PPP2R1A (BC001537) Human Untagged Clone

CAT#: SC121908

PPP2R1A (untagged)-Homo sapiens, clone MGC:786 IMAGE:2987938, complete cds


Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP2R1A
Synonyms alpha isoform of regulatory subunit A, protein phosphatase 2; medium tumor antigen-associated 61 KDA protein; MGC786; PP2A, subunit A, PR65-alpha isoform; PP2A, subunit A, R1-alpha isoform; PR65A; protein phosphatase 2 (formerly 2A), regulatory subunit A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for BC001537, the custom clone sequence may differ by one or more nucleotides


ATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGACGAACTCCGCAATGAGG
ACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATCGCCTTGGCCCTTGGGGTTGAAAGGAC
CCGAAGTGAGCTTCTGCCTTTCCTTACAGATACCATCTATGATGAAGATGAGGTCCTCCTGGCCCTGGCA
GAACAGCTGGGAACCTTCACTACCCTGGTGGGAGGCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGG
AGTCGCTGGCCACAGTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACA
CGAGCACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGCGACTGG
TTCACCTCCCGCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTGTCCAGTGCTGTGAAGG
CGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATGGTGCGGCGGGCCGCAGCCTC
CAAGCTGGGGGAGTTTGCCAAGGTGCTGGAGCTGGACAACGTCAAGAGTGAGATCATCCCCATGTTCTCC
AACCTGGCCTCTGACGAGCAGGACTCGGTGCGGCTGCTGGCGGTGGAGGCGTGCGTGAACATCGCCCAGC
TTCTGCCCCAGGAGGATCTGGAGGCCCTGGTGATGCCCACTCTGCGCCAGGCCGCTGAAGACAAGTCCTG
GCGCGTCCGCTACATGGTGGCTGACAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAG
ACAGACCTGGTCCCTGCCTTCCAGAACCTGATGAAAGACTGTGAGGCCGAGGTGAGGGCCGCAGCCTCCC
ACAAGGTCAAAGAGTTCTGTGAAAACCTCTCAGCTGACTGTCGGGAGAATGTGATCATGTCCCAGATCTT
GCCCTGCATCAAGGAGCTGGTGTCCGATGCCAACCAACATGTCAAGTCTGCCCTGGCCTCAGTCATCATG
GGTCTCTCTCCCATCTTGGGCAAAGACAACACCATCGAGCACCTCTTGCCCCTCTTCCTGGCTCAGCTGA
AGGATGAGTGCCCTGAGGTACGGCTGAACATCATCTCTAACCTGGACTGTGTGAACGAGGTGATTGGCAT
CCGGCAGCTGTCCCAGTCCCTGCTCCCTGCCATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGG
CTGGCCATCATTGAGTACATGCCCCTCCTGGCTGGACAGCTGGGAGTGGAGTTCTTTGATGAGAAACTTA
ACTCCTTGTGCATGGCCTGGCTTGTGGATCATGTATATGCCATCCGCGAGGCAGCCACCAGCAACCTGAA
GAAGCTAGTGGAAAAGTTTGGGAAGGAGTGGGCCCATGCCACAATCATCCCCAAGGTCTTGGCCATGTCC
GGAGACCCCAACTACCTGCACCGCATGACTACGCTCTTCTGCATCAATGTGCTGTCTGAGGTCTGTGGGC
AGGACATCACCACCAAGCACATGCTACCCACGGTTCTGCGCATGGCTGGGGACCCGGTTGCCAATGTCCG
CTTCAATGTGGCCAAGTCTCTGCAGAAGATAGGGCCCATCCTGGACAACAGCACCTTGCAGAGTGAAGTC
AAGCCCATCCTAGAGAAGCTGACCCAGGACCAGGATGTGGACGTCAAATACTTTGCCCAGGAGGCTCTGA
CTGTTCTGTCTCTCGCCTGA


