAGTGGAAACCAGAGATC
  AGGCTCCCGTCTGGCAGTGACCACGTCATGCTGAAGTCCCTGGACTGGAATGCTGAGTAT
  GAGGTCTACGTGGTGGCTGAGAACCAGCAAGGAAAATCCAAGGCGGCTCATTTTGTGTTC
  AGGACCTCGGCCCAGCCCACAGCCATCCCAGCCAACGGCAGCCCCACCTCAGGCCTGAGC
  ACCGGGGCCATCGTGGGCATCCTCATCGTCATCTTCGTCCTGCTCCTGGTGGTTGTGGAC
  ATCACCTGCTACTTCCTGAACAAGTGTGGCCTGTTCATGTGCATTGCGGTCAACCTGTGT
  GGAAAAGCCGGGCCCGGGGCCAAGGGCAAGGACATGGAGGAGGGCAAGGCCGCCTTCTCG
  AAAGATGAGTCCAAGGAGCCCATCGTGGAGGTTCGAACGGAGGAGGAGAGGACCCCAAAC
  CATGATGGAGGGAAACACACAGAGCCCAACGAGACCACGCCACTGACGGAGCCCGAGAAG
  GGCCCCGTAGAAGCAAAGCCAGAGTGCCAGGAGACAGAAACGAAGCCAGCGCCAGCCGAA
  GTCAAGACGGTCCCCAATGACGCCACACAGACAAAGGAGAACGAGAGCAAAGCATGA
  
  Clone variation with respect to NM_181351.4
  1650 t=>g;1826 a=>n;1827 a=>n;1828 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181351 unedited
  GAGGGTGAACGCAGCTCGGCTGCCGCTGGCAGGNAAACAATTCTGCAAAAATAATCATAC
  TCAGCCTGGCAATTGTCTGCCCCTAGGTCTGTCGCTCAGCCGCCGTCCACACTCGCTGCA
  GGGGGGGGGGCACAGAATTTACCGCGGCAAGAACATCCCTCCCAGCCAGCAGATTACAAT
  GCTGCAAACTAAGGATCTCATCTGGACTTTGTTTTTCCTGGGAACTGCAGTTTCTCTGCA
  GGTGGATATTGTTCCCAGCCAGGGGGAGATCAGCGTTGGAGAGTCCAAATTCTTCTTATG
  CCAAGTGGCAGGAGATGCCAAAGATAAAGACATCTCCTGGTTCTCCCCCAATGGAGAAAA
  GCTCACCCCAAACCAGCAGCGGATCTCAGTGGTGTGGAATGATGATTCCTCCTCCACCCT
  CACCATCTATAACGCCAACATCGACGACGCCGGCATTTACAAGTGTGTGGTTACAGGCGA
  GGATGGCAGTGAGTCAGAGGCCACCGTCAACGTGAAGATCTTTCAGAAGCTCATGTTCAA
  GAATGCGCCAACCCCACAGGAGTTCCGGGAGGGGGAAGATGCCGTGATTGTGTGTGATGT
  GGTCAGCTNCCTCCCACCAACCATCATCTGGAAACACAAAGGCCGAGATGTCATCCTGAA
  AAAAGATGTCCGATTCATAGTCCTGTCCAACAACTACCTGCAGATCCGGGGCATCAAGAA
  AACAGATGAGGGGCACTTATCGCTGTGAGGGCANAATCCTGGCNCGGGGGGGAGAACAAC
  TTCAAGGACATTTCAGGTCATTGTGAATGTGCCACCTACCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181351
• Insert Size: 5400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181351.1, NP_851996.1
• RefSeq Size: 4050 bp
• RefSeq ORF: 2577 bp
• Locus ID: 4684
• Cytogenetics: 11q23.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Prion diseases
• Gene Summary: 'This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein plays a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. This protein is also involved in the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. This protein plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this gene, cell surface molecule CD56, plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of this gene is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 5. The resulting protein (isoform 2) is shorter compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.