CHRNA5 (NM_000745) Human Untagged Clone

CAT#: SC122164

CHRNA5 (untagged)-Human cholinergic receptor, nicotinic, alpha 5 (CHRNA5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRNA5
• Synonyms: LNCR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC122164 sequence for NM_000745 edited (data generated by NextGen Sequencing)
  ATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTCCAGCTGGTC
  GCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTATCTGAACCT
  TCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGACTACGAAAGA
  TGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGACTTGCAATA
  TCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTCTGGTTGAAA
  CAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGAATAAAAGTT
  ATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGATAATGCAGAT
  GGACGTTTTGAAGGGACCAGTACGAAAACAGTCATCAGGTACAATGGCACTGTCACCTGG
  ACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTCCCATTTGAC
  CTTCAGAACTGTTCCATGAAATTTGGTTCTTGGACTTATGATGGATCACAGGTTGATATA
  ATTCTAGAGGACCAAGATGTAGACAAGAGAGATTTTTTTGATAATGGAGAATGGGAGATT
  GTGAGTGCAACAGGGAGCAAAGGAAACAGAACCGACAGCTGTTGCTGGTATCCGTATGTC
  ACTTACTCATTTGTAATCAAGCGCCTGCCTCTCTTTTATACCTTGTTCCTTATAATACCC
  TGTATTGGGCTCTCATTTTTAACTGTACTTGTCTTCTATCTTCCTTCAAATGAAGGTGAA
  AAGATTTGTCTCTGCACTTCAGTACTTGTGTCTTTGACTGTCTTCCTTCTGGTTATTGAA
  GAGATCATACCATCATCTTCAAAAGTCATACCTCTAATTGGAGAGTATCTGGTATTTACC
  ATGATTTTTGTGACACTGTCAATTATGGTAACCGTCTTCGCTATCAACATTCATCATCGT
  TCTTCCTCAACACATAATGCCATGGCGCCTTTGGTCCGCAAGATATTTCTTCACACGCTT
  CCCAAACTGCTTTGCATGAGAAGTCATGTAGACAGGTACTTCACTCAGAAAGAGGAAACT
  GAGAGTGGTAGTGGACCAAAATCTTCTAGAAACACATTGGAAGCTGCGCTCGATTCTATT
  CGCTACATTACAAGACACATCATGAAGGAAAATGATGTCCGTGAGGTTGTTGAAGATTGG
  AAATTCATAGCCCAGGTTCTTGATCGGATGTTTCTGTGGACTTTTCTTTTCGTTTCAATT
  GTTGGATCTCTTGGGCTTTTTGTTCCTGTTATTTATAAATGGGCAAATATATTAATACCA
  GTTCAAATTGGAAATGCAAATAAGTGA
  
  Clone variation with respect to NM_000745.3
  1386 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000745 unedited
  TATAGGCGGCCGCGAATTCGGCACGAGGCTGCTGTCCCGGCGGGAGCTGTGGCGCGGAGC
  GGCCCCTCTGCTGCGTCTGCCCTCGTTTTGTCTCACGACTCACACTCAGTGCTCCATTCC
  CCAAGAGTTCGCGTTCCCCGCGCGGCGGTCGAGAGGCGGCTGCCCGCGGTCCCGCGCGGG
  CGCGGGGCGATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTC
  CAGCTGGTCGCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTA
  TCTGAACCTTCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGAC
  TACGAAAGATGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGA
  CTTGCAATATCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTC
  TGGTTGAAACAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGA
  ATAAAAGTTATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGAT
  AATGCAGATGGACGTTTTGAAGGGACCAGTACGAAAACAGTCATCAGGTACAATGGCACT
  GTCACCTGGACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTT
  CCATTTGACCTTCAGAACTGTTCCATGANATTTGGTTCTTGGACTTATGATGGATCACAG
  GTTGATATATTNCTAGAGGACAAGATGTAGACCAGAGAGATTTTTTTGATATGGAGATGG
  GNAGATTGTGAGTGCACAGGGAGCAAAGGAACAGACCGNCAGCTGTGGCTGGTTTCGNAT
  GTCCT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_000745 unedited
  AATTGGGGCCTTAGTTCCGGTTTATTTAACCCAGGTTCAGGAATATTAATACCAGTCTCA
  AATTGGNAAATGCAAATAATTGAAGCCTCCCAGGGNACTGAAGTATATACATTTAGTTAA
  CACACATATATCTGATGGCACCTATAAAATTATGAAAATGTAAGTTATGTGTTAAATTTA
  GTGCAAGCTTTAACAGACTAAGTTGCTAACCTCAATTTATGTTAACAGATTATCCATTTG
  AACAGTTGGCTGTATGACTGAAGTAATAACTGATGAGATACATTTGATCTTGTAAAAATA
  GCAAAATATTATCTGAACTGGACTAGTGAAAAATCTAGTATTTGTATCCTGGCAAATAAT
  ACTAATTTATAATCCACAGTAAAGTTCATCCTTTGACTGTGCTGGAGAATTCCAGTTGTA
  TTTGAAGACTGATTTTAAAACTTTTCTGCATTTGGTAAAGGTATGTAAACTTTTCTGCAC
  TCACTGAGTAACAGCTAATCTTTATATCATATTATACTGTTATATTTTGAAAAACTGACT
  ACCTGATATAATTCCTTATTGCGATGTTTGGTATAATGACTACTTATTGTGTCCTGCTTC
  CGGTGGCCTACACCTGTTATCTCGGTGTGTTGACGTTACAGACTTCCTCGGGTAACCATA
  GGGGCGCGAACGCAAGGAGCCACAGCCGGGGTAGCTATGGCATATGATTGTGTTATGCCA
  GTAACTATCCTAGGTCGTTAACTTTTTATGCCAGCCATTTATATGCCCGGACCCCTGTTG
  CAACAATTTTTCCAACAGTCTTCCTATTACTTGTCAATAGGGGCGGGAGTACAGCGGGTC
  GGTCTGGCCGCCTATTTCCCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000745
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000745.2, NP_000736.2
• RefSeq Size: 2468 bp
• RefSeq ORF: 1407 bp
• Locus ID: 1138
• Cytogenetics: 15q25.1
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010]'
  Transcript Variant: his variant (1) represents the longer transcript and encodes the longer isoform (1).