Myelin Protein Zero (MPZ) (NM_000530) Human Untagged Clone

CAT#: SC122563

MPZ (untagged)-Human myelin protein zero (MPZ)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MPZ
• Synonyms: CHM; CHN2; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; DSS; HMSNIB; MPP; P0
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000530 edited
  CACTTTCTCAACCCCACAGATGCTCCGGGCCCCTGCCCCTGCCCCAGCTATGGCTCCTGG
  GGCTCCCTCATCCAGCCCCAGCCCTATCCTGGCTGTGCTGCTCTTCTCTTCTTTGGTGCT
  GTCCCCGGCCCAGGCCATCGTGGTTTACACCGACAGGGAGGTCCATGGTGCTGTGGGCTC
  CCGGGTGACCCTGCACTGCTCCTTCTGGTCCAGTGAGTGGGTCTCAGATGACATCTCCTT
  CACCTGGCGCTACCAGCCCGAAGGGGGCAGAGATGCCATTTCGATCTTCCACTATGCCAA
  GGGACAACCCTACATTGACGAGGTGGGGACCTTCAAAGAGCGCATCCAGTGGGTAGGGGA
  CCCTCGCTGGAAGGATGGCTCCATTGTCATACACAACCTAGACTACAGTGACAATGGCAC
  GTTCACTTGTGACGTCAAAAACCCTCCAGACATAGTGGGCAAGACCTCTCAGGTCACGCT
  GTATGTCTTTGAAAAAGTGCCAACTAGGTACGGGGTCGTTCTGGGAGCTGTGATCGGGGG
  TGTCCTCGGGGTGGTGCTGTTGCTGCTGCTGCTTTTCTACGTGGTTCGGTACTGCTGGCT
  ACGCAGGCAGGCGGCCCTGCAGAGGAGGCTCAGTGCTATGGAGAAGGGGAAATTGCACAA
  GCCAGGAAAGGACGCGTCGAAGCGCGGGCGGCAGACGCCAGTGCTGTATGCAATGCTGGA
  CCACAGCAGAAGCACCAAAGCTGTCAGTGAGAAGAAGGCCAAGGGGCTGGGGGAGTCTCG
  CAAGGATAAGAAATAGCGGTTAGCGGGCCGGGCGGGGGATCGGGGGTTAGGGGTGGAGTC
  CGCCAAAGGCCCAAAGGTGATGGTCATCGAGATGGAGCTACGAAAGGATGAGCAGAGCCC
  GGAGCTCCGGCCTGCTGTCAAGTCCCCCAGCAGAACCAGCCTCAAAAACGCCCTCAAGAA
  CATGATGGGCCTGAACTCGGACAAGTGATCGCCACCCCCCCACCCCAGGCCCTGCCAGAG
  CAGGGGGACCTAGGCTCCTCTTACCCCCGTCTAGGTGCTTTCCCTCTTTGCTCCCCCGCC
  CTGCCCTGCCCTCACCTCCCTTTGAGATGTAAGTTTCATTCCAGAATTCATTCCCCAGCT
  CCATACTCTGTCCCCCCAGCTAATACCCAGAGCACCCAGATCAGACTCTCCTTCAGGGTT
  TATTTAGGTTATTATTTTTTATTTTTTAATCCATTCTTTGTTTGTTTACCTGTGCTCATC
  CTCTGCCCTTACACCCATGACTGAGGACCAATGACGTCATGTGGCTTTTGCAATTCACGC
  CCCCCTTAAGTCCTTAATGAAGAGCCAGCCCAAGTAGAGGGGCCCCTGATCCTCACACTT
  CAGTATAGCATTGGTTCCCCCTGACCACTTTGGAGCACTGTTCTGGGACTCCAGGTCTTG
  AGGAGAGAGACAGAGAGAGAGAATGGATCCTCATAGGTCAGGGAGTGGGGGAGGGGGCAA
  ATGAGCCTTAAGAAATGGTTTTTAAACAACCAAACAAAAAGCAGGAAAAACAAATGGGAA
  ATGGGGGGGCGGGGGGGAGGAAGAGGCTGCACTGCAGCCACAGGGGATTCTTAGGATTTT
  TCTACATTCTGTATATTTCTTCTCAAACCTCCAAATGTCCTTAAATGTTTAATAAACACT
  GACATTTCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000530 unedited
  NNAAGAGTTCAAAATTTGTAATACGACTCACTATAGGGACCGGCCGCGAATTCGCACGAG
  GCACTTTCTCAACCCCACANAGCTCCGGGCCCCTGCCCCTGCCCCAGCTATGGCTCCTGG
  GGCTCCCTCATCCAGCCCCAGCCCTATCCTGGCTGTGCTGCTCTTCTCTTCTTTGGTGCT
  GTCCCCGGCCCAGGCCATCGTGGTTTACACCGACAGGGAGGTCCATGGTGCTGTGGGCTC
  CCGGGTGACCCTGCACTGCTCCTTCTGGTCCAGTGAGTGGGTCTCAGATGACATCTCCTT
  CACCTGGCGCTACCAGCCCGAAGGGGGCAGAGATGCCATTTCGATCTTCCACTATGCCAA
  GGGACAACCCTACATTGACGAGGTGGGGACCTTCAAAGAGCGCATCCAGTGGGTAGGGGA
  CCCTCGCTGGAAGGATGGCTCCATTGTCATACACAACCTAGACTACAGTGACAATGGCAC
  GTTCACTTGTGACGTCAAAAACCCTCCAGACATAGTGGGCAAGACCTCTCAGGTCACGCT
  GTATGTCTTTGAAAAAGTGCCAACTAGGTACGGGGTCGTTCTGGGAGCTGTGATCGGGGG
  TGTCCTCGGGGTGGTGCTGTTGCTGCTGCTGCTTTTCTACGTGGTTCGGTACTGCTGGCT
  ACGCAGGCAGGCGGCCCTGCAGAGGAGGCTCAGTGCTATGGAGAAGGGGAAATTGCACAA
  GCCAGGANAGGACGCGTCGAAGCGCGGGCGGCAGACGCCAGTGCTGTATGCAATGCTGGA
  CCACAGCAGAAGCACCAAAGCTGTCAGTGAGAAGAAGGCCAAGGGNGCTGGGGGAGTCTC
  GCAAGGATAAGAAATAGCGTTAGCGGGGCCGGGCGGGGATCGGGGGTTAGGGGTGGGAGT
  CCGGCAAAGCCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000530 unedited
  GGGCTAAATGTCATGTTTCTTATTCATTTAAGGACATTTGGNAGTTTGNGTAATAAATAT
  ACAGCAATGTAGCAAAAATCCTAATAATCCCCTGTGGCTGCAGTGCATCCTCTTCCTCCC
  CCCCGCCCCCCCATTTCCCATTTGTTTTTCCTGCTTTTTGTTTGGTTGTTTAAAAACCAT
  TTCTTAAGGCTCATTTGCCCCCTCCCCCACTCCCTGACCTATGATGATCCATTCTCTCTC
  TCTGTCTCTCTCCTCAAGACCTGGAGTCCCAGAACAGTGCTCCAAAGTGGTCAGGGGGAA
  CCAATGCTATACTGAAGTGTGAGGATCAGGGGCCCCTCTACTTGGGCTGGCTCTTCATTA
  AGGACTTAAGGGGGGCGTGAATTGCAAAAGCCACATGACGTCATTGGTCCTCAGTCATGG
  GTGTAAGGGCAGAGGATGAGCACAGGTAAACAAACAAAGAATGGATTAAAAAATAAAAAA
  TAATAACCTAAATAAACCCTGAAGGATAGTCTGATCTGGGTGCTCTGGGTATTAGCTGGG
  GGGACAGAGTATGGAGCTGGGGAATGAATTCTGGAATGAAACTTACATCTCAAAGGGAGG
  TGAGGGCAGGGCATGGCGGGGGAGCAAAGAGGGAAAGCACCTAGACGGGGGTAAGATGAG
  CCTATGTCCCCCTGCTCTGGCAGGGCCTGGGGTGGGGGGGTGGCGATCACTTGTCCGAGT
  TCAGGCCCATCATGTTCTTGATGGCGTTTTTGAAGCTGGTTCTGCTGGGGGACTTGACAG
  CAGGCCCGAGCTCCGGGCTCTGCTCATCCTTTCGTAGCTCCATCTCGATGACCATCACCT
  TTGGGCCTTTTGCGGACTCCACCCCTTACCCCCGATCCCNCGCCCAGGCCCGCTAACCGC
  TATTTCTTATCCTTGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_000530
• Insert Size: 1720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000530.3, NP_000521.1
• RefSeq Size: 1718 bp
• RefSeq ORF: 777 bp
• Locus ID: 4359
• Cytogenetics: 1q23.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: 'This gene is specifically expressed in Schwann cells of the peripheral nervous system and encodes a type I transmembrane glycoprotein that is a major structural protein of the peripheral myelin sheath. The encoded protein contains a large hydrophobic extracellular domain and a smaller basic intracellular domain, which are essential for the formation and stabilization of the multilamellar structure of the compact myelin. Mutations in this gene are associated with autosomal dominant form of Charcot-Marie-Tooth disease type 1 (CMT1B) and other polyneuropathies, such as Dejerine-Sottas syndrome (DSS) and congenital hypomyelinating neuropathy (CHN). A recent study showed that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MPZ, also known as P0) results from translation termination at the upstream UAG stop codon, while the longer isoform (L-MPZ) results from UAG stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MPZ).