TRPC1 (NM_003304) Human Untagged Clone

CAT#: SC121934

TRPC1 (untagged)-Human transient receptor potential cation channel, subfamily C, member 1 (TRPC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPC1
• Synonyms: HTRP-1; TRP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003304 edited
  GGGGCCGGTGGGGGCCCCGCCCCCGTCTCCTGGCCTGCCCCCTTCATGGGCCGCGATGAT
  GGCGGCCCTGTACCCGAGCACGGACCTCTCGGGCGCCTCCTCCTCCTCCCTGCCTTCCTC
  TCCATCCTCTTCCTCGCCGAACGAGGTGATGGCGCTGAAGGATGTGCGGGAGGTGAAGGA
  GGAGAATACGCTGAATGAGAAGCTTTTCTTGCTGGCGTGCGACAAGGGTGACTATTATAT
  GGTTAAAAAGATTTTGGAGGAAAACAGTTCAGGTGACTTGAACATAAATTGCGTAGATGT
  GCTTGGGAGAAATGCTGTTACCATAACTATTGAAAACGAAAACTTGGATATACTGCAGCT
  TCTTTTGGACTACGGTTGTCAGAAACTAATGGAACGAATTCAGAATCCTGAGTATTCAAC
  AACTATGGATGTTGCACCTGTCATTTTAGCTGCTCATCGTAACAACTATGAAATTCTTAC
  AATGCTCTTAAAACAGGATGTATCTCTACCCAAGCCCCATGCAGTTGGCTGTGAATGCAC
  ATTGTGTTCTGCAAAAAACAAAAAGGATAGCCTCCGGCATTCCAGGTTTCGTCTTGATAT
  ATATCGATGTTTGGCCAGTCCAGCTCTAATAATGTTAACAGAGGAGGATCCAATTCTGAG
  AGCATTTGAACTTAGTGCTGATTTAAAAGAACTAAGTCTTGTGGAGGTGGAATTCAGGAA
  TGATTATGAGGAACTAGCCCGGCAATGTAAAATGTTTGCTAAGGATTTACTTGCACAAGC
  CCGGAATTCTCGTGAATTGGAAGTTATTCTAAACCATACGTCTAGTGACGAGCCTCTTGA
  CAAACGGGGATTATTAGAAGAAAGAATGAATTTAAGTCGTCTAAAACTTGCTATCAAATA
  TAACCAGAAAGAGTTTGTCTCCCAGTCTAACTGCCAGCAGTTCCTGAACACTGTTTGGTT
  TGGACAGATGTCGGGTTACCGACGCAAGCCCACCTGTAAGAAGATAATGACTGTTTTGAC
  AGTAGGCATCTTTTGGCCAGTTTTGTCACTTTGTTATTTGATAGCTCCCAAATCTCAGTT
  TGGCAGAATCATTCACACACCTTTTATGAAATTTATCATTCATGGAGCATCATATTTCAC
  ATTTCTGCTGTTGCTTAATCTATACTCTCTTGTCTACAATGAGGATAAGAAAAACACAAT
  GGGGCCAGCCCTTGAAAGAATAGACTATCTTCTTATTCTGTGGATTATTGGGATGATTTG
  GTCAGACATTAAAAGACTCTGGTATGAAGGGTTGGAAGACTTTTTAGAAGAATCTCGTAA
  TCAACTCAGTTTTGTCATGAATTCTCTTTATTTGGCAACCTTTGCCCTCAAAGTGGTTGC
  TCACAACAAGTTTCATGATTTTGCTGATCGGAAGGATTGGGATGCATTCCATCCTACACT
  GGTGGCAGAAGGGCTTTTTGCATTTGCAAATGTTCTAAGTTATCTTCGTCTCTTTTTTAT
  GTATACAACCAGCTCTATCTTGGGTCCATTACAGATTTCAATGGGACAGATGTTACAAGA
  TTTTGGAAAATTTCTTGGGATGTTTCTTCTTGTTTTGTTTTCTTTCACAATTGGACTGAC
  ACAACTGTATGATAAAGGATATACTTCAAAGGAGCAGAAGGACTGTGTAGGCATCTTCTG
  TGAACAGCAAAGCAATGATACCTTCCATTCGTTCATTGGCACCTGCTTTGCTTTGTTCTG
  GTATATTTTCTCCTTAGCGCATGTGGCAATCTTTGTCACAAGATTTAGCTATGGAGAAGA
  ACTGCAGTCCTTTGTGGGAGCTGTCATTGTTGGTACATACAATGTCGTGGTTGTGATTGT
  GCTTACCAAACTGCTGGTGGCAATGCTTCATAAAAGCTTTCAGTTGATAGCAAATCATGA
  AGACAAAGAATGGAAGTTTGCTCGAGCAAAATTATGGCTTAGCTACTTTGATGACAAATG
