HNF1 beta (HNF1B) (NM_000458) Human Untagged Clone

CAT#: SC122562

HNF1B (untagged)-Human HNF1 homeobox B (HNF1B), transcript variant 1


  "NM_000458" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HNF1B
Synonyms FJHN; HNF-1-beta; HNF-1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; TCF-2; TCF2; VHNF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000458 edited
ATCATGGCAAGTTAGAAGTTTTCTGACTCCTTTCGGAGGAGCCTCCGGGACCCCGGGGAG
TAACAGGTGTCTGGAGGCTGAAGGGTGGAGGGGTTCCTGGATTTGGGGTTTGCTTGTGAA
ACTCCCCTCCACCCTCCTCTCTCGCACCCACCCACCCCCTCACCCCCTTCTTTTTCCGTC
CTTGGAAAATGGTGTCCAAGCTCACGTCGCTCCAGCAAGAACTCCTGAGCGCCCTGCTGA
GCTCCGGGGTCACCAAGGAGGTGCTGGTTCAGGCCTTGGAGGAGTTGCTGCCATCCCCGA
ACTTCGGGGTGAAGCTGGAGACGCTGCCCCTGTCCCCTGGCAGCGGGGCCGAGCCCGACA
CCAAGCCGGTCTTCCATACTCTCACCAACGGCCACGCCAAGGGCCGCTTGTCCGGCGACG
AGGGCTCCGAGGACGGCGACGACTATGACACACCTCCCATCCTCAAGGAGCTGCAGGCGC
TCAACACCGAGGAGGCGGCGGAGCAGCGGGCGGAGGTGGACCGGATGCTCAGTGAGGACC
CTTGGAGGGCTGCTAAAATGATCAAGGGTTACATGCAGCAACACAACATCCCCCAGAGGG
AGGTGGTCGATGTCACCGGCCTGAACCAGTCGCACCTCTCCCAGCATCTCAACAAGGGCA
CCCCTATGAAGACCCAGAAGCGTGCCGCTCTGTACACCTGGTACGTCAGAAAGCAACGAG
AGATCCTCCGACAATTCAACCAGACAGTCCAGAGTTCTGGAAATATGACAGACAAAAGCA
GTCAGGATCAGCTGCTGTTTCTCTTTCCAGAGTTCAGTCAACAGAGCCATGGGCCTGGGC
AGTCCGATGATGCCTGCTCTGAGCCCACCAACAAGAAGATGCGCCGCAACCGGTTCAAAT
GGGGGCCCGCGTCCCAGCAAATCTTGTACCAGGCCTACGATCGGCAAAAGAACCCCAGCA
AGGAAGAGAGAGAGGCCTTAGTGGAGGAATGCAACAGGGCAGAATGTTTGCAGCGAGGGG
TGTCCCCCTCCAAAGCCCACGGCCTGGGCTCCAACTTGGTCACTGAGGTCCGTGTCTACA
ACTGGTTTGCAAACCGCAGGAAGGAGGAGGCATTCCGGCAAAAGCTGGCCATGGACGCCT
ATAGCTCCAACCAGACTCACAGCCTGAACCCTCTGCTCTCCCACGGCTCCCCCCACCACC
AGCCCAGCTCCTCTCCTCCAAACAAGCTGTCAGGAGTGCGCTACAGCCAGCAGGGAAACA
ATGAGATCACTTCCTCCTCAACAATCAGTCACCATGGCAACAGCGCCATGGTGACCAGCC
AGTCGGTTTTACAGCAAGTCTCCCCAGCCAGCCTGGACCCAGGCCACAATCTCCTCTCAC
CTGATGGTAAAATGATCTCAGTCTCAGGAGGAGGTTTGCCCCCAGTCAGCACCTTGACGA
ATATCCACAGCCTCTCCCACCATAATCCCCAGCAATCTCAAAACCTCATCATGACACCCC
TCTCTGGAGTCATGGCAATTGCACAAAGCCTCAACACCTCCCAAGCACAGAGTGTCCCTG
TCATCAACAGTGTGGCCGGCAGCCTGGCAGCCCTGCAGCCCGTCCAGTTCTCCCAGCAGC
TGCACAGCCCTCACCAGCAGCCCCTCATGCAGCAGAGCCCAGGCAGCCACATGGCCCAGC
AGCCCTTCATGGCAGCTGTGACTCAGCTGCAGAACTCACACATGTACGCACACAAGCAGG
AACCCCCCCAGTATTCCCACACCTCCCGGTTTCCATCTGCAATGGTGGTCACAGATACCA
GCAGCATCAGTACACTCACCAACATGTCTTCAAGTAAACAGTGTCCTCTACAAGCCTGGT
GATGCCCACACACCACTTACTTCGTGCGCAACAACAAGGACCCTGTTTTCCACACCATCA
