Dopamine beta Hydroxylase (DBH) (NM_000787) Human Untagged Clone

CAT#: SC122575

DBH (untagged)-Human dopamine beta-hydroxylase (dopamine beta-monooxygenase) (DBH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DBH
• Synonyms: DBM; ORTHYP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000787 edited
  CGCCCTCAGTCGCTGGGCCAGCCTGCCCGGCCCCAGCATGCGGGAGGCAGCCTTCATGTA
  CAGCACAGCAGTGGCCATCTTCCTGGTCATCCTGGTGGCCGCACTGCAGGGCTCGGCTCC
  CCGTGAGAGCCCCCTCCCCTATCACATCCCCCTGGACCCGGAGGGGTCCCTGGAGCTCTC
  ATGGAATGTCAGCTACACCCAGGAGGCCATCCATTTCCAGCTCCTGGTGCGGAGGCTCAA
  GGCTGGCGTCCTGTTTGGGATGTCCGACCGTGGCGAGCTTGAGAACGCAGATCTCGTGGT
  GCTCTGGACCGATGGGGACACTGCCTATTTTGCGGACGCCTGGAGTGACCAGAAGGGGCA
  GATCCACCTGGATCCCCAGCAGGACTACCAGCTGCTGCAGGTGCAGAGGACCCCAGAAGG
  CCTGACCCTGCTTTTCAAGAGGCCCTTTGGCACCTGCGACCCCAAGGATTACCTCATTGA
  GGACGGCACTGTCCACTTGGTCTACGGGATCCTGGAGGAGCCGTTCCGGTCACTGGAGGC
  CATCAACGGCTCGGGCCTGCAGATGGGGCTGCAGAGGGTGCAGCTCCTGAAGCCCAATAT
  CCCCGAACCGGAGTTGCCCTCAGACGCGTGCACCATGGAGGTCCAAGCTCCCAATATCCA
  GATCCCCAGCCAGGAGACCACGTACTGGTGCTACATTAAGGAGCTTCCAAAGGGCTTCTC
  TCGGCACCACATTATCAAGTACGAGCCCATCGTCACCAAGGGCAATGAGGCCCTTGTCCA
  CCACATGGAAGTCTTCCAGTGCGCCCCCGAGATGGACAGCGTCCCCCACTTCAGCGGGCC
  CTGCGACTCCAAGATGAAACCCGACCGCCTCAACTACTGCCGCCACGTGCTGGCCGCCTG
  GGCCCTGGGTGCCAAGGCATTTTACTACCCAGAGGAAGCCGGCCTTGCCTTCGGGGGTCC
  AGGGTCCTCCAGATATCTCCGCCTGGAAGTTCACTACCACAACCCACTGGTGATAGAAGG
  ACGAAACGACTCCTCAGGCATCCGCTTGTACTACACAGCCAAGCTGCGGCGCTTCAACGC
  GGGGATCATGGAGCTGGGACTGGTGTACACGCCAGTGATGGCCATTCCACCACGGGAGAC
  CGCCTTCATCCTCACTGGCTACTGCACGGACAAGTGCACCCAGCTGGCACTGCCTCCCTC
  CGGGATCCACATCTTCGCCTCTCAGCTCCACACACACCTGACTGGGAGAAAGGTGGTCAC
  AGTGCTGGTCCGGGACGGCCGGGAGTGGGAGATCGTGAACCAGGACAATCACTACAGCCC
  TCACTTCCAGGAGATCCGCATGTTGAAGAAGGTCGTGTCGGTCCATCCGGGAGATGTGCT
  CATCACCTCCTGCACGTACAACACGGAAGACCGGGAGCTGGCCACAGTGGGGGGCTTCGG
  GATCCTGGAGGAGATGTGTGTCAACTACGTGCACTACTACCCCCAGACGCAGCTGGAGCT
  CTGCAAGAGCGCTGTGGACGCCGGCTTCCTGCAGAAGTACTTCCACCTCATCAACAGGTT
  CAACAACGAGGATGTCTGCACCTGCCCTCAGGCGTCCGTGTCTCAGCAGTTCACCTCTGT
  TCCCTGGAACTCCTTCAACCGCGACGTACTGAAGGCCCTGTACAGCTTCGCGCCCATCTC
  CATGCACTGCAACAAGTCCTCAGCCGTCCGCTTCCAGGGTGAATGGAACCTGCAGCCCCT
  GCCCAAGGTCATCTCCACACTGGAAGAGCCCACCCCACAGTGCCCCACCAGCCAGGGCCG
  AAGCCCTGCTGGCCCCACCGTTGTCAGCATTGGTGGGGGCAAAGGCTGAGGGGGGACCTA
  CTCCTCCCCCTCCTCCATGCTGTCCCTGTGGGCTCACACCGGCACTGTGCACTCTACTCT