  TACGTTACCTCCACCTTTCAACATCATTCCCTCACCAAAGACTATCTGCTATATGATTAG
  TAGCCTCAGTAAGTGGATTTGCTCTCATACATCAAAAGGCAAGGTCAAACGGCAAAACAG
  TTTAAAGGAATGGAGGAATTTGAAACAGAAGAGAGATGAAAACTATCAAAAAGTGATGTG
  CTGCCTAGTGCATCGTTACTTGACTTCCATGAGACAGAAGATGCAAAGTACAGATCAGGC
  AACTGTGGAAAATCTAAACGAACTGCGCCAAGATCTGTCAAAATTCCGAAATGAAATAAG
  GGATTTACTTGGCTTTCGGACTTCTAAATATGCTATGTTTTATCCAAGAAATTAACCATT
  TTCTAAATCATGGAGCGAATAATTTTCAATAACAGATCCAAAAGACTATATTGCATAACT
  TGCAATGAAATTAATGAGATATATATTGAAATAAAGAATTATGTAAAAGCCATTCTTTAA
  AATATTTATAGCATAAATATATGTTATGTAAAGTGTGTATATAGAATTAGTTTTTTAAAC
  CTTCTGTTAGTGGCTTTTTGCAGAAGCAAAACAGATTAAGTAGATAGATTTTGTTAGCAT
  GCTGCTTGGTTTTCTTACTTAGTGCTTTAAAATGTTTTTTTTTATGTTTAAGAGGGGCAG
  TTATAAATGGACACATTGCCCAGAATGTTTTGTAAAATGAAGACCAGCAAATGTAGGCTG
  ATCTCCTTCACAGGATACACTTGAAATATAGAAGTTATGTTTTAAATATCTCTGTTTTAG
  GAGTTCACATATAGTTCAGCATTTATTGTTTAGGAGTATAATTTTATTTTATCTAAAATA
  ATAGTCTATTTTTTCTTTTGTATTTTGTTATAATCTTAAGCAACAAAGAAAAAACCCTAA
  TATTTGAATCTATTTATGTCTTTCAATTTAAATTCACTTCAGTTTTTGTTATTGTAATAT
  ATTTACTTTTACATGGTTATAATCACTTTATATTTTTAATGTTTTTTTCACTTAATATTT
  TATATATACATTTCCATGTATTGATGTAGTTAGTCCACATTTAAATTTTTATAGAATTAT
  ATAGTTTTTGAAAAATACAGTCAGTAGATGTTTTATTTTTTAGCTATTCAGTTATGTTTA
  TAAGTTTGCATAGCTACTTCTCGACATTTGGTTTGTTTTAATTTTTTTGTATCATAATAG
  TCCTATTTTTTTTTCAAGTTGGAGTGAATGTTTTTAGTTTTAAGATAGATAGGAGACACT
  TTTTTATCACATGTAGTCACAACCTGTTTTGTTTTTGTAAAACATAGGAAGTCTCTTTAA
  TGCAATGATTTGTTTTATATTTGGACTAAGGTTCTTGAGCTTATCTCCCAAGGTACTTTC
  CATAATTTAACACAGCTTCTATAAAAGTGACTTCATGCTTACTTGTGGATCATTCTTGCT
  GCTTAAGATGAAAAGCATTGGTTTTTTAAAATTAGAGAATAAAATATGTATTTAAATTTT
  TGGTGTGTTCACATAAAGGGATGTAGCTAAAATGTTTTCATAGGCTATTATATATTCTCG
  CAGCATTTCCAGTTAAGAGGATATTAGGTATATAATTCTCTTCTTAACCGAATGTCAGAT
  GGTCTTACGCCACAGGGTGCAGGTAACCCTTGGTCTGTAAGCACCACCGATCCAGGGATC
  ATTGTCTAAATAGGTTACTATTGTTTGTTTCATCTTGCTTTTGCATTTTTATTTTTTAAT
  TTCCAAATTTTAAGTGTTCCCTCTTTGGGGCAAATTCTTATAAAAATGTTTATTGTAAAG
  TTATATATTTTGTCTACGATGGGATTATGCACTTCCCAATTGGGATTTTACATCTGGATT
  TTTAGTCATTCTAAAAAACACCTAATTATTAAAACATTTATAGAGTGCCTACTGTATGCA
  TGAGTTGAGTTGCTTCTGAGGTACATTTTGAATGACAGCATATTGTAAGAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003304 unedited
  CAGATTTTGGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGCCGGTGG
  GGGCCCCGCCCCCGTCTCCTGGCCTGCCCCCTTCAGGGCCGCGATGATGGCGGCCCTGTA
  CCCGAGCACGGACCTCTCGGGCGCCTCCTCCTCCTCCCTGCCTTCCTCTCCATCCTCTTC
