CLCN1 (NM_000083) Human Untagged Clone

CAT#: SC122070

CLCN1 (untagged)-Human chloride channel 1, skeletal muscle (CLCN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLCN1
• Synonyms: CLC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000083 edited
  AAGGACAGGGGCAAGCAGGCCAAGGCCTGGCCGGGGCTCGGGGGGAGGGAATATGGAGCA
  ATCCCGGTCACAGCAGCGTGGGGGTGAACAAAGCTGGTGGGGTAGTGACCCCCAGTACCA
  GTATATGCCCTTTGAACACTGCACCAGCTACGGACTGCCCTCTGAGAATGGGGGCCTCCA
  GCACAGGCTCCGGAAGGATGCAGGCCCCCGCCACAACGTCCACCCCACACAGATTTATGG
  CCATCACAAAGAACAATTCTCAGACAGGGAGCAGGACATAGGGATGCCCAAGAAGACAGG
  CTCCAGTTCTACCGTGGACAGCAAGGATGAGGATCACTATTCTAAATGTCAAGATTGTAT
  CCACCGCCTGGGACAGGTGGTGAGAAGAAAATTAGGGGAAGACTGGATCTTTCTGGTGCT
  TCTGGGACTGCTGATGGCTCTGGTCAGCTGGAGCATGGACTACGTCAGTGCCAAAAGCCT
  TCAGGCCTACAAGTGGTCCTACGCGCAGATGCAGCCCAGCCTTCCTCTGCAGTTCCTGGT
  CTGGGTCACCTTCCCACTAGTCCTCATCCTCTTCAGCGCCCTCTTCTGCCACCTCATCTC
  TCCCCAGGCTGTTGGCTCTGGAATCCCCGAAATGAAGACAATACTTCGTGGGGTTGTCCT
  GAAGGAATACCTCACAATGAAAGCCTTTGTGGCCAAGGTTGTCGCCCTGACTGCGGGCCT
  GGGCAGTGGCATCCCCGTGGGGAAAGAGGGCCCCTTCGTCCACATTGCCAGCATCTGTGC
  TGCTGTCCTCAGCAAATTCATGTCTGTGTTCTGCGGGGTATATGAGCAGCCATACTACTA
  CTCTGATATCCTGACGGTGGGCTGTGCTGTGGGAGTCGGCTGTTGTTTTGGGACACCACT
  TGGAGGAGTGCTATTTAGCATCGAGGTCACCTCCACCTACTTTGCTGTTCGGAACTACTG
  GAGAGGATTCTTTGCAGCCACGTTCAGCGCCTTTGTGTTTCGAGTGCTGGCAGTGTGGAA
  CAAGGATGCTGTCACCATCACTGCTCTGTTCAGAACCAATTTCCGAATGGATTTCCCCTT
  TGACCTGAAGGAACTACCAGCTTTTGCTGCCATCGGGATTTGCTGTGGGCTCCTGGGAGC
  TGTATTTGTGTATCTGCATCGCCAAGTCATGCTCGGTGTCCGAAAGCACAAGGCCCTCAG
  CCAGTTTCTTGCTAAGCACCGCCTGCTGTATCCTGGAATTGTTACCTTTGTCATTGCCTC
  ATTCACCTTCCCACCAGGAATGGGTCAATTCATGGCTGGAGAGTTGATGCCCCGCGAAGC
  CATCAGTACTTTGTTTGACAACAATACATGGGTGAAACACGCGGGTGATCCTGAGAGCCT
  GGGCCAGTCAGCTGTGTGGATTCACCCCCGGGTCAACGTTGTCATCATCATCTTTCTCTT
  CTTCGTCATGAAGTTCTGGATGTCCATCGTGGCCACCACTATGCCCATACCCTGCGGAGG
  CTTCATGCCTGTGTTTGTGCTAGGAGCTGCATTTGGAAGGCTGGTAGGAGAAATCATGGC
  CATGCTCTTTCCTGATGGTATTTTGTTTGATGACATCATCTACAAGATCCTACCTGGGGG
  CTATGCAGTAATTGGAGCAGCAGCGCTGACTGGTGCCGTTTCCCACACAGTCTCCACAGC
  TGTGATTTGCTTCGAATTAACGGGTCAGATTGCTCACATCCTGCCCATGATGGTGGCTGT
  TATCTTGGCCAACATGGTGGCCCAGAGCCTGCAGCCCTCTCTCTATGACAGCATCATCCA
  GGTCAAGAAGCTACCCTACTTGCCTGACCTTGGCTGGAACCAGCTCAGCAAATATACCAT
  CTTTGTTGAGGACATCATGGTACGTGATGTGAAGTTTGTTTCAGCTTCTTACACATATGG
  GGAGTTGCGAACCCTGCTCCAGACCACCACAGTCAAGACTTTACCACTGGTTGACTCAAA
  AGATTCAATGATCCTGCTGGGCTCGGTGGAGCGGTCGGAACTGCAGGCCCTCCTGCAGCG
  CCACCTGTGTCCTGAGCGCAGGCTGCGCGCAGCCCAAGAGATGGCGCGGAAGTTGTCGGA
  GCTGCCTTACGACGGGAAGGCGCGGCTGGCTGGGGAGGGGCTCCCCGGCGCGCCTCCAGG
  CCGGCCCGAGTCCTTCGCCTTTGTGGATGAGGATGAGGACGAAGATCTCTCTGGCAAGAG
  CGAGCTTCCTCCTTCCCTTGCTCTCCACCCCTCTACTACTGCCCCTCTGTCCCCAGAAGA
  GCCCAATGGGCCTCTGCCTGGCCACAAACAGCAGCCGGAAGCACCAGAGCCTGCAGGTCA
  AAGACCCTCCATCTTCCAGTCCCTGCTTCACTGCTTGCTGGGCAGAGCTCGCCCCACAAA
  GAAGAAAACAACCCAGGATTCCACAGATTTAGTGGATAACATGTCACCTGAAGAGATTGA
  GGCCTGGGAGCAGGAGCAGCTGAGCCAGCCTGTCTGTTTTGATTCCTGCTGTATTGACCA
  GTCTCCCTTCCAGCTGGTGGAGCAGACAACCCTGCACAAGACTCATACCCTGTTTTCACT
  CCTTGGCCTCCACCTCGCTTACGTGACCAGCATGGGGAAGCTCAGGGGCGTCCTGGCCCT
  GGAGGAGCTACAGAAGGCCATTGAGGGGCACACCAAGTCTGGGGTGCAGCTCCGCCCTCC
  CCTTGCCAGCTTCCGGAACACGACTTCAACTCGAAAGAGTACCGGGGCACCTCCATCTTC
  TGCAGAGAACTGGAACCTGCCTGAGGACAGGCCTGGGGCCACTGGAACAGGGGATGTGAT
  TGCTGCCTCCCCAGAGACCCCTGTGCCATCTCCTTCCCCAGAGCCCCCTCTCTCCCTGGC
  CCCAGGCAAGGTAGAGGGCGAGTTGGAGGAGCTGGAGCTGGTGGAGAGTCCAGGGCTGGA
  AGAGGAGCTGGCCGACATCTTGCAGGGCCCCAGCCTGCGATCCACAGACGAGGAGGATGA
  GGATGAACTGATCCTTTGACCCCCTCCCACGACCTCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_000083
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000083.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000083.1, NP_000074.1
• RefSeq Size: 3093 bp
• RefSeq ORF: 3093 bp
• Locus ID: 1180
• Cytogenetics: 7q34
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: 'The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]'
  Transcript Variant: This variant (1) encodes the functional protein.