  GCGACGATCCCCATGGAACAGCCCTGCATGCCCAGGATGAAGGGGCCAGACCACGCCCCT
  GCCTGAGACCACGGTCCAATCCAGCCTTCTTCCCCCAGGGTCCCCTGCATGGCTGAGAGG
  GTGTGGGTGCCCTGTTGACCTACCCTGGACCGAGTGGACCACGACCTCGTCCATTTAAAC
  CCGGCTGACTCAGTGCAGGGACAGCCTGCACAGTGGTCCAGGGTCCAGCCCTCCGCCAGC
  CCTGTTCCGCCTCACTGGGTGTGGCCTGGCTTCTGGGACAGGCACCATGCTGGGCCGGGG
  TGTGGAATCACCGGGAACGCCCCCGCCCCCGCCCCGCTGCTCCCGGTGTGCAGCGGGTGC
  GGGTGCCGCTTAAACATTTCCCTGCTGAGTGGCTCGTGTTTCACAGTGGGCGGCTTCCCT
  GCGACGGAGGCAGGACCAGGCATTTAGCTAGTTAGAGACTCGCCTGGGAAATTGCTCCAT
  TCCTGAGTAAACAGATATTTTCGCCCACCTAAAGGGAAGCCCTGACAACAACTATCACCA
  AAAGACGAGGCGGCAAAGATCCAGCGGGGCTTCTGGGCGCCGGTTCCACGTGGGGTGGAA
  TTATTAGCACCAGCTTGCTTCTCTGCCGGTGGGGCCAGCGCTGAACAGACCGGGGTGGAG
  TCAGGGCTGTGCTTTCCGCGTGGTTCTGCCACTTAGGGAGTGTGCCTTGGGCGGGCCATT
  TCACATTCCTGACCCTCACTTTTCTCATCTGTAAAACCAGGCTGATGCCGTGCGGGCTAA
  TGAGCCAATAAAGCTCACACTTGGGCTGGCACCCACTGGAGGTGGGAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000787 unedited
  AGTTAGGATTTGTATACGACTTACTATAGGCGGCCGCGAATTCGCACGAGGCGCCCTCAG
  TCGCTGGGCCAGCCTGCCCGGCCCCAGCATGCGGGAGGCAGCCTTCATGTACAGCACAGC
  AGTGGCCATCTTCCTGGTCATCCTGGTGGCCGCACTGCAGGGCTCGGCTCCCCGTGAGAG
  CCCCCTCCCCTATCACATCCCCCTGGACCCGGAGGGGTCCCTGGAGCTCTCATGGAATGT
  CAGCTACACCCAGGAGGCCATCCATTTCCAGCTCCTGGTGCGGAGGCTCAAGGCTGGCGT
  CCTGTTTGGGATGTCCGACCGTGGCGAGCTTGAGAACGCAGATCTCGTGGTGCTCTGGAC
  CGATGGGGACACTGCCTATTTTGCGGACGCCTGGAGTGACCAGAAGGGGCAGATCCACCT
  GGATCCCCAGCAGGACTACCAGCTGCTGCAGGTGCAGAGGACCCCAGAAGGCCTGACCCT
  GCTTTTCAAGAGGCCCTTTGGCACCTGCGACCCCAAGGATTACCTCATTGAGGACGGCAC
  TGTCCACTTGGTCTACGGGATCCTGGAGGAGCCGTTCCGGTCACTGGAGGCCATCAACGG
  CTCGGGCCTGCAGATGGGGCTGCAGAGGGTGCAGCTCCTGAAACCCAATATCCCCGAACC
  GGAGTTGCCCTCAGACGCGTGCACCATGGAGGTCCAAGCTTCCAATATCCAGATCCCCAG
  CCAGGAACCACGTTCTGGTGCTACATTAAGGAGCCTTCAAAGGGCTTCTCTCGGCACCCC
  ATATCCAGTTCAAACCCTTCGTCCCAAGGGCAAGGAAGACCCTGTCCACCCCTTGGAAAT
  CTTCCTTGCGCCCCGAAAAGGGAACGGGTCCCCATTTAGGCGGGCCCTGGAACTCCAAAA
  GAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000787
• Insert Size: 2807 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000787.2, NP_000778.2
• RefSeq Size: 2760 bp
• RefSeq ORF: 1812 bp
• Locus ID: 1621
• Cytogenetics: 9q34.2
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Metabolic pathways, Tyrosine metabolism
• Gene Summary: 'The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]'