CYBB (NM_000397) Human Untagged Clone

CAT#: SC122091

CYBB (untagged)-Human cytochrome b-245, beta polypeptide (CYBB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYBB
• Synonyms: AMCBX2; CGD; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC122091 sequence for NM_000397 edited (data generated by NextGen Sequencing)
  ATGGGGAACTGGGCTGTGAATGAGGGGCTCTCCATTTTTGTCATTCTGGTTTGGCTGGGG
  TTGAACGTCTTCCTCTTTGTCTGGTATTACCGGGTTTATGATATTCCACCTAAGTTCTTT
  TACACAAGAAAACTTCTTGGGTCAGCACTGGCACTGGCCAGGGCCCCTGCAGCCTGCCTG
  AATTTCAACTGCATGCTGATTCTCTTGCCAGTCTGTCGAAATCTGCTGTCCTTCCTCAGG
  GGTTCCAGTGCGTGCTGCTCAACAAGAGTTCGAAGACAACTGGACAGGAATCTCACCTTT
  CATAAAATGGTGGCATGGATGATTGCACTTCACTCTGCGATTCACACCATTGCACATCTA
  TTTAATGTGGAATGGTGTGTGAATGCCCGAGTCAATAATTCTGATCCTTATTCAGTAGCA
  CTCTCTGAACTTGGAGACAGGCAAAATGAAAGTTATCTCAATTTTGCTCGAAAGAGAATA
  AAGAACCCTGAAGGAGGCCTGTACCTGGCTGTGACCCTGTTGGCAGGCATCACTGGAGTT
  GTCATCACGCTGTGCCTCATATTAATTATCACTTCCTCCACCAAAACCATCCGGAGGTCT
  TACTTTGAAGTCTTTTGGTACACACATCATCTCTTTGTGATCTTCTTCATTGGCCTTGCC
  ATCCATGGAGCTGAACGAATTGTACGTGGGCAGACCGCAGAGAGTTTGGCTGTGCATAAT
  ATAACAGTTTGTGAACAAAAAATCTCAGAATGGGGAAAAATAAAGGAATGCCCAATCCCT
  CAGTTTGCTGGAAACCCTCCTATGACTTGGAAATGGATAGTGGGTCCCATGTTTCTGTAT
  CTCTGTGAGAGGTTGGTGCGGTTTTGGCGATCTCAACAGAAGGTGGTCATCACCAAGGTG
  GTCACTCACCCTTTCAAAACCATCGAGCTACAGATGAAGAAGAAGGGGTTCAAAATGGAA
  GTGGGACAATACATTTTTGTCAAGTGCCCAAAGGTGTCCAAGCTGGAGTGGCACCCTTTT
  ACACTGACATCCGCCCCTGAGGAAGACTTCTTTAGTATCCATATCCGCATCGTTGGGGAC
  TGGACAGAGGGGCTGTTCAATGCTTGTGGCTGTGATAAGCAGGAGTTTCAAGATGCGTGG
  AAACTACCTAAGATAGCGGTTGATGGGCCCTTTGGCACTGCCAGTGAAGATGTGTTCAGC
  TATGAGGTGGTGATGTTAGTGGGAGCAGGGATTGGGGTCACACCCTTCGCATCCATTCTC
  AAGTCAGTCTGGTACAAATATTGCAATAACGCCACCAATCTGAAGCTCAAAAAGATCTAC
  TTCTACTGGCTGTGCCGGGACACACATGCCTTTGAGTGGTTTGCAGATCTGCTGCAACTG
  CTGGAGAGCCAGATGCAGGAAAGGAACAATGCCGGCTTCCTCAGCTACAACATCTACCTC
  ACTGGCTGGGATGAGTCTCAGGCCAATCACTTTGCTGTGCACCATGATGAGGAGAAAGAT
  GTGATCACAGGCCTGAAACAAAAGACTTTGTATGGACGGCCCAACTGGGATAATGAATTC
  AAGACAATTGCAAGTCAACACCCTAATACCAGAATAGGAGTTTTCCTCTGTGGACCTGAA
  GCCTTGGCTGAAACCCTGAGTAAACAAAGCATCTCCAACTCTGAGTCTGGCCCTCGGGGA
  GTGCATTTCATTTTCAACAAGGAAAACTTCTAA
  
