Collagen IX (COL9A1) (BC015409) Human Untagged Clone

CAT#: SC122238

COL9A1 (untagged)-Homo sapiens, clone MGC:21955 IMAGE:4392252, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL9A1
• Synonyms: alpha-1(IX) collagen chain; alpha 1 type IX collagen; cartilage-specific short collagen; collagen, type IX, alpha 1; collagen IX, alpha-1 polypeptide; DJ149L1.1.2; EDM6; FLJ40263; MED
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC015409 edited
  CCCTCACCGGGGGCAGGAGGGACCAAGGCTGGGCCCAGAACACATAGTCCTAGGGTAACA
  GTGAAGGGGTCGTGAGGGGACAGTGACTCCCTTCCAACCCCTTCTTCATAGGGACTGTTG
  GCAAACAAAGAAAATCAACTGGGAAAATGAAGACCTGCTGGAAAATTCCAGTTTTCTTCT
  TTGTGTGCAGTTTCCTGGAACCCTGGGCATCTGCAGCTGTCAAGCGTCGCCCCAGATTCC
  CTGTCAATTCCAATTCTAATGGTGGAAATGAACTCTGTCCAAAGATCAGGATTGGCCAAG
  ATGACTTACCAGGGTTTGATCTGATCTCTCAGTTCCAGGTAGATAAAGCAGCATCTAGAA
  GAGCTATCCAGAGAGTAGTGGGATCAGCTACATTGCAGGTGGCTTACAAGTTGGGAAATA
  ATGTAGACTTCAGGATTCCAACTAGGAATTTATATCCCAGTGGACTGCCTGAAGAATACT
  CCTTCTTGACGACGTTTCGAATGACTGGAAGCACTCTCAAAAAGAACTGGAACATTTGGC
  AGATTCAGGATTCCTCTGGGAAGGAGCAAGTTGGCATAAAGATTAATGGCCAAACACAAT
  CTGTTGTATTTTCATACAAGGGACTGGATGGAAGTCTCCAAACAGCAGCCTTTTCGAATT
  TGTCCTCCTTGTTTGATTCCCAGTGGCATAAGATCATGATTGGCGTGGAGAGGAGTAGTG
  CTACTCTTTTTGTTGACTGCAACAGGATTGAATCTTTACCTATAAAGCCAAGAGGCCCAA
  TTGACATTGATGGCTTTGCTGTGCTGGGAAAACTTGCAGATAATCCTCAAGTTTCTGTTC
  CATTTGAACTTCAATGGATGCTGATCCATTGTGACCCCCTGCGGCCCAGGAGAGAAACTT
  GCCATGAGCTGCCAGCCAGAATAACGCCCAGCCAGACCACCGACGAGAGAGGTCCCCCGG
  GTGAGCAGGGTCCTCCCGGGCCTCCGGGCCCCCCTGGAGTTCCAGGCATCGATGGCATCG
  ACGGTGACCGAGGTCCTAAGGGCCCCCCGGGCCCCCCGGGTCCTGCAGGTGAACCGGGAA
  AGCCAGGAGCTCCAGGCAAGCCTGGCACACCTGGCGCTGATACCAGTCCTTGACTTTGTC
  CCTGGAATATGAAGTGAAGTAAGAAATGGCGTCTGTTCTTAGCATACCATGGTGCATCAC
  TCAAGTTACTCTTCAAGTTACCACTTTATGTGTTTTCCAAAAACTAAAGACTTTTCTAAA
  TGAAAGAAAGCACTCGAAAACCTATAATTATGATTTCCAAGAATTTTTGACTACAGTTTA
  AGACATATCCTTCATTTTGAAAGAAAAATAAATAACTGGATTTTTACTGTTAAAAAAAAA
  AAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC015409 unedited
  CCCCGATTAGGATTAGNANAACCACTTTATATAGGCGGCACGCGATTCCCGGGATATCGT
  CGACCCACGCGTCCGCCCTCACCGGGGGCAGGAGGGACCAAGGCTGGGCCCAGAACACAT
  AGTCCTAGGGTAACAGTGAAGGGGTCGTGAGGGGACAGTGACTCCCTTCCAACCCCTTCT
  TCATAGGGACTGTTGGCAAACAAAGAAAATCAACTGGGAAAATGAAGACCTGCTGGAAAA
  TTCCAGTTTTCTTCTTTGTGTGCAGTTTCCTGGAACCCTGGGCATCTGCAGCTGTCAAGC
  GTCGCCCCAGATTCCCTGTCAATTCCAATTCTAATGGTGGAAATGAACTCTGTCCAAAGA
  TCAGGATTGGCCAAGATGACTTACCAGGGTTTGATCTGATCTCTCAGTTCCAGGTAGATA
  AAGCAGCATCTAGAAGAGCTATCCAGAGAGTAGTGGGATCAGCTACATTGCAGGTGGCTT
  ACAAGTTGGGAAATAATGTAGACTTCAGGATTCCAACTAGGAATTTATATCCCAGTGGAC
  TGCCTGAAGAATACTCCTTCTTGACGACGTTTCGAATGACTGGAAGCACTCTCAAAAAGA
  ACTGGAACATTTGGCAGATTCAGGATTCCTCTGGGAAGGAGCAAGTTGGCATAAAGATTA
  ATGGCCAAACACAATCTGTTGTATTTTCATACAAGGGACTGGATGGAAGTCTCCAAACAG
  CAGCCTTTTCGAATTTGTCCTCCTTGTTTGATTCCCAGTGGCATAAGATCATGATTGGCG
  TGGAGAGGAGTAGTGCTACTCTTTTTGTTGACTGCAACAGGATTGAATCTTTACCTATNA
  AGCCAAGAGGCCCAATTGACATTGATGGGCTTGCTGTGCTGGGAAAACTTGCAGANATCC
  TCAA
  
• Restriction Sites: Please inquire
• ACCN: BC015409
• Insert Size: 1387 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015409.1, AAH15409.1
• RefSeq Size: 1387 bp
• Locus ID: 1297
• Cytogenetics: 6q13
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'