  CTCGCCGAACGAGGTGATGGCGCTGAAGGATGTGCGGGAGGTGAAGGAGGAGAATACGCT
  GAATGAGAAGCTTTTCTTGCTGGCGTGCGACAAGGGTGACTATTATATGGTTAAAAAGAT
  TTTGGAGGAAAACAGTTCAGGTGACTTGAACATAAATTGCGTAGATGTGCTTGGGAGAAA
  TGCTGTTACCATAACTATTGAAAACGAAAACTTGGATATACTGCAGCTTCTTTTGGACTA
  CGGTTGTCAGAAACTAATGGAACGAATTCAGAATCCTGAGTATTCAACAACTATGGATGT
  TGCACCTGTCATTTTAGCTGCTCATCGTAACAACTATGAAATTCTTACAATGCTCTTAAA
  ACAGGATGTATCTCTTCCCAAGCCCCATGCAGTTGGGCTGGGAATGCACTTTGTGTTCTG
  CAAAAAACAAAAGGGATAGCCTCCCGCATTTCCGGGTTCGTCTTGGTATATATCCGATGT
  TTGGCCAGGCCCACTTTAAAAAGGTTAACAGAGAGGGATCCCATTTCTGGGGGCATTGGA
  ACTTAGGGCTGATTTAAAACACTAAATCTGGGGGAGGTGGAATTCCGGATGGTTTTATGG
  AACTGGCCCGCGAAGTNAAAAGGTGGGTTAAGGATTTCATTGGCACAGCCCGGAATTTTC
  CGGGATTGGAATTATATTAAACCCATACTCTTTTGAACAGGCCTTTTGAAATACGGGGGT
  TTTATAAAAGAAGAAGGTTTTCAATGCCTTCAAACTGTTTTAATAAAACCCCAAAGGGTT
  G
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003304 unedited
  CGACCCTTGGGCATGGCAACTTGCCAGGNCCAGGAAAGCACTGGGGAGGGTCACAGGATG
  CCACCCGGGATCTGTTCAGAACACTTGACCGCGGCCCAATCAAATCGAGTTTTTTTTTTT
  TTTTTTTTTTTTTATAATCTAATGTCAAATTTTATTCCCCTTTTTTTTCCTTACAATATG
  CTGTCTTTCAAAATGTCCCTCAAAAGCAACTCAACTCTTGCATACAGTAAGCACTCTATA
  AATGTTTTAATAATTAGGCGTTCTTTAAAAAGACTAAAAATCCATATGTAAACTCCCAAT
  TGGGAAGTGCATAATCCCCTCGTACACAAAATATATAACTTCACAATAAACATTTTTATA
  AAACTTTGCCCCAAAGAGGGAACACTTAAAATTTGGAAATTAAAAAATAAAAATGCCAAC
  GCAAGCTGAAACAAACAATTGTAACCTATTTAGACCATGATCCACTGGATCGGTGGTGCT
  TACAGACCAAGGGTTACCTGCTCCCTGTGGCGTAAGACCATCTGACATTCGGTTAAAAAG
  AGAATTATGCACCTAATATCCTCTTAACTGGAAACGCTGCGAGAATATATAATACCCTAT
  GAAAACCTTTTAACTACATCCCTTTATGTGAACACACCAAAAATTTAAACACATATTTTA
  TTCTCTAATTAAAAAAAACCAATGCTTTTCATCTTAAGCCGCGATAATGATCCACAACTA
  ACCATGAAGTCACTTTTATAGAAGCTGTGTTAAATTACTGGAAATACCTCGCGAGATAAG
  CTCAAGAACCTTAGTCCCAATATAAAACAAATCATTGCATGAAAGAAACTTCCTATGTTT
  TAAAAAACAAAACAGGTTGGGACTACCAGTGAAAAAACAGCGCTCTCTCTCTAATCTAAA
  CTATAACATCCCCCCCACTAGAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003304
• Insert Size: 4100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_003304.3.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003304.3, NP_003295.1
• RefSeq Size: 4085 bp
• RefSeq ORF: 2280 bp
• Locus ID: 7220
• Cytogenetics: 3q23
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Protein Pathways: Calcium signaling pathway, Huntington's disease, Parkinson's disease
• Gene Summary: 'The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.