CAD (BC014178) Human Untagged Clone

CAT#: SC122272

CAD (untagged)-Homo sapiens, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase, clone MGC:20745 IMAGE:4580779, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAD
• Synonyms: CDG1Z; EIEE50; GATD4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC014178 edited
  GGCACGAGGCCCACCACAGCGGAGCATCCTGGAGCAGCTAGCTGAGAAAAACTTTGAGCT
  GGTGATTAACCTGTCAATGCGTGGAGCTGGGGGCCGGCGTCTCTCTTCCTTTGTCACCAA
  GGGCTACCGCACCCGACGCTTGGCCGCTGACTTCTCCGTGCCCCTAATCATCGATATCAA
  GTGCACCAAACTCTTTGTGGAGGCCCTAGGCCAGATCGGGCCAGCCCCTCCTTTGAAGGT
  GCATGTTGACTGTATGACCTCCCAAAAGCTTGTGCGACTGCCGGGATTGATTGATGTCCA
  TGTGCACCTGCGGGAACCAGGTGGGACACATAAGGAGGACTTTGCTTCAGGCACAGCCGC
  TGCCCTGGCTGGGGGTATCACCATGGTGTGTGCCATGCCTAATACCCGGCCCCCCATCAT
  TGACGCCCCTGCTCTGGCCCTGGCCCAGAAGCTGGCAGAGGCTGGCGCCCGGTGCGACTT
  TGCGCTATTCCTTGGGGCCTCGTCTGAAAATGCAGGAACCTTGGGCACCGTGGCCGGGTC
  TGCAGCCGGGCTGAAGCTTTACCTCAATGAGACCTTCTCTGAGCTGCGGCTGGACAGCGT
  GGTCCAGTGGATGGAGCATTTCGAGACATGGCCCTCCCACCTCCCCATTGTGGCTCACGC
  GGAGCAGCAAACCGTGGCTGCTGTCCTCATGGTGGCTCAGCTCACTCAGCGCTCAGTGCA
  CATATGTCACGTGGCACGGAAGGAGGAGATCCTGCTAATTAAAGCTGCAAAGGCACGGGG
  CTTGCCAGTGACCTGCGAGGTGGCTCCCCACCACCTGTTCCTAAGCCATGATGACCTGGA
  GCGCCTGGGGCCTGGGAAGGGGGAGGTCCGGCCTGAGCTTGGCTCCCGCCAGGATGTGGA
  AGCCCTGTGGGAGAACATGGCTGTCATCGACTGCTTTGCCTCAGACCATGCTCCCCATAC
  CTTGGAGGAGAAGTGTGGGTCCAGGCCCCCACCTGGGTTCCCAGGGTTAGAGACCATGCT
  GCCACTACTCCTGACGGCTGTAAGCGAGGGCCGGCTCAGCCTGGACGACCTGCTGCAGCG
  ATTGCACCACAATCCTCGGCGCATCTTTCACCTGCCTCCGCAGGAGGACACCTATGTGGA
  GGTGGATCTGGAGCATGAGTGGACAATTCCCAGCCACATGCCCTTCTCCAAGGCCCACTG
  GACACCTTTTGAAGGGCAGAAAGTGAAGGGCACCGTCCGCCGTGTGGTCCTGCGAGGGGA
  GGTTGCCTATATCGATGGGCAGGTTCTGGTACCCCCGGGCTATGGACAGGATGTACGGAA
  GTGGCCACAGGGGGCTGTTCCTCAGCTCCCACCCTCAGCCCCTGCCACTAGTGAGATGAC
  CACGACACCTGAAAGACCCCGCCGTGGCATCCCAGGGCTTCCTGATGGCCGCTTCCATCT
  GCCGCCCCGAATCCATCGAGCCTCCGACCCAGGTTTGCCAGCTGAGGAGCCAAAGGAGAA
  GTCCTCTCGGAAGGTAGCCGAGCCAGAGCTGATGGGAACCCCTGATGGCACCTGCTACCC
  TCCACCACCAGTACCGAGACAGGCATCTCCCCAGAACCTGGGGACCCCTGGCTTGCTGCA
  CCCCCAGACCTCACCCCTGCTGCACTCATTAGTGGGCCAACATATCCTGTCCGTCCAGCA
  GTTCACCAAGGATCAGATGTCTCACCTGTTCAATGTGGCACACACACTGCGTATGATGGT
  GCAGAAGGAGCGGAGCCTCGACATCCTGAAGGGGAAGGTCATGGCCTCCATGTTCTATGA
  AGTGAGCACACGGACCAGCAGCTCCTTTGCAGCAGCCATGGCCCGGCTGGGAGGTGCTGT
  GCTCAGCTTCTCGGAAGCCACATCGTCCGTCCAGAAGGGCGAATCCCTGGCTGACTCCGT
