HNF1 beta (HNF1B) (NM_000458) Human Untagged Clone

CAT#: SC122562

HNF1B (untagged)-Human HNF1 homeobox B (HNF1B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HNF1B
• Synonyms: FJHN; HNF-1-beta; HNF-1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; TCF-2; TCF2; VHNF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000458 edited
  ATCATGGCAAGTTAGAAGTTTTCTGACTCCTTTCGGAGGAGCCTCCGGGACCCCGGGGAG
  TAACAGGTGTCTGGAGGCTGAAGGGTGGAGGGGTTCCTGGATTTGGGGTTTGCTTGTGAA
  ACTCCCCTCCACCCTCCTCTCTCGCACCCACCCACCCCCTCACCCCCTTCTTTTTCCGTC
  CTTGGAAAATGGTGTCCAAGCTCACGTCGCTCCAGCAAGAACTCCTGAGCGCCCTGCTGA
  GCTCCGGGGTCACCAAGGAGGTGCTGGTTCAGGCCTTGGAGGAGTTGCTGCCATCCCCGA
  ACTTCGGGGTGAAGCTGGAGACGCTGCCCCTGTCCCCTGGCAGCGGGGCCGAGCCCGACA
  CCAAGCCGGTCTTCCATACTCTCACCAACGGCCACGCCAAGGGCCGCTTGTCCGGCGACG
  AGGGCTCCGAGGACGGCGACGACTATGACACACCTCCCATCCTCAAGGAGCTGCAGGCGC
  TCAACACCGAGGAGGCGGCGGAGCAGCGGGCGGAGGTGGACCGGATGCTCAGTGAGGACC
  CTTGGAGGGCTGCTAAAATGATCAAGGGTTACATGCAGCAACACAACATCCCCCAGAGGG
  AGGTGGTCGATGTCACCGGCCTGAACCAGTCGCACCTCTCCCAGCATCTCAACAAGGGCA
  CCCCTATGAAGACCCAGAAGCGTGCCGCTCTGTACACCTGGTACGTCAGAAAGCAACGAG
  AGATCCTCCGACAATTCAACCAGACAGTCCAGAGTTCTGGAAATATGACAGACAAAAGCA
  GTCAGGATCAGCTGCTGTTTCTCTTTCCAGAGTTCAGTCAACAGAGCCATGGGCCTGGGC
  AGTCCGATGATGCCTGCTCTGAGCCCACCAACAAGAAGATGCGCCGCAACCGGTTCAAAT
  GGGGGCCCGCGTCCCAGCAAATCTTGTACCAGGCCTACGATCGGCAAAAGAACCCCAGCA
  AGGAAGAGAGAGAGGCCTTAGTGGAGGAATGCAACAGGGCAGAATGTTTGCAGCGAGGGG
  TGTCCCCCTCCAAAGCCCACGGCCTGGGCTCCAACTTGGTCACTGAGGTCCGTGTCTACA
  ACTGGTTTGCAAACCGCAGGAAGGAGGAGGCATTCCGGCAAAAGCTGGCCATGGACGCCT
  ATAGCTCCAACCAGACTCACAGCCTGAACCCTCTGCTCTCCCACGGCTCCCCCCACCACC
  AGCCCAGCTCCTCTCCTCCAAACAAGCTGTCAGGAGTGCGCTACAGCCAGCAGGGAAACA
  ATGAGATCACTTCCTCCTCAACAATCAGTCACCATGGCAACAGCGCCATGGTGACCAGCC
  AGTCGGTTTTACAGCAAGTCTCCCCAGCCAGCCTGGACCCAGGCCACAATCTCCTCTCAC
  CTGATGGTAAAATGATCTCAGTCTCAGGAGGAGGTTTGCCCCCAGTCAGCACCTTGACGA
  ATATCCACAGCCTCTCCCACCATAATCCCCAGCAATCTCAAAACCTCATCATGACACCCC
  TCTCTGGAGTCATGGCAATTGCACAAAGCCTCAACACCTCCCAAGCACAGAGTGTCCCTG
  TCATCAACAGTGTGGCCGGCAGCCTGGCAGCCCTGCAGCCCGTCCAGTTCTCCCAGCAGC
  TGCACAGCCCTCACCAGCAGCCCCTCATGCAGCAGAGCCCAGGCAGCCACATGGCCCAGC
  AGCCCTTCATGGCAGCTGTGACTCAGCTGCAGAACTCACACATGTACGCACACAAGCAGG
  AACCCCCCCAGTATTCCCACACCTCCCGGTTTCCATCTGCAATGGTGGTCACAGATACCA
  GCAGCATCAGTACACTCACCAACATGTCTTCAAGTAAACAGTGTCCTCTACAAGCCTGGT
  GATGCCCACACACCACTTACTTCGTGCGCAACAACAAGGACCCTGTTTTCCACACCATCA
  CCCTCTGGGCAGCTGTCATGGAAAAGCCCAGTGACCTGACCAGCACCTGCGAGAGGTCCC
  TGCTTACCTGACGGACGTCCTGCTGGCACCTCAGACAATCCACTCTCAGGAGGCGCAGCC
  CGAAGCCCAGTTTCCCTTCTATGCAGTATTGCCACAATGCCTCTCCCACGATGTCAAGGA
  CTCCTGTCTGTCCTGGAGGTGGGAGACAAGGAACCTCCGAAGAGGAAGCAAGAAAGCCGT
  ACTGTCTATGTTGTGATCCTTCATCGAACAAACTGATGCGAAAACTTGAATCTGTTACTG
  AAATGAGGAGAGAAGGACATGTGCTATTGAACTGAGCCAAACACACTGTAAATATCCACA
  GACTCCCTCCCCTGCCCCCATCCCAAATGATCTTGAGATTTCTTTTAAAGAAGTAAATTT
  GTCCAATGGCTGTAAACTATAAACTACTGTAATTAAGTGCAATTTCCCCTCTGTGTCCTC
  TCCCCTCTGCCCTGTATATAATACTAAAGTGTCTATTAGTTTTCTTTGTAAAGGTCAGAG
  TCAAAATTTCAAAAGTGATCTGTCCCCTCTCCCCTCATGGAGAAACATCCTAAGTGGGAA
  GTGAAGCCCCTTGTCCTCTCCCGCGGGCCTGGACACTTATGGGGACAGCATACCTTGGAC
  TGACTACCAGCTAACTCCAGTCTCCTGACATTAAGACACACCTCTGGATCCCTGGAGGGG
  CTGAATGTAGTGTGTCAGAGTAACATGCCAGCTTCCTGTGGGCCAGGAGCTCAGCCGTGC
  ACTCCCTAAGAAACCCCAGGGCAGGGAAACTGGCTGTTTGATAGCAGAAGAAAAAGTTGC
  AGTCTCAGAAAGCCTTCCATTAAAACAATTTATTTTATCACTAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000458 unedited
  GCGGGCCGCGNAATTCCCGGGGTATCGTCGACCCACGCGTCCGATCATGGCAAGTTAGAA
  GTTTTCTGACTCCTTTCGGAGGAGCCTCCGGGACCCCGGGGAGTAACAGGTGTCTGGAGG
  CTGAAGGGTGGAGGGGTTCCTGGATTTGGGGTTTGCTTGTGAAACTCCCCTCCACCCTCC
  TCTCTCGCACCCACCCACCCCCTCACCCCCTTCTTTTTCCGTCCTTGGAAAATGGTGTCC
  AAGCTCACGTCGCTCCAGCAAGAACTCCTGAGCGCCCTGCTGAGCTCCGGGGTCACCAAG
  GAGGTGCTGGTTCAAGCCTTGGAGGAGTTGCTGCCATCCCCGAACTTCGGGGTGAAGCTG
  GAGACGCTGCCCCTGTCCCCTGGCAGCGGGGCCGAGCCCGACACCAAGCCGGTCTTCCAT
  ACTCTCACCAACGGCCACGCCAAGGGCCGCTTGTCCGGCGACGAGGGCTCCGAGGACGGC
  GACGACTATGACACACCTCCCATCCTCAAGGAGCTGCAGGCGCTCAACACCGAGGAGGCG
  GCGGATCATCGGGCGGAGGTGGACCGGATGCTCAGTGAGGACCCTTGGAGGGCTGCTAAA
  ATGATCAAGGGTTACATGCAGGCACACAACATCCCCCAGAGGGAGGTGGTCGATGTCACC
  GGCCTGAACCAGTCGCACCTCTCCCAGCATCTCAACCAGGGCACCCCTATGAAGACCCAT
  GATCGTGCCGCTCTGTACACCTGGTACGTCAGAAAGCAACGAGAGATCCTCCGACAATCA
  ACCAGACAGTCCAGAGTTCTGGAAATATGACAGACANAAGCAGTCAGGATCAGCTGCTGT
  TTCTCTTTCCGAGTTCAGTCACAGAGC
  
• Restriction Sites: Please inquire
• ACCN: NM_000458
• Insert Size: 2818 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000458.1, NP_000449.1
• RefSeq Size: 2816 bp
• RefSeq ORF: 1674 bp
• Locus ID: 6928
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: 'This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).