KCNA1 (NM_000217) Human Untagged Clone

CAT#: SC122128

KCNA1 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia) (KCNA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNA1
• Synonyms: AEMK; EA1; HBK1; HUK1; KV1.1; MBK1; MK1; RBK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000217 edited
  ATGACGGTGATGTCTGGGGAGAACGTGGACGAGGCTTCGGCCGCCCCGGGCCACCCCCAG
  GATGGCAGCTACCCCCGGCAGGCCGACCACGACGACCACGAGTGCTGCGAGCGCGTGGTG
  ATCAACATCTCCGGGCTGCGCTTCGAGACGCAGCTCAAGACCCTGGCGCAGTTCCCCAAC
  ACGCTGCTGGGCAACCCTAAGAAACGCATGCGCTACTTCGACCCCCTGAGGAACGAGTAC
  TTCTTCGACCGCAACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCCGGCGGC
  CGCCTGCGGAGGCCGGTCAACGTGCCCCTGGACATGTTCTCCGAGGAGATCAAGTTTTAC
  GAGTTGGGCGAGGAGGCCATGGAGAAGTTCCGGGAGGACGAGGGCTTCATCAAGGAGGAG
  GAGCGCCCTCTGCCCGAGAAGGAGTACCAGCGCCAGGTGTGGCTGCTCTTCGAGTACCCC
  GAGAGCTCGGGGCCCGCCAGGGTCATCGCCATCGTCTCCGTCATGGTCATCCTCATCTCC
  ATCGTCATCTTTTGCCTGGAGACGCTCCCCGAGCTGAAGGATGACAAGGACTTCACGGGC
  ACCGTCCACCGCATCGACAACACCACGGTCATCTACAATTCCAACATCTTCACAGACCCC
  TTCTTCATCGTGGAAACGCTGTGCATCATCTGGTTCTCCTTCGAGCTGGTGGTGCGCTTC
  TTCGCCTGCCCCAGCAAGACGGACTTCTTCAAAAACATCATGAACTTCATAGACATTGTG
  GCCATCATTCCTTATTTCATCACCCTGGGCACCGAGATAGCTGAGCAGGAAGGAAACCAG
  AAGGGCGAGCAGGCCACCTCCCTGGCCATCCTCAGGGTCATCCGCTTGGTAAGGGTTTTT
  AGAATCTTCAAGCTCTCCCGCCACTCTAAGGGCCTCCAGATCCTGGGCCAGACCCTCAAA
  GCTAGTATGAGAGAGCTAGGGCTGCTCATCTTTTTCCTCTTCATCGGGGTCATCCTGTTT
  TCTAGTGCAGTGTACTTTGCCGAGGCGGAAGAAGCTGAGTCGCACTTCTCCAGTATCCCC
  GATGCTTTCTGGTGGGCGGTGGTGTCCATGACCACTGTAGGATACGGTGACATGTACCCT
  GTGACAATTGGAGGCAAGATCGTGGGCTCCTTGTGTGCCATCGCTGGTGTGCTAACAATT
  GCCCTGCCCGTACCTGTCATTGTGTCCAATTTCAACTATTTCTACCACCGAGAAACTGAG
  GGGGAAGAGCAGGCTCAGTTGCTCCACGTCAGTTCCCCTAACTTAGCCTCTGACAGTGAC
  CTCAGTCGCCGCAGTTCCTCTACTATGAGCAAGTCTGAGTACATGGAGATCGAAGAGGAT
  ATGAATAATAGCATAGCCCATTATAGACAGGTCAATATCAGAACTGCCAATTGCACCACA
  GCTAACCAAAACTGCGTTAATAAGAGCAAGCTACTGACCAAAGGGCGAATGACGGTGATG
  TCTGGGGAGAACGTGGACGAGGCTTCGGCCGCCCCGGGCCACCCCCAGGATGGCAGCTAC
  CCCCGGCAGGCCGACCACGACGACCACGAGTGCTGCGAGCGCGTGGTGATCAACATCTCC
  GGGCTGCGCTTCGAGACGCAGCTCAAGACCCTGGCGCAGTTCCCCAACACGCTGCTGGGC
  AACCCTAAGAAACGCATGCGCTACTTCGACCCCCTGAGGAACGAGTACTTCTTCGACCGC
  AACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCCGGCGGCCGCCTGCGGAGG
  CCGGTCAACGTGCCCCTGGACATGTTCTCCGAGGAGATCAAGTTTTACGAGTTGGGCGAG
  GAGGCCATGGAGAAGTTCCGGGAGGACGAGGGCTTCATCAAGGAGGAGGAGCGCCCTCTG
