IL1 alpha (IL1A) (BC013142) Human Untagged Clone

CAT#: SC122224

IL1A (untagged)-Human interleukin 1, alpha (cDNA clone MGC:9225 IMAGE:3875617), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL1A
• Synonyms: hematopoietin-1; IL-1A; IL1; IL1-ALPHA; IL1A (IL1F1); IL1F1; interleukin 1, alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC013142 edited
  GAAGTCAAGATGGCCAAAGTTCCAGACATGTTTGAAGACCTGAAGAACTGTTACAGTGAA
  AATGAAGAAGACAGTTCCTCCATTGATCATCTGTCTCTGAATCAGAAATCCTTCTATCAT
  GTAAGCTATGGCCCACTCCATGAAGGCTGCATGGATCAATCTGTGTCTCTGAGTATCTCT
  GAAACCTCTAAAACATCCAAGCTTACCTTCAAGGAGAGCATGGTGGTAGTAGCAACCAAC
  GGGAAGGTTCTGAAGAAGAGACGGTTGAGTTTAAGCCAATCCATCACTGATGATGACCTG
  GAGGCCATCGCCAATGACTCAGAGGAAGAAATCATCAAGCCTAGGTCAGCACCTTTTAGC
  TTCCTGAGCAATGTGAAATACAACTTTATGAGGATCATCAAATACGAATTCATCCTGAAT
  GACGCCCTCAATCAAAGTATAATTCGAGCCAATGATCAGTACCTCACGGCTGCTGCATTA
  CATAATCTGGATGAAGCAGTGAAATTTGACATGGGTGCTTATAAGTCATCAAAGGATGAT
  GCTAAAATTACCGTGATTCTAAGAATCTCAAAAACTCAATTGTATGTGACTGCCCAAGAT
  GAAGACCAACCAGTGCTGCTGAAGGAGATGCCTGAGATACCCAAAACCATCACAGGTAGT
  GAGACCAACCTCCTCTTCTTCTGGGAAACTCACGGCACTAAGAACTATTTCACATCAGTT
  GCCCATCCAAACTTGTTTATTGCCACAAAGCAAGACTACTGGGTGTGCTTGGCAGGGGGG
  CCACCCTCTATCACTGACTTTCAGATACTGGAAAACCAGGCGTAGGTCTGGAGTCTCACT
  TGTCTCACTTGTGCAGTGTTGACAGTTCATATGTACCATGTACATGAAGAAGCTAAATCC
  TTTACTGTTAGTCATTTGCTGAGCATGTACTGAGCCTTGTAATTCTAAATGAATGTTTAC
  ACTCTTTGTAAGAGTGGAACCAACACTAACATATAATGTTGTTATTTAAAGAACACCCTA
  TATTTTGCATAGTACCAATCATTTTAATTATTATTCTTCATAACAATTTTAGGAGGACCA
  GAGCTACTGACTATGGCTACCAAAAAGACTCTACCCATATTACAGATGGGCAAATTAAGG
  CATAAGAAAACTAAGAAATATGCACAATAGCAGTTGAAACAAGAAGCCACAGACCTAGGA
  TTTCATGATTTCATTTCAACTGTTTGCCTTCTACTTTTAAGTTGCTGATGAACTCTTAAT
  CAAATAGCATAAGTTTCTGGGACCTCAGTTTTATCATTTTCAAAATGGAGGGAATAATAC
  CTAAGCCTTCCTGCCGCAACAGTTTTTTATGCTAATCAGGGAGGTCATTTTGGTAAAATA
  CTTCTTGAAGCCGAGCCTCAAGATGAAGGCAAAGCACGAAATGTTATTTTTTAATTATTA
  TTTATATATGTATTTATAAATATATTTAAGATAATTATAATATACTATATTTATGGGAAC
  CCCTTCATCCTCTGAGTGTGACCAGGCATCCTCCACAATAGCAGACAGTGTTTTCTGGGA
  TAAGTAAGTTTGATTTCATTAATACAGGGCATTTTGGTCCAAGTTGTGCTTATCCCATAG
  CCAGGAAACTCTGCATTCTAGTACTTGGGAGACCTGTAATCATATAATAAATGTACATTA
  ATTACCTTGAGCCAGTAATTGGTCCGATCTTTGACTCTTTTGCCATTAAACTTACCTGGG
  CATTCTTGTTTCAATTCCACCTGCAATCAAGTCCTACAAGCTAAAATTAGATGAACTCAA
  CTTTGACAACCATGAGACCACTGTTATCAAAACTTTCTTTTCTGGAATGTAATCAATGTT
  TCTTCTAGGTTCTAAAAATTGTGATCAGACCATAATGTTACATTATTATCAACAATAGTG
  ATTGATAGAGTGTTATCAGTCATAACTAAATAAAGCTTGCAACAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC013142 unedited
  CGCGTTCGGATTTGTATACGACTCATATAGGCGGCCGCGCATTCAAATCTGGTACCGGTC
  CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGTCATTTCATTGGCGTTTGAGTCAG
  CAAAGAAGTCAAGATGGCCAAAGTTCCAGACATGTTTGAAGACCTGAAGAACTGTTACAG
  TGAAAATGAAGAAGACAGTTCCTCCATTGATCATCTGTCTCTGAATCAGAAATCCTTCTA
  TCATGTAAGCTATGGCCCACTCCATGAAGGCTGCATGGATCAATCTGTGTCTCTGAGTAT
  CTCTGAAACCTCTAAAACATCCAAGCTTACCTTCAAGGAGAGCATGGTGGTAGTAGCAAC
  CAACGGGAAGGTTCTGAAGAAGAGACGGTTGAGTTTAAGCCAATCCATCACTGATGATGA
  CCTGGAGGCCATCGCCAATGACTCAGAGGAAGAAATCATCAAGCCTAGGTCAGCACCTTT
  TAGCTTCCTGAGCAATGTGAAATACAACTTTATGAGGATCATCAAATACGAATTCATCCT
  GAATGACGCCCTCCATCAAAGTATAATTCGAGCCAATGATCAGTACCTCACGGCTGCTGC
  ATTACATAATCTGGATGAAGCAGTGAAATTTGACATGGGTGCTTATAAGTCATCAAAGGA
  TGATGCTAAAATTACCGTGATTCTAAGAATCTCAAAAACTCAATTGTATGTGACTGCCCC
  AGATGAAGACCAACCAGTGCTGCTGAAGGAGATGCCTGAGATACCCAAACCCTCACAAGT
  AGTGGAGACCAACCTTCTCTTCTTCTGGGAAACTCACGGCACTAAAAACTATTCACATCC
  GTTGCCCATCCCAAATTGGTTAATTGCCACAACGCCAAGACTACTTGGGTGGGCTTTGA
  
• Restriction Sites: Please inquire
• ACCN: BC013142
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: no
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013142.2, AAH13142.1
• RefSeq Size: 1978 bp
• Locus ID: 3552
• Cytogenetics: 2q14.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, Prion diseases, Type I diabetes mellitus
• Gene Summary: 'The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]'