CCCTCTGGGCAGCTGTCATGGAAAAGCCCAGTGACCTGACCAGCACCTGCGAGAGGTCCC
TGCTTACCTGACGGACGTCCTGCTGGCACCTCAGACAATCCACTCTCAGGAGGCGCAGCC
CGAAGCCCAGTTTCCCTTCTATGCAGTATTGCCACAATGCCTCTCCCACGATGTCAAGGA
CTCCTGTCTGTCCTGGAGGTGGGAGACAAGGAACCTCCGAAGAGGAAGCAAGAAAGCCGT
ACTGTCTATGTTGTGATCCTTCATCGAACAAACTGATGCGAAAACTTGAATCTGTTACTG
AAATGAGGAGAGAAGGACATGTGCTATTGAACTGAGCCAAACACACTGTAAATATCCACA
GACTCCCTCCCCTGCCCCCATCCCAAATGATCTTGAGATTTCTTTTAAAGAAGTAAATTT
GTCCAATGGCTGTAAACTATAAACTACTGTAATTAAGTGCAATTTCCCCTCTGTGTCCTC
TCCCCTCTGCCCTGTATATAATACTAAAGTGTCTATTAGTTTTCTTTGTAAAGGTCAGAG
TCAAAATTTCAAAAGTGATCTGTCCCCTCTCCCCTCATGGAGAAACATCCTAAGTGGGAA
GTGAAGCCCCTTGTCCTCTCCCGCGGGCCTGGACACTTATGGGGACAGCATACCTTGGAC
TGACTACCAGCTAACTCCAGTCTCCTGACATTAAGACACACCTCTGGATCCCTGGAGGGG
CTGAATGTAGTGTGTCAGAGTAACATGCCAGCTTCCTGTGGGCCAGGAGCTCAGCCGTGC
ACTCCCTAAGAAACCCCAGGGCAGGGAAACTGGCTGTTTGATAGCAGAAGAAAAAGTTGC
AGTCTCAGAAAGCCTTCCATTAAAACAATTTATTTTATCACTAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_000458 unedited
GCGGGCCGCGNAATTCCCGGGGTATCGTCGACCCACGCGTCCGATCATGGCAAGTTAGAA
GTTTTCTGACTCCTTTCGGAGGAGCCTCCGGGACCCCGGGGAGTAACAGGTGTCTGGAGG
CTGAAGGGTGGAGGGGTTCCTGGATTTGGGGTTTGCTTGTGAAACTCCCCTCCACCCTCC
TCTCTCGCACCCACCCACCCCCTCACCCCCTTCTTTTTCCGTCCTTGGAAAATGGTGTCC
AAGCTCACGTCGCTCCAGCAAGAACTCCTGAGCGCCCTGCTGAGCTCCGGGGTCACCAAG
GAGGTGCTGGTTCAAGCCTTGGAGGAGTTGCTGCCATCCCCGAACTTCGGGGTGAAGCTG
GAGACGCTGCCCCTGTCCCCTGGCAGCGGGGCCGAGCCCGACACCAAGCCGGTCTTCCAT
ACTCTCACCAACGGCCACGCCAAGGGCCGCTTGTCCGGCGACGAGGGCTCCGAGGACGGC
GACGACTATGACACACCTCCCATCCTCAAGGAGCTGCAGGCGCTCAACACCGAGGAGGCG
GCGGATCATCGGGCGGAGGTGGACCGGATGCTCAGTGAGGACCCTTGGAGGGCTGCTAAA
ATGATCAAGGGTTACATGCAGGCACACAACATCCCCCAGAGGGAGGTGGTCGATGTCACC
GGCCTGAACCAGTCGCACCTCTCCCAGCATCTCAACCAGGGCACCCCTATGAAGACCCAT
GATCGTGCCGCTCTGTACACCTGGTACGTCAGAAAGCAACGAGAGATCCTCCGACAATCA
ACCAGACAGTCCAGAGTTCTGGAAATATGACAGACANAAGCAGTCAGGATCAGCTGCTGT
TTCTCTTTCCGAGTTCAGTCACAGAGC
Restriction Sites Please inquire     
ACCN NM_000458
Insert Size 2818 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000458.1, NP_000449.1
RefSeq Size 2816 bp
RefSeq ORF 1674 bp
Locus ID 6928
Cytogenetics 17q12
Protein Families Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways Maturity onset diabetes of the young
Gene Summary 'This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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