Oct4 (POU5F1) (BC020712) Human Untagged Clone

CAT#: SC122252

(untagged)-Homo sapiens, clone MGC:22487 IMAGE:4717277, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POU5F1
• Synonyms: OCT3|OCT4|OTF-3|OTF3|OTF4|Oct-3|Oct-4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC020712 edited
  CATGAGTCAGTGAACAGGGAATGGGTGAATGACATTTGTGGGTAGGTTATTTCTAGAAGT
  TAGGTGGGCAGCTTGGAAGGCAGAGGCACTTCTACAGACTATTCCTTGGGGCCACACGTA
  GGTTCTTGAATCCCGAATGGAAAGGGGAGATTGATAATTGGTGTGTTTATGTTCTTACAA
  GTCTTCTGCCTTTTAAAATCCAGTCCCAGGACATCAAAGCTCTGCAGAAAGAACTCGAGC
  AATTTGCCAAGCTCCTGAAGCAGAAGAGGATCACCCTGGGATATACACAGGCCGATGTGG
  GGCTCACCCTGGGGGTTCTATTTGGGAAGGTATTCAGCCAAACGACCATCTGCCGCTTTG
  AGGCTCTGCAGCTTAGCTTCAAGAACATGTGTAAGCTGCGGCCCTTGCTGCAGAAGTGGG
  TGGAGGAAGCTGACAACAATGAAAATCTTCAGGAGATATGCAAAGCAGAAACCCTCGTGC
  AGGCCCGAAAGAGAAAGCGAACCAGTATCGAGAACCGAGTGAGAGGCAACCTGGAGAATT
  TGTTCCTGCAGTGCCCGAAACCGACACTGCAGCAGATCAGCCACATCGCCCAGCAGCTTG
  GGCTCGAGAAGGATGTGGTCCGAGTGTGGTTCTGTAACCGGCGCCAGAAGGGCAAGCGAT
  CAAGCAGCGACTATGCACAACGAGAGGATTTTGAGGCTGCTGGGTCTCCTTTCTCAGGGG
  GACCAGTGTCCTTTCCTCTGGCCCCAGGGCCCCATTTTGGTACCCCAGGCTATGGGAGCC
  CTCACTTCACTGCACTGTACTCCTCGGTCCCTTTCCCTGAGGGGGAAGCCTTTCCCCCTG
  TCTCCGTCACCACTCTGGGCTCTCCCATGCATTCAAACTGAGGTGCCTGCCCTTCTAGGA
  ATGGGGGACAGGGGGAGGGGAGGAGCTAGGGAAAGAAAACCTGGAGTTTGTGCCAGGGTT
  TTTGGGATTAAGTTCTTCATTCACTAAGGAAGGAATTGGGAACACAAAGGGTGGGGGCAG
  GGGAGTTTGGGGCAACTGGTTGGAGGGAAGGTGAAGTTCAATGATGCTCTTGATTTTAAT
  CCCACATCATGTATCACTTTTTTCTTAAATAAAGAAGCCTGGGACACAGTAGATAGGCAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC020712 unedited
  TTCGGATTTGTATACGACNACTATAGGGCGGCCGCGAATTCGCCATTACGGCCGGGGCAT
  GAGTCAGTGAACAGGGAATGGGTGAATGACATTTGTGGGTAGGTTATTTCTAGAAGTTAG
  GTGGGCAGCTTGGAAGGCAGAGGCACTTCTACAGACTATTCCTTGGGGCCACACGTAGGT
  TCTTGAATCCCGAATGGAAAGGGGAGATTGATAATTGGTGTGTTTATGTTCTTACAAGTC
  TTCTGCCTTTTAAAATCCAGTCCCAGGACATCAAAGCTCTGCAGAAAGAACTCGAGCAAT
  TTGCCAAGCTCCTGAAGCAGAAGAGGATCACCCTGGGATATACACAGGCCGATGTGGGGC
  TCACCCTGGGGGTTCTATTTGGGAAGGTATTCAGCCAAACGACCATCTGCCGCTTTGAGG
  CTCTGCAGCTTAACTTCAAGAACATGTGTAAGCTGCGGCCCTTGCTGCAGAAGTGGGTGG
  AGGAAGCTGACAACAATGAAAATCTTCAGGAGATATGCAAAGCAGATACCCTCGTGCANG
  CCCGAAAGAGAAAGCGAACCAGTATCGAGAACCGAGTGAGAGGCAACCTGGAGAATTTGT
  TCCTGCAGTGCCCGANACCGACACTGCAGCAGATCAGCCACATCGCCCAGCAGCTTGGGC
  TCGAGAAGGATGTGGTCCGAGTGTGGTTCTGTAACCGGCGCCAGAAGGGCAAGCGATCAA
  GCAGCGACTATGCACAACGAGAGGATTTTGAGGCTGCTGGGTCTCCTTTCTCAGGGGGAC
  AGTGTTCCTTCTCTGGCCCAGGGCCCCATTTTTGTACCCCAGCTAGGGAAGCCTCACTGA
  CTGCACTGTACTCCTTGGTCCCTTTCCCTGAGGGGAAACCTTTCCCCCTGTGTCCGGACC
  AATCTGGGTTCTCCTGCATCAAACTGAGGGCCG
  
• Restriction Sites: Please inquire
• ACCN: BC020712
• Insert Size: 1167 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020712.1
• RefSeq Size: 1167 bp
• Locus ID: 5460
• Cytogenetics: 6p21.33
• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: 'This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]'