>OriGene 5' read for BC001537 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCTGACAGGAAAGGACGGA
GCCAAGATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGAC
GAACTCCGCAATGAGGACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATC
GCCTTGGCCCTTGGGGTTGAAAGGACCCGAAGTGAGCTTCTGCCTTTCCTTACAGATACC
ATCTATGATGAAGATGAGGTCCTCCTGGCCCTGGCAGAACAGCTGGGAACCTTCACTACC
CTGGTGGGAGGCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGGAGTCGCTGGCCACA
GTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACACGAG
CACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGC
GACTGGTTCACCTCCCGCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTG
TCCAGTGCTGTGAAGGCGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACC
CCCATGGTGCGGCGGGCCGCAGCCTNCAAGCTGGNGGAGTTTGCCAAGGTGCTGGAGCTG
GNACACGTCAAGAGTGAGATCATCCCCCATGTCTCCAACCTGGCCTCTGACGAGCANGAC
NNTCGTGCGCTGCTGGCNGNGGNNAGCTTGCCTAAACATCNNCCAGCTTCTGCCCCAGGA
GATCTGGAGGCCCTGNGGATGCCCACTCTGCGCCACGCCGCTGAAAACAGTCCTGGCGCG
TCCGCTACATGTTGCTGACAAGTCACAGAGCTCAAAAGCAGGGGGGCTGAAATCACCAGA
AGAACTGGTCCCTGCTCCAAACCTGATGAAGACTGGAGGCGAGGTGANGCCC
>OriGene 3' read for BC001537 unedited
TTTCTCTGTGAAGGGGGAAATAAAGGATAAATAAAACGGCACAGTTGACACACAAAAAAA
AACCAATGATGGGGAGGACGGGAGGTGGAGAAGTAAATGGGGGAGGGGTTCCCATTACAG
CAGCAGGATCCAGTGACCCGGGATGCTCACATCTCTCCCTGACGTGGGCGGAGTAGCCCC
TTCCTCCCAAGGTCACTGTCCTGTCCAACCCCGTGCTCCCCTAGCCCGTTGGGAGGTGGA
CAGTGAGACATCTTCCCAGGCTGGGGAGAGGAGGAACCGTGCTGGGGGAAGGGGCCTGGG
GTCAGACCATGCACAGGGAGTGACAGCCAAAGGCCCCCCAGTGTCTCCCCAAAGAGGGAC
ACGTGGGGGTTGGAGGGTGGACACCAGAGGCCAGCGTTTGCTCCTCTTCCAGCATCAGGC
GAGAGACAGAACAGTCAGAGCCTCCTGGGCAAAGTATTTGACGTCCACATCCTGGTCCTG
GGTCAGCTTCTCTAGGATGGGCTCGACTTCACTCTGCCAGGTGCTGTTGCCCAGGATGGG
CCCTATCCTCTGAACAGACTTGGCCACATTGAAGCCGGACATTGGCACCCGGTCCCCCAC
CTTGCCCATTACCGTGGGCACCCTGTGCTTGTTGGTGAGGTCTTGCTACAGACCTATTAC
GTACAATTGCGCCTAAAAACTTTTCCCTGCCGCTCCGGTTAACTGGGCCCTCCCGCCTTG
GCCCACACCTTTGGGAAAATTTGCCCTGGGCCCCCTCCTTTCCCAACTTTTCCACTACCC
TTTTCAGGTTTCTCGGTGCTGTCTCCGCGTGGGTTTTACTCATTCCCAACCTCGCCACTC
ACTCGATTACCCCCCCACACCAACTTCCCCCCCCCCTTCTCCCGGAGTTATTTACCCACA
CGGTCACCCCCCTCTCCTGGCGTCCCCCCCACCCTTCATCCCTGAACCCCCCTGGACACT
TCTCCGCCCTCCTTTCTCCCATTCTCTCATCCTCCTCCTCCCCAGG
Restriction Sites NotI-NotI     
ACCN BC001537
Insert Size 2390 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC001537.2, AAH01537.1
RefSeq Size 2282 bp
Locus ID 5518
Cytogenetics 19q13.41
Protein Families Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
Gene Summary 'This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'

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