  GCAGACCATGAGCTGCTATGCCGACGTCGTCGTGCTCCGGCACCCCCAGCCTGGAGCAGT
  GGAGCTGGCCGCCAAGCACTGCCGGAGGCCAGTGATCAATGCTGGGGATGGGGTCGGAGA
  GCACCCCACCCAGGCCCTGCTGGACATCTTCACCATCCGTGAGGAGCTGGGAACTGTCAA
  TGGCATGACGATCACGATGGTGGGTGACCTGAAGCACGGACGCACAGTACATTCCCTGGC
  CTGCCTGCTCACCCAGTATCGTGTCAGCCTGCGCTACGTGGCACCTCCCAGCCTGCGCAT
  GCCACCCACTGTGCGGGCCTTCGTGGCCTCCCGCGGCACCAAGCAGGAGGAATTCGAGAG
  CATTGAGGAGGCGCTGCCTGACACTGATGTGCTCTACATGACTCGAATCCAGAAGGAACG
  ATTTGGCTCTACCCAGGAGTACGAAGCTTGCTTTGGTCAGTTCATCCTCACTCCCCACAT
  CATGACCCGGGCCAAGAAGAAGATGGTGGTGATGCACCCGATGCCCCGTGTCAACGAGAT
  AAGCGTGGAAGTGGACTCGGATCCCCGCGCAGCCTACTTCCGCCAGGCTGAGAACGGCAT
  GTACATCCGCATGGCTCTGTTAGCCACCGTGCTGGGCCGTTTCTAGGGCCTGGCTTCCTC
  AGCCTCTTCTCTTTAGGCCCAGCTGCTGGGCAAGGAATTCCAGTGCCTCCTACGGGGGCA
  GCACACTTAGATATTCCTGGACATCCAGATAGCTCACATGTGCTGACCACACTTCAGGCT
  CTGGACTGGAGCTCTCTGGCATGGGGGTGGGGCCTCAGATGCTGGGGCCCAGTCTGCCCC
  ATCTTCATTCCTGCACCTTAAACCTGTACAGTCATTTTTCTACTGACTTAATAAACAGCC
  GAGCTGTCCCTTGAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC014178 unedited
  NGGGATTTATTTGTATACCCTCATATAGGCGGCCGCAGAATTCGCACGAGGGCCCACCAC
  AGCGGAGCATCCTGNGACAGCTAGCTGAGAAAAACTTTGAGCTGGTGATTAACCTGTCAA
  TGCGTGGAGCTGGGGGCCGGCGTCTCTCTTCCTTTGTCACCAAGGGCTACCGCACCCGAC
  GCTTGGCCGCTGACTTCTCCGTGCCCCTAATCATCGATATCAAGTGCACCAAACTCTTTG
  TGGAGGCCCTAGGCCAGATCGGGCCAGCCCCTCCTTTGAAGGTGCATGTTGACTGTATGA
  CCTCCCAAAAGCTTGTGCGACTGCCGGGATTGATTGATGTCCATGTGCACCTGCGGGAAC
  CAGGTGGGACACATAAGGAGGACTTTGCTTCAGGCACAGCCGCTGCCCTGGCTGGGGGTA
  TCACCATGGTGTGTGCCATGCCTAATACCCGGCCCCCCATCATTGACGCCCCTGCTCTGG
  CCCTGGCCCAGAAGCTGGCAGAGGCTGGCGCCCGGTGCGACTTTGCGCTATTCCTTGNGG
  CCTCGTCTGAAAATGCAGGAACCTTGGGCACCGTGGCCGGGTCTGCAGCCGGGCTGAAGC
  TTTACCTCAATGAGACCTTCTCTGAGCTGCGGCTGGACAGCGTGGTCCAGTGGATGGAGC
  ATTTCGAGACATGGCCCTCCCACCTCCCCATTGTGGCTCACGCGGAGCAGCAAACCGTGG
  CTGCTGTCCTCATGGTGGCTCAGCTCACTCAGCGCTCAGTGCACATATGTCACGTGGCAC
  GGAAGGAGGAGATCCTGCTAATTAAAGCTGCAAAGGCACGGGGCTTGCCAGTGACCTGCA
  AGGTGGCTCCCAACCACCTNGTTCTAAACCATGATGACCTGAAGCGCTGGGGCCTGGGAA
  
• Restriction Sites: Please inquire
• ACCN: BC014178
• Insert Size: 2851 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014178.1, AAH14178.1
• RefSeq Size: 2851 bp
• Locus ID: 790
• Cytogenetics: 2p23.3
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Pyrimidine metabolism
• Gene Summary: 'The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]'