  Clone variation with respect to NM_000397.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000397 unedited
  NNNCCGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGTCAACC
  TCTGCCACCATGGGGAACTGGGCTGTGAATGAGGGGCTCTCCATTTTTGTCATTCTGGTT
  TGGCTGGGGTTGAACGTCTTCCTCTTTGTCTGGTATTACCGGGTTTATGATATTCCACCT
  AAGTTCTTTTACACAAGAAAACTTCTTGGGTCAGCACTGGCACTGGCCAGGGCCCCTGCA
  GCCTGCCTGAATTTCAACTGCATGCTGATTCTCTTGCCAGTCTGTCGAAATCTGCTGTCC
  TTCCTCAGGGGTTCCAGTGCGTGCTGCTCAACAAGAGTTCGAAGACAACTGGACAGGAAT
  CTCACCTTTCATAAAATGGTGGCATGGATGATTGCACTTCACTCTGCGATTCACACCATT
  GCACATCTATTTAATGTGGAATGGTGTGTGAATGCCCGAGTCAATAATTCTGATCCTTAT
  TCAGTAGCACTCTCTGAACTTGGAGACAGGCAAAATGAAAGTTATCTCAATTTTGCTCGA
  AAGAGAATAAAGAACCCTGAAGGAGGCCTGTACCTGGCTGTGACCCTGTTGGCAGGCATC
  ACTGGAGTTGTCATCACGCTGTGCCTCATATTAATTATCACTTCCTCCACCAAAACCATC
  CGGAGGTCTTACTTTGAAGTCTTTTGGTACACACATCATCTCTTTGTGATCTTCTTCATT
  GGCCTTGCCATCCATGGAGCTGAACGAATTGTACGTGGGCAGACCGCAGAGAGTTTTGGC
  TGTGCATAATATAACAGTTTGTGAACAAAAATCTCAGATGGGGAANAATAAGGATGCCCA
  TCCCTCAGTTGCTGGAACCCTCTATACTNGAATGATATAGTGGTCCATGGTTTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000397 unedited
  CTTAAATTAGCCTGCTTTCTTTTATAGCACAGGAAGCAGGACTAGCATGAGCCATAGTCA
  TGGCAGGAGGTTAATTCTCCACCTCCAACCCTCTTTTTTCATGCTTCAAATAAGAATGTG
  CAGAACCCTTCAGGCAATCCTATTGGTTAGTATCCTTAGCCTGTTCTTGACCCTAGGGAG
  AATCAGATTCCTTTGGTCTATGACAGTAATAGAAGTTATTTCTTTAGATAAATTTTGTTT
  AAGGCTTTAAATTAATTCTATCTTGGCATTGTGTGGTTCTAAATTCATTCATTCATTTAA
  CAAAACTTACTTGAGTATCCGCTCTCTGCCAAGCACAGTGGTAAATGATGAGTATTACAT
  TAACTCCAATCAATCCAATATTCTTCTGTAGAAAGAACATCTGGCCCAGTTACCCTGCTG
  TATTAGTAAACTGGAGTATGCTCAGATGTCAATACTGTCACTAATAAGTGTTGAGACACT
  CTCAACATGTATTATGTCCTAATGATCTGGAAAATTTCCTTCAGTATGATAATAATGATG
  GCAAAAATGACACANAGTAGAAATATATAGCTACTCCTTGGTTTCCTTCTTCTTTTGGGG
  AATATAAAAACTGATTTTGAGGATTCTGAGGCAGCTCAGTATTGTGGAAAGAGTATCAAA
  CCTGGAGTAAGATGGGCTAGATCTGGTCACAGTTTCATAATCTTANGGAAGTTACTGAAA
  CCTTTGATCCTTTCCCTTTTGATTCTAAAATTGAGTCCAAGAAATCTTTTCCCTACTTCA
  CTGACATTACTGAGAGGAAAAAATGAAATACTGCTTGAAAAGTGCTCTCAAAACATAGAG
  CTCCCATAAATGTAACTTATCACTATCAAACAATCTTTGACATTCCNTTNAGGGGAAACC
  CATACATNATAGTTTCTTTTTGGTCCTTTTTAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000397
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000397.2, NP_000388.2
• RefSeq Size: 4266 bp
• RefSeq ORF: 1713 bp
• Locus ID: 1536
• Cytogenetics: Xp21.1-p11.4
• Domains: Ferric_reduct
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: 'Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]'