TCF7 (NM_201633) Human Untagged Clone

CAT#: SC121841

TCF7 (untagged)-Human transcription factor 7 (T-cell specific, HMG-box) (TCF7), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF7
• Synonyms: FLJ36364; MGC47735; OTTHUMP00000159391; TCF-1; Transcription factor-7, T-cell specific; transcription factor 7 (T-cell specific, HMG-box)
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_201633, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTACAAAGAGACCGTCTACTCCGCCTTCAATCTGCTCATGCATTACCCACCCCCCTCGGGAGCAGGGC
  AGCACCCCCAGCCGCAGCCCCCGCTGCACAAGGCCAATCAGCCCCCCCACGGTGTCCCCCAACTCTCTCT
  CTACGAACATTTCAACAGCCCACATCCCACCCCTGCACCTGCGGACATCAGCCAGAAGCAAGTTCACAGG
  CCTCTGCAGACCCCTGACCTCTCTGGCTTCTACTCCCTGACCTCAGGCAGCATGGGGCAGCTCCCCCACA
  CTGTGAGCTGGTTCACCCACCCATCCTTGATGCTAGGTTCTGGTGTACCTGGTCACCCAGCAGCCATCCC
  CCACCCGGCCATTGTGCCCCCCTCAGGGAAGCAGGAGCTGCAGCCCTTCGACCGCAACCTGAAGACACAA
  GCAGAGTCCAAGGCAGAGAAGGAGGCCAAGAAGCCAACCATCAAGAAGCCCCTCAATGCCTTCATGCTGT
  ACATGAAGGAGATGAGAGCCAAGGTCATTGCAGAGTGCACACTTAAGGAGAGCGCTGCCATCAACCAGAT
  CCTGGGCCGCAGGTGGCACGCGCTGTCGCGAGAAGAGCAGGCCAAGTACTATGAGCTGGCCCGCAAGGAG
  AGGCAGCTGCACATGCAGCTATACCCAGGCTGGTCAGCGCGGGACAACTACGGGAAGAAGAAGAGGCGGT
  CGAGGGAAAAGCACCAAGAATCCACCACAGAGACAAACTGGCCCAGAGAACTCAAGGATGGTAATGGACA
  AGAGTCACTGTCCATGTCTTCTTCCTCTAGCCCAGCTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_201633 unedited
  GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGGGCCGGCGCCGGGGCCCGCG
  GCGAGGCCGAGGCTCTCGGGCGGGAACACGCTGCGCAGAGACTCTTCCCGGACAAACTTC
  CAGAGCCCCTGGAGGACGGCCTGAAGGCCCCGGAGTGCACCAGCGGCATGTACAAAGAGA
  CCGTCTACTCCGCCTTCAATCTGCTCATGCATTACCCACCCCCCTCGGGAGCAGGGCAGC
  ACCCCCAGCCGCAGCCCCCGCTGCACAAGGCCAATCAGCCCCCCCACGGTGTCCCCCAAC
  TCTCTCTCTACGAACATTTCAACAGCCCACATCCCACCCCTGCACCTGCGGACATCAGCC
  AGAAGCAAGTTCACAGGCCTCTGCAGACCCCTGACCTCTCTGGCTTCTACTCCCTGACCT
  CAGGCAGCATGGGGCAGCTCCCCCACACTGTGAGCTGGCCCAGCCCTCCTCTCTACCCCC
  TGTCCCCTTCCTGCGGATATAGACANGCACTTCCCTGCCCCCACTGCAGCCCCTGGCGCC
  CCCTACCCCAGGTTCACCCACCCATCCTTGATGCTAGGTTCTGGTGTACCTGGTCACCCA
  GCAGCCATCCCCCACCCGGCCATTGTGCCCCCCTCAGGGAAAGCAGAGCTGCAGCCCTTC
  GACNCCGCACCTGAGACACAAGCAGAGTCCAAGGCAGAGAAGGAGGCCAAGAAGCCAACC
  ATCAAGAAGCCCCTCATGCCTTCATGCTGTACATGAAGGGAGATGAGAGCCCAGGTCATT
  GCAGAGTGACACTTAAAGAGAGCGCTGCATCACCAGATCCTGGCCGCGAGTGCACGCGCT
  GTCGCAGAAAAAGCAGCCCAGTACTATGACCTGCCCCGAAGGAAAGGCAGCTGCCATGCA
  CTATCCCAGCTGGTCACGCGGAACTACGGAAAAAAAAAAAGCGGGCCGGGGAAACCCCAG
  AATCCCACGGAGAAAGATTGCCTCGACTACCCCGGAAAGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_201633
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201633.1, NP_963964.1
• RefSeq Size: 1254 bp
• RefSeq ORF: 810 bp
• Locus ID: 6932
• Cytogenetics: 5q31.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Melanogenesis, Pathways in cancer, Prostate cancer, Thyroid cancer, Wnt signaling pathway
• Gene Summary: 'This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling. [provided by RefSeq, Oct 2016]'
  Transcript Variant: This variant (3) differs in the 5' UTR and has multiple differences in the 5' and 3' coding region, compared to variant 1. These differences cause translation initiation from a downstream ATG and a protein isoform (3) with a distinct C-terminus, compared to isoform 1.