Collagen II (COL2A1) (BC007252) Human Untagged Clone
CAT#: SC122236
COL2A1 (untagged)-Homo sapiens, clone MGC:15506 IMAGE:3010564, complete cds
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | COL2A1 |
Synonyms | ANFH; AOM; cartilage collagen; chondrocalcin; COL11A3; collagen, type II, alpha 1; collagen II, alpha-1 polypeptide; MGC131516; OTTHUMP00000195063; SEDC |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for BC007252 edited
CCAGGGTCCTCAAGGCCCCAGAGGTGACAAAGGAGAGGCTGGAGAGCCTGGCGAGAGAGG CCTGAAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCCCTCCTGGTCCTTC TGGAGACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGGCCC CGTCGGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCATTGGGCCTCCTGG TCCCCGTGGACGATCAGGCGAAACCGGCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCT GGTCCTCCAGGTCCCCCTGGCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCG AGAGAGAAGGGCCCCGACCCCCTGCAGTACATGCGGGCCGACCAGGCAGCCGGTGGCCTG AGACAGCATGACGCCGAGGTGGATGCCACACTCAAGTCCCTCAACAACCAGATTGAGAGC ATCCGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCAGAGACCTGAAACTC TGCCACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCACCTTG GACGCCATGAAGGTTTTCTGCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCA GCAAACGTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGG TTTGGAGAAACCATCAATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCC AACACTGCCAACGTCCAGATGACCTTCCTACGCCTGCTGTCCACGGAAGGCTCCCAGAAC ATCACCTACCACTGCAAGAACAGCATTGCCTATCTGGACGAAGCAGCTGGCAACCTCAAG AAGGCCCTGCTCATCCAGGGCTCCAATGACGTGGAGATCCGGGCAGAGGGCAATAGCAGG TTCACGTACACTGCCCTGAAGGATGGCTGCACGAAACATACCGGTAAGTGGGGCAAGACT GTTATCGAGTACCGGTCACAGAAGACCTCACGCCTCCCCATCATTGACATTGCACCCATG GACATAGGAGGGCCCGAGCAGGAATTCGGTGTGGACATAGGGCCGGTCTGCTTCTTGTAA AAACCTGAACCCAGAAACAACACAATCCGTTGCAAACCCAAAGGACCCAAGTACTTTCCA ATCTCAGTCACTCTAGGACTCTGCACTGAATGGCTGACCTGACCTGATGTCCATTCATCC CACCCTCTCACAGTTCGGACTTTTCTCCCCTCTCTTTCTAAGAGACCTGAACTGGGCAGA CTGCAAAATAAAATCTCGGTGTTCTATTTATTTATTGTCTTCCTGTAAGACCTTCGGGTC AAGGCAGAGGCAGGAAACTAACTGGTGTGAGTCAAATGCCCCCTGAGTGACTGCCCCCAG CCCAGGCCAGAAGACCTCCCTTCAGGTGCCGGGCGCAGGAACTGTGTGTGTCCTACACAA TGGTGCTATTCTGTGTCAAACACCTCTGTATTTTTTAAAACATCAATTGATATTAAAAAT GAAAAGATTATTGGAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A >OriGene 5' read for BC007252 unedited
NNTTCCCGACAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCA GGGTCCTCAAGGCCCCAGAGGTGACAAAGGAGAGGCTGGAGAGCCTGGCGAGAGAGGCCT GAAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCCCTCCTGGTCCTTCTGG AGACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGGCCCCGT CGGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCATTGGGCCTCCTGGTCC CCGTGGACGATCAGGCGAAACCGGCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCTGGT CCTCCAGGTCCCCCTGGCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCGAGA GAGAAGGGCCCCGACCCCCTGCAGTACATGCGGGCCGACCAGGCAGCCGGTGGCCTGAGA CAGCATGACGCCGAGGTGGATGCCACACTCAAGTCCCTCAACAACCAGATTGAGAGCATC CGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCAGAGACCTGAAACTCTGC CACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCACCTTGGAC GCCATGAAGGTTTTCTGCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCAGCA AACGTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGGTTT GGAGAAACCATCAATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCCCAC ACTGCCAACGTCCAGATGACCTTCCTACGCCTGCTGTCCACGGAAAGGCTCCCAGAACAT CACCTA |
Restriction Sites | Please inquire |
ACCN | BC007252 |
Insert Size | 1621 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC007252.1, AAH07252.1 |
RefSeq Size | 1621 bp |
Locus ID | 1280 |
Cytogenetics | 12q13.11 |
Protein Pathways | ECM-receptor interaction, Focal adhesion |
Gene Summary | 'This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]' |
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