  CCCGAGAAGGAGTACCAGCGCCAGGTGTGGCTGCTCTTCGAGTACCCCGAGAGCTCGGGG
  CCCGCCAGGGTCATCGCCATCGTCTCCGTCATGGTCATCCTCATCTCCATCGTCATCTTT
  TGCCTGGAGACGCTCCCCGAGCTGAAGGATGACAAGGACTTCACGGGCACCGTCCACCGC
  ATCGACAACACCACGGTCATCTACAATTCCAACATCTTCACAGACCCCTTCTTCATCGTG
  GAAACGCTGTGCATCATCTGGTTCTCCTTCGAGCTGGTGGTGCGCTTCTTCGCCTGCCCC
  AGCAAGACGGACTTCTTCAAAAACATCATGAACTTCATAGACATTGTGGCCATCATTCCT
  TATTTCATCACCCTGGGCACCGAGATAGCTGAGCAGGAAGGAAACCAGAAGGGCGAGCAG
  GCCACCTCCCTGGCCATCCTCAGGGTCATCCGCTTGGTAAGGGTTTTTAGAATCTTCAAG
  CTCTCCCGCCACTCTAAGGGCCTCCAGATCCTGGGCCAGACCCTCAAAGCTAGTATGAGA
  GAGCTAGGGCTGCTCATCTTTTTCCTCTTCATCGGGGTCATCCTGTTTTCTAGTGCAGTG
  TACTTTGCCGAGGCGGAAGAAGCTGAGTCGCACTTCTCCAGTATCCCCGATGCTTTCTGG
  TGGGCGGTGGTGTCCATGACCACTGTAGGATACGGTGACATGTACCCTGTGACAATTGGA
  GGCAAGATCGTGGGCTCCTTGTGTGCCATCGCTGGTGTGCTAACAATTGCCCTGCCCGTA
  CCTGTCATTGTGTCCAATTTCAACTATTTCTACCACCGAGAAACTGAGGGGGAAGAGCAG
  GCTCAGTTGCTCCACGTCAGTTCCCCTAACTTAGCCTCTGACAGTGACCTCAGTCGCCGC
  AGTTCCTCTACTATGAGCAAGTCTGAGTACATGGAGATCGAAGAGGATATGAATAATAGC
  ATAGCCCATTATAGACAGGTCAATATCAGAACTGCCAATTGCACCACAGCTAACCAAAAC
  TGCGTTAATAAGAGCAAGCTACTGACCAAAGGGCGAATGACGGTGATGTCTGGGGAGAAC
  GTGGACGAGGCTTCGGCCGCCCCGGGCCACCCCCAGGATGGCAGCTACCCCCGGCAGGCC
  GACCACGACGACCACGAGTGCTGCGAGCGCGTGGTGATCAACATCTCCGGGCTGCGCTTC
  GAGACGCAGCTCAAGACCCTGGCGCAGTTCCCCAACACGCTGCTGGGCAACCCTAAGAAA
  CGCATGCGCTACTTCGACCCCCTGAGGAACGAGTACTTCTTCGACCGCAACCGGCCCAGC
  TTCGACGCCATCCTCTACTACTACCAGTCCGGCGGCCGCCTGCGGAGGCCGGTCAACGTG
  CCCCTGGACATGTTCTCCGAGGAGATCAAGTTTTACGAGTTGGGCGAGGAGGCCATGGAG
  AAGTTCCGGGAGGACGAGGGCTTCATCAAGGAGGAGGAGCGCCCTCTGCCCGAGAAGGAG
  TACCAGCGCCAGGTGTGGCTGCTCTTCGAGTACCCCGAGAGCTCGGGGCCCGCCAGGGTC
  ATCGCCATCGTCTCCGTCATGGTCATCCTCATCTCCATCGTCATCTTTTGCCTGGAGACG
  CTCCCCGAGCTGAAGGATGACAAGGACTTCACGGGCACCGTCCACCGCATCGACAACACC
  ACGGTCATCTACAATTCCAACATCTTCACAGACCCCTTCTTCATCGTGGAAACGCTGTGC
  ATCATCTGGTTCTCCTTCGAGCTGGTGGTGCGCTTCTTCGCCTGCCCCAGCAAGACGGAC
  TTCTTCAAAAACATCATGAACTTCATAGACATTGTGGCCATCATTCCTTATTTCATCACC
  CTGGGCACCGAGATAGCTGAGCAGGAAGGAAACCAGAAGGGCGAGCAGGCCACCTCCCTG
  GCCATCCTCAGGGTCATCCGCTTGGTAAGGGTTTTTAGAATCTTCAAGCTCTCCCGCCAC
  TCTAAGGGCCTCCAGATCCTGGGCCAGACCCTCAAAGCTAGTATGAGAGAGCTAGGGCTG
  CTCATCTTTTTCCTCTTCATCGGGGTCATCCTGTTTTCTAGTGCAGTGTACTTTGCCGAG
  GCGGAAGAAGCTGAGTCGCACTTCTCCAGTATCCCCGATGCTTTCTGGTGGGCGGTGGTG
  TCCATGACCACTGTAGGATACGGTGACATGTACCCTGTGACAATTGGAGGCAAGATCGTG
  GGCTCCTTGTGTGCCATCGCTGGTGTGCTAACAATTGCCCTGCCCGTACCTGTCATTGTG
  TCCAATTTCAACTATTTCTACCACCGAGAAACTGAGGGGGAAGAGCAGGCTCAGTTGCTC
  CACGTCAGTTCCCCTAACTTAGCCTCTGACAGTGACCTCAGTCGCCGCAGTTCCTCTACT
  ATGAGCAAGTCTGAGTACATGGAGATCGAAGAGGATATGAATAATAGCATAGCCCATTAT
  AGACAGGTCAATATCAGAACTGCCAATTGCACCACAGCTAACCAAAACTGCGTTAATAAG
  AGCAAGCTACTGACCAAAGGGCGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000217 unedited
  GTTTTCATTTGTATACGACTCCTATAGGCGGCACGCGATTCGCCCTTATGACGGTGATGT
  CTGGGGAGAACGTGGACGAGGCTTCGGCCGCCCCGGGCCACCCCCAGGATGGCAGCTACC
  CCCGGCAGGCCGACCACGACGACCACGAGTGCTGCGAGCGCGTGGTGATCAACATCTCCG
  GGCTGCGCTTCGAGACGCAGCTCAAGACCCTGGCGCAGTTCCCCAACACGCTGCTGGGCA
  ACCCTAAGAAACGCATGCGCTACTTCGACCCCCTGAGGAACGAGTACTTCTTCGACNCGC
  AACCGGCCCAGCTTCGACGCCATCCTCTACTACTACCAGTCCGGCGGCCGCCTGCGGAGG
  CCGGTCAACGTGCCCCTGGACATGTTCTCCGAGGAGATCAAGTTTTACGAGTTGGGCGAG
  GAGGCCATGGAGAAGTTCCGGGAGGACGAGGGCTTCATCAAGGAGGAGGAGCGCCCTCTG
  CCCGAGAAGGAGTACCAGCGCCAGGTGTGGCTGCTCTTCGAGTACCCCGAGAGCTCGGGG
  CCCGCCAGGGTCATCGCCATCGTCTCCGTCATGGTCATCCTCATCTCCATCGTCATCTTT
  TGCCTGGAGACGCTCCCCGAGCTGAAGGATGACAAGGACTTCACGGGCACCGTCCACCGC
  ATCGACAACACCACGGTCATCTACAATTCCAACATCTTCACAGACCCCTTCTTCATCGTG
  GAAACGCTGTGCATCATCTGGTTCTCCTTCGAGCTGGTGGTGCGCTTCTTCGCCTGCCCC
  AGCAGACAGACTTCTTCAAAACATCATGAACTTCATAGACATTGTGGCCATCATTCCTTA
  TTTCATCACCCTGGGCACCGAGATAGCTGAGCAGGAAGGAAACCAGATGGCGAGCAGGCC
  ACTNCCTGCCATCCTCAGGNTCATCGCTGGTAAGGNCTTTTAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000217
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000217.1, NP_000208.1
• RefSeq Size: 1488 bp
• RefSeq ORF: 1488 bp
• Locus ID: 3736
• Cytogenetics: 12p13.32
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'This gene encodes a voltage-gated delayed potassium channel that is phylogenetically related to the Drosophila Shaker channel. The encoded protein has six putative transmembrane segments (S1-S6), and the loop between S5 and S6 forms the pore and contains the conserved selectivity filter motif (GYGD). The functional channel is a homotetramer. The N-terminus of the channel is associated with beta subunits that can modify the inactivation properties of the channel as well as affect expression levels. The C-terminus of the channel is complexed to a PDZ domain protein that is responsible for channel targeting. Mutations in this gene have been associated with myokymia with periodic ataxia (AEMK). [provided by RefSeq, Jul 2008]'