CYBB (NM_000397) Human Untagged Clone

CAT#: SC122091

CYBB (untagged)-Human cytochrome b-245, beta polypeptide (CYBB)


  "NM_000397" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "CYBB"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CYBB
Synonyms AMCBX2; CGD; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC122091 sequence for NM_000397 edited (data generated by NextGen Sequencing)
ATGGGGAACTGGGCTGTGAATGAGGGGCTCTCCATTTTTGTCATTCTGGTTTGGCTGGGG
TTGAACGTCTTCCTCTTTGTCTGGTATTACCGGGTTTATGATATTCCACCTAAGTTCTTT
TACACAAGAAAACTTCTTGGGTCAGCACTGGCACTGGCCAGGGCCCCTGCAGCCTGCCTG
AATTTCAACTGCATGCTGATTCTCTTGCCAGTCTGTCGAAATCTGCTGTCCTTCCTCAGG
GGTTCCAGTGCGTGCTGCTCAACAAGAGTTCGAAGACAACTGGACAGGAATCTCACCTTT
CATAAAATGGTGGCATGGATGATTGCACTTCACTCTGCGATTCACACCATTGCACATCTA
TTTAATGTGGAATGGTGTGTGAATGCCCGAGTCAATAATTCTGATCCTTATTCAGTAGCA
CTCTCTGAACTTGGAGACAGGCAAAATGAAAGTTATCTCAATTTTGCTCGAAAGAGAATA
AAGAACCCTGAAGGAGGCCTGTACCTGGCTGTGACCCTGTTGGCAGGCATCACTGGAGTT
GTCATCACGCTGTGCCTCATATTAATTATCACTTCCTCCACCAAAACCATCCGGAGGTCT
TACTTTGAAGTCTTTTGGTACACACATCATCTCTTTGTGATCTTCTTCATTGGCCTTGCC
ATCCATGGAGCTGAACGAATTGTACGTGGGCAGACCGCAGAGAGTTTGGCTGTGCATAAT
ATAACAGTTTGTGAACAAAAAATCTCAGAATGGGGAAAAATAAAGGAATGCCCAATCCCT
CAGTTTGCTGGAAACCCTCCTATGACTTGGAAATGGATAGTGGGTCCCATGTTTCTGTAT
CTCTGTGAGAGGTTGGTGCGGTTTTGGCGATCTCAACAGAAGGTGGTCATCACCAAGGTG
GTCACTCACCCTTTCAAAACCATCGAGCTACAGATGAAGAAGAAGGGGTTCAAAATGGAA
GTGGGACAATACATTTTTGTCAAGTGCCCAAAGGTGTCCAAGCTGGAGTGGCACCCTTTT
ACACTGACATCCGCCCCTGAGGAAGACTTCTTTAGTATCCATATCCGCATCGTTGGGGAC
TGGACAGAGGGGCTGTTCAATGCTTGTGGCTGTGATAAGCAGGAGTTTCAAGATGCGTGG
AAACTACCTAAGATAGCGGTTGATGGGCCCTTTGGCACTGCCAGTGAAGATGTGTTCAGC
TATGAGGTGGTGATGTTAGTGGGAGCAGGGATTGGGGTCACACCCTTCGCATCCATTCTC
AAGTCAGTCTGGTACAAATATTGCAATAACGCCACCAATCTGAAGCTCAAAAAGATCTAC
TTCTACTGGCTGTGCCGGGACACACATGCCTTTGAGTGGTTTGCAGATCTGCTGCAACTG
CTGGAGAGCCAGATGCAGGAAAGGAACAATGCCGGCTTCCTCAGCTACAACATCTACCTC
ACTGGCTGGGATGAGTCTCAGGCCAATCACTTTGCTGTGCACCATGATGAGGAGAAAGAT
GTGATCACAGGCCTGAAACAAAAGACTTTGTATGGACGGCCCAACTGGGATAATGAATTC
AAGACAATTGCAAGTCAACACCCTAATACCAGAATAGGAGTTTTCCTCTGTGGACCTGAA
GCCTTGGCTGAAACCCTGAGTAAACAAAGCATCTCCAACTCTGAGTCTGGCCCTCGGGGA
GTGCATTTCATTTTCAACAAGGAAAACTTCTAA

Clone variation with respect to NM_000397.3
>OriGene 5' read for NM_000397 unedited
NNNCCGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGTCAACC
TCTGCCACCATGGGGAACTGGGCTGTGAATGAGGGGCTCTCCATTTTTGTCATTCTGGTT
TGGCTGGGGTTGAACGTCTTCCTCTTTGTCTGGTATTACCGGGTTTATGATATTCCACCT
AAGTTCTTTTACACAAGAAAACTTCTTGGGTCAGCACTGGCACTGGCCAGGGCCCCTGCA
GCCTGCCTGAATTTCAACTGCATGCTGATTCTCTTGCCAGTCTGTCGAAATCTGCTGTCC
TTCCTCAGGGGTTCCAGTGCGTGCTGCTCAACAAGAGTTCGAAGACAACTGGACAGGAAT
CTCACCTTTCATAAAATGGTGGCATGGATGATTGCACTTCACTCTGCGATTCACACCATT
GCACATCTATTTAATGTGGAATGGTGTGTGAATGCCCGAGTCAATAATTCTGATCCTTAT
TCAGTAGCACTCTCTGAACTTGGAGACAGGCAAAATGAAAGTTATCTCAATTTTGCTCGA
AAGAGAATAAAGAACCCTGAAGGAGGCCTGTACCTGGCTGTGACCCTGTTGGCAGGCATC
ACTGGAGTTGTCATCACGCTGTGCCTCATATTAATTATCACTTCCTCCACCAAAACCATC
CGGAGGTCTTACTTTGAAGTCTTTTGGTACACACATCATCTCTTTGTGATCTTCTTCATT
GGCCTTGCCATCCATGGAGCTGAACGAATTGTACGTGGGCAGACCGCAGAGAGTTTTGGC
TGTGCATAATATAACAGTTTGTGAACAAAAATCTCAGATGGGGAANAATAAGGATGCCCA
TCCCTCAGTTGCTGGAACCCTCTATACTNGAATGATATAGTGGTCCATGGTTTGT
>OriGene 3' read for NM_000397 unedited
CTTAAATTAGCCTGCTTTCTTTTATAGCACAGGAAGCAGGACTAGCATGAGCCATAGTCA
TGGCAGGAGGTTAATTCTCCACCTCCAACCCTCTTTTTTCATGCTTCAAATAAGAATGTG
CAGAACCCTTCAGGCAATCCTATTGGTTAGTATCCTTAGCCTGTTCTTGACCCTAGGGAG
AATCAGATTCCTTTGGTCTATGACAGTAATAGAAGTTATTTCTTTAGATAAATTTTGTTT
AAGGCTTTAAATTAATTCTATCTTGGCATTGTGTGGTTCTAAATTCATTCATTCATTTAA
CAAAACTTACTTGAGTATCCGCTCTCTGCCAAGCACAGTGGTAAATGATGAGTATTACAT
TAACTCCAATCAATCCAATATTCTTCTGTAGAAAGAACATCTGGCCCAGTTACCCTGCTG
TATTAGTAAACTGGAGTATGCTCAGATGTCAATACTGTCACTAATAAGTGTTGAGACACT
CTCAACATGTATTATGTCCTAATGATCTGGAAAATTTCCTTCAGTATGATAATAATGATG
GCAAAAATGACACANAGTAGAAATATATAGCTACTCCTTGGTTTCCTTCTTCTTTTGGGG
AATATAAAAACTGATTTTGAGGATTCTGAGGCAGCTCAGTATTGTGGAAAGAGTATCAAA
CCTGGAGTAAGATGGGCTAGATCTGGTCACAGTTTCATAATCTTANGGAAGTTACTGAAA
CCTTTGATCCTTTCCCTTTTGATTCTAAAATTGAGTCCAAGAAATCTTTTCCCTACTTCA
CTGACATTACTGAGAGGAAAAAATGAAATACTGCTTGAAAAGTGCTCTCAAAACATAGAG
CTCCCATAAATGTAACTTATCACTATCAAACAATCTTTGACATTCCNTTNAGGGGAAACC
CATACATNATAGTTTCTTTTTGGTCCTTTTTAAAC
Restriction Sites NotI-NotI     
ACCN NM_000397
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000397.2, NP_000388.2
RefSeq Size 4266 bp
RefSeq ORF 1713 bp
Locus ID 1536
Cytogenetics Xp21.1-p11.4
Domains Ferric_reduct
Protein Families Druggable Genome, Ion Channels: Other, Transmembrane
Protein Pathways Leukocyte transendothelial migration
Gene Summary 'Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]'

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.