Dopamine beta Hydroxylase (DBH) (NM_000787) Human Untagged Clone

CAT#: SC318060

DBH (untagged)-Human dopamine beta-hydroxylase (dopamine beta-monooxygenase) (DBH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DBH
• Synonyms: DBM; ORTHYP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000787 edited
  ATGCCCGCCCTCAGTCGCTGGGCCAGCCTGCCCGGCCCCAGCATGCGGGAGGCAGCCTTC
  ATGTACAGCACAGCAGTGGCCATCTTCCTGGTCATCCTGGTGGCCGCACTGCAGGGCTCG
  GCTCCCCGTGAGAGCCCCCTCCCCTATCACATCCCCCTGGACCCGGAGGGGTCCCTGGAG
  CTCTCATGGAATGTCAGCTACACCCAGGAGGCCATCCATTTCCAGCTCCTGGTGCGGAGG
  CTCAAGGCTGGCGTCCTGTTTGGGATGTCCGACCGTGGCGAGCTTGAGAACGCAGATCTC
  GTGGTGCTCTGGACCGATGGGGACACTGCCTATTTTGCGGACGCCTGGAGTGACCAGAAG
  GGGCAGATCCACCTGGATCCCCAGCAGGACTACCAGCTGCTGCAGGTGCAGAGGACCCCA
  GAAGGCCTGACCCTGCTTTTCAAGAGGCCCTTTGGCACCTGCGACCCCAAGGATTACCTC
  ATTGAGGACGGCACTGTCCACTTGGTCTACGGGATCCTGGAGGAGCCGTTCCGGTCACTG
  GAGGCCATCAACGGCTCGGGCCTGCAGATGGGGCTGCAGAGGGTGCAGCTCCTGAAGCCC
  AATATCCCCGAACCGGAGTTGCCCTCAGACGCGTGCACCATGGAGGTCCAAGCTCCCAAT
  ATCCAGATCCCCAGCCAGGAGACCACGTACTGGTGCTACATTAAGGAGCTTCCAAAGGGC
  TTCTCTCGGCACCACATTATCAAGTACGAGCCCATCGTCACCAAGGGCAATGAGGCCCTT
  GTCCACCACATGGAAGTCTTCCAGTGCGCCCCCGAGATGGACAGCGTCCCCCACTTCAGC
  GGGCCCTGCGACTCCAAGATGAAACCCGACCGCCTCAACTACTGCCGCCACGTGCTGGCC
  GCCTGGGCCCTGGGTGCCAAGGCATTTTACTACCCAGAGGAAGCCGGCCTTGCCTTCGGG
  GGTCCAGGGTCCTCCAGATATCTCCGCCTGGAAGTTCACTACCACAACCCACTGGTGATA
  GAAGGACGAAACGACTCCTCAGGCATCCGCTTGTACTACACAGCCAAGCTGCGGCGCTTC
  AACGCGGGGATCATGGAGCTGGGACTGGTGTACACGCCAGTGATGGCCATTCCACCACGG
  GAGACCGCCTTCATCCTCACTGGCTACTGCACGGACAAGTGCACCCAGCTGGCACTGCCT
  CCCTCCGGGATCCACATCTTCGCCTCTCAGCTCCACACACACCTGACTGGGAGAAAGGTG
  GTCACAGTGCTGGTCCGGGACGGCCGGGAGTGGGAGATCGTGAACCAGGACAATCACTAC
  AGCCCTCACTTCCAGGAGATCCGCATGTTGAAGAAGGTCGTGTCGGTCCATCCGGGAGAT
  GTGCTCATCACCTCCTGCACGTACAACACGGAAGACCGGGAGCTGGCCACAGTGGGGGGC
  TTCGGGATCCTGGAGGAGATGTGTGTCAACTACGTGCACTACTACCCCCAGACGCAGCTG
  GAGCTCTGCAAGAGCGCTGTGGACGCCGGCTTCCTGCAGAAGTACTTCCACCTCATCAAC
  AGGTTCAACAACGAGGATGTCTGCACCTGCCCTCAGGCGTCCGTGTCTCAGCAGTTCACC
  TCTGTTCCCTGGAACTCCTTCAACCGCGACGTACTGAAGGCCCTGTACAGCTTCGCGCCC
  ATCTCCATGCACTGCAACAAGTCCTCAGCCGTCCGCTTCCAGGGTGAATGGAACCTGCAG
  CCCCTGCCCAAGGTCATCTCCACACTGGAAGAGCCCACCCCACAGTGCCCCACCAGCCAG
  GGCCGAAGCCCTGCTGGCCCCACCGTTGTCAGCATTGGTGGGGGCAAAGGCTGAGGGGGG
  ACCTACTCCTCCCCCTCCTCCATGCTGTCCCTGTGGGCTCACACCGGCACTGTGCACTCT
  ACTCTGCGACGATCCCCATGGAACAGCCCTGCATGCCCAGGATGAAGGGGCCAGACCACG
  CCCCTGCCTGAGACCACGGTCCAATCCAGCCTTCTTCCCCCAGGGTCCCCTGCATGGCTG
  AGAGGGTGTGGGTGCCCTGTTGACCTACCCTGGACCGAGTGGACCACGACCTCGTCCATT
  TAAACCCGGCTGACTCAGTGCAGGGACAGCCTGCACAGTGGTCCAGGGTