GALK2 (NM_001001556) Human Untagged Clone

CAT#: SC122009

GALK2 (untagged)-Human galactokinase 2 (GALK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALK2
• Synonyms: GK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC122009 sequence for NM_001001556 edited (data generated by NextGen Sequencing)
  ATGCCCGTCCTATATGACAGGTTACTGAAGCTAAAGGAGATGTTTAACTCCAAGTTTGGA
  TCTATTCCCAAGTTTTATGTTCGAGCACCAGGAAGAGTCAACATAATAGGAGAGCATATA
  GATTATTGTGGATATTCTGTTCTTCCTATGGCTGTAGAACAAGATGTGCTAATAGCTGTA
  GAACCTGTGAAAACGTACGCTCTCCAACTGGCCAATACAAATCCCTTGTATCCGGACTTC
  AGTACTAGTGCTAATAACATCCAGATTGATAAAACCAAGCCTTTGTGGCACAACTATTTC
  TTATGTGGACTTAAAGGAATTCAGGAACACTTTGGTCTTAGTAACCTGACTGGAATGAAC
  TGCCTGGTAGATGGAAATATCCCACCAAGTTCTGGCCTCTCCAGCTCCAGTGCTTTGGTC
  TGTTGTGCTGGCTTGGTGACGCTCACAGTGCTGGGAAGGAATCTATCCAAGGTGGAACTT
  GCAGAAATCTGTGCCAAGAGTGAGCGTTACATTGGCACTGAAGGAGGAGGCATGGACCAG
  TCTATATCATTTCTTGCAGAAGAAGGAACTGCCAAGTTGATAGAATTTAGTCCTCTGAGG
  GCAACCGATGTAAAACTCCCAAGTGGAGCAGTGTTTGTGATTGCCAACAGTTGTGTGGAG
  ATGAATAAGGCAGCAACTTCCCATTTCAATATCAGGGTGATGGAGTGTCGGCTGGCTGCG
  AAGCTCCTGGCTAAATACAAAAGCTTGCAATGGGACAAAGTACTGAGGCTGGAGGAGGTG
  CAGGCTAAACTAGGGATTAGTCTAGAAGAAATGCTGTTGGTCACAGAAGATGCCCTTCAT
  CCTGAACCCTATAACCCTGAGGAGATCTGCAGGTGTCTGGGAATTAGCCTGGAGGAACTC
  CGAACCCAAATCCTGAGTCCAAACACTCAAGATGTGCTCATCTTCAAACTCTATCAGCGG
  GCAAAGCATGTGTACAGCGAGGCTGCGCGAGTGCTCCAGTTTAAGAAGATATGTGAAGAA
  GCACCTGAAAACATGGTCCAGCTGCTGGGAGAGTTGATGAACCAGAGCCACATGAGCTGC
  CGGGACATGTATGAGTGCAGCTGCCCCGAGCTGGATCAGCTGGTGGACATCTGTCGGAAG
  TTTGGGGCTCAAGGGTCACGACTTACTGGAGCAGGATGGGGAGGCTGTACAGTATCAATG
  GTACCTGCGGACAAGCTGCCCAGCTTTCTAGCAAATGTGCACAAAGCTTATTACCAGAGG
  AGTGATGGAAGCTTAGCACCGGAGAAGCAAAGTTTGTTTGCTACCAAACCTGGAGGTGGG
  GCTTTGGTTTTGCTTGAGGCCTGA
  
  Clone variation with respect to NM_001001556.1
  1188 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001001556 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGGACATCGTCCGGT
  GCTGCAGGTCTCACCGAAGGCGTCGGAAACTGCGCTCGCATCGAGCAGTTTCCAGCCTCC
  TGGGTAAAGGAGCAGTCTCCTCCCTTGCTTGGGACTCTGGACGCATCTCATTCCGGTGAA
  AGTAAGGGACAGCTTAGGACCAGAAGCCTTTCGCGGAGAAAAGGCTGACATGCCCGTCCT
  ATATGACAGGTTACTGAAGCTAAAGGAGATGTTTAACTCCAAGTTTGGATCTATTCCCAA
  GTTTTATGTTCGAGCACCAGGAAGAGTCAACATAATAGGAGAGCATATAGATTATTGTGG
  ATATTCTGTTCTTCCTATGGCTGTAGAACAAGATGTGCTAATAGCTGTAGAACCTGTGAA
  AACGTACGCTCTCCAACTGGCCAATACAAATCCCTTGTATCCGGACTTCAGTACTAGTGC
  TAATAACATCCAGATTGATAAAACCAAGCCTTTGTGGCACAACTATTTCTTATGTGGACT
  TAAAGGAATTCAGGAACACTTTGGTCTTAGTAACCTGACTGGAATGAACTGCCTGGTAGA
  TGGAAATATCCCACCAAGTTCTGGCCTCTCCAGCTCCAGTGCTTTGGTCTGTTGTGCTGG
  CTTGGTGACGCTCACAGTGCTGGGAAGGAATCTATCCAAGGTGGAACTTGCAGAAATCTG
  TGCCAAGAGTGAGCGTTACATTGGCACTNGAAGGAGANGCATGGACCAGTCTATATCATT
  TCTTGCAGAAGAAAGAACTGCCCAGNTGATAGAATTTAGTCCTCTGAGGGGNCACCGATG
  TAAACTCCCCAGTGGAGCAGTGNNTTGTGATTGCCCCACAGTTGTGTGGAGAATGATAAN
  GGCAGCACTTCCCATTTCATATCAGGNNTGATGNANTGTCGCTGGCTGCAAAACTCTGGC
  TAAATACAAAGCTGCATGGGGACAAGACTGAGGCTGGAAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001001556 unedited
  NGGTCGTACTTGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTAATTGGAACAATATATTTAATTCAAGTCCAATCCATGTAATCAGTATTAAGA
  GTGTGTTTTACCATTCATCTTTAAAATCTTCAAATCAATAAATCTCAGTAAGTTATTCTT
  AAAAGATCTGTCCAAGCATGATCTTGGCTCTTAATAGTAAAACCATATTTTAAGATGGAA
  AAAGAATCATTATGAAGCATAGAGAGCTTTCAACCATTTCTTTGAAAATATATCTTGATA
  TAATAGCAACCGTTCATCATAATACAAAACAGAAGGAAGATTAATTTACTGGGGCACAAA
  AAGTCCTGCCAATTCCTAAGTGCCCTAAGTAGTTTCTCTCAAACTTTTTACATTTTTTCA
  GGCCTCAAGCAAAACCAAAGCCCCACCTCCAGGTTTGGTAGCAAACAAACTTTGCTTCTC
  CGGTGCTAAGCTTCCATCACTCCTCTGGTAATAAGCTTTGTGCACATTTGCTAAAAAGCT
  GGGCAGCTTGTCCGCAGGTACCATTGATACTGTACAGCCTCCCCATCCTGCTCCAGTAAG
  TCGTGACCCTTGAGCCCCAAACTTCCGACAGATGTCCACCAGCTGATCCAGCTCGGGGCA
  GCTGCACTCATACATGTCCCGGCAGCTCATGTGGCTCTGGTTCATCAACTCTCCCAGCAG
  CTGGACCATGTTNTCAGGTGCTTCTTCACATATCTTCTTAAACTGGAGCACTCGCGCAGC
  CTCGCTGTACACATGCTTTTGCCCGCTGATTAGAGTTAGAAGAGAGCACATCTTGAGTGG
  TTNGACTCAGGGATTGGTTTCGAGGTCCTCCAGGCTATTCCCCAGACACTGCAGATCTCT
  CAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001001556
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001556.1, NP_001001556.1
• RefSeq Size: 3208 bp
• RefSeq ORF: 1344 bp
• Locus ID: 2585
• Cytogenetics: 15q21.1-q21.2
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a highly efficient N-acetylgalactosamine (GalNAc) kinase, which has galactokinase activity when galactose is present at high concentrations. The encoded protein is a member of the GHMP kinase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2017]'
  Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The encoded isoform (2) has a shorter and alternate N-terminus, as compared to isoform 1.