Collagen II (COL2A1) (BC007252) Human Untagged Clone

CAT#: SC122236

COL2A1 (untagged)-Homo sapiens, clone MGC:15506 IMAGE:3010564, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL2A1
• Synonyms: ANFH; AOM; cartilage collagen; chondrocalcin; COL11A3; collagen, type II, alpha 1; collagen II, alpha-1 polypeptide; MGC131516; OTTHUMP00000195063; SEDC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC007252 edited
  CCAGGGTCCTCAAGGCCCCAGAGGTGACAAAGGAGAGGCTGGAGAGCCTGGCGAGAGAGG
  CCTGAAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCCCTCCTGGTCCTTC
  TGGAGACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGGCCC
  CGTCGGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCATTGGGCCTCCTGG
  TCCCCGTGGACGATCAGGCGAAACCGGCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCT
  GGTCCTCCAGGTCCCCCTGGCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCG
  AGAGAGAAGGGCCCCGACCCCCTGCAGTACATGCGGGCCGACCAGGCAGCCGGTGGCCTG
  AGACAGCATGACGCCGAGGTGGATGCCACACTCAAGTCCCTCAACAACCAGATTGAGAGC
  ATCCGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCAGAGACCTGAAACTC
  TGCCACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCACCTTG
  GACGCCATGAAGGTTTTCTGCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCA
  GCAAACGTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGG
  TTTGGAGAAACCATCAATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCC
  AACACTGCCAACGTCCAGATGACCTTCCTACGCCTGCTGTCCACGGAAGGCTCCCAGAAC
  ATCACCTACCACTGCAAGAACAGCATTGCCTATCTGGACGAAGCAGCTGGCAACCTCAAG
  AAGGCCCTGCTCATCCAGGGCTCCAATGACGTGGAGATCCGGGCAGAGGGCAATAGCAGG
  TTCACGTACACTGCCCTGAAGGATGGCTGCACGAAACATACCGGTAAGTGGGGCAAGACT
  GTTATCGAGTACCGGTCACAGAAGACCTCACGCCTCCCCATCATTGACATTGCACCCATG
  GACATAGGAGGGCCCGAGCAGGAATTCGGTGTGGACATAGGGCCGGTCTGCTTCTTGTAA
  AAACCTGAACCCAGAAACAACACAATCCGTTGCAAACCCAAAGGACCCAAGTACTTTCCA
  ATCTCAGTCACTCTAGGACTCTGCACTGAATGGCTGACCTGACCTGATGTCCATTCATCC
  CACCCTCTCACAGTTCGGACTTTTCTCCCCTCTCTTTCTAAGAGACCTGAACTGGGCAGA
  CTGCAAAATAAAATCTCGGTGTTCTATTTATTTATTGTCTTCCTGTAAGACCTTCGGGTC
  AAGGCAGAGGCAGGAAACTAACTGGTGTGAGTCAAATGCCCCCTGAGTGACTGCCCCCAG
  CCCAGGCCAGAAGACCTCCCTTCAGGTGCCGGGCGCAGGAACTGTGTGTGTCCTACACAA
  TGGTGCTATTCTGTGTCAAACACCTCTGTATTTTTTAAAACATCAATTGATATTAAAAAT
  GAAAAGATTATTGGAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  A
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC007252 unedited
  NNTTCCCGACAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCA
  GGGTCCTCAAGGCCCCAGAGGTGACAAAGGAGAGGCTGGAGAGCCTGGCGAGAGAGGCCT
  GAAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCCCTCCTGGTCCTTCTGG
  AGACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGGCCCCGT
  CGGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCATTGGGCCTCCTGGTCC
  CCGTGGACGATCAGGCGAAACCGGCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCTGGT
  CCTCCAGGTCCCCCTGGCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCGAGA
  GAGAAGGGCCCCGACCCCCTGCAGTACATGCGGGCCGACCAGGCAGCCGGTGGCCTGAGA
  CAGCATGACGCCGAGGTGGATGCCACACTCAAGTCCCTCAACAACCAGATTGAGAGCATC
  CGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCAGAGACCTGAAACTCTGC
  CACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCACCTTGGAC
  GCCATGAAGGTTTTCTGCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCAGCA
  AACGTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGGTTT
  GGAGAAACCATCAATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCCCAC
  ACTGCCAACGTCCAGATGACCTTCCTACGCCTGCTGTCCACGGAAAGGCTCCCAGAACAT
  CACCTA
  
• Restriction Sites: Please inquire
• ACCN: BC007252
• Insert Size: 1621 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC007252.1, AAH07252.1
• RefSeq Size: 1621 bp
• Locus ID: 1280
• Cytogenetics: 12q13.11
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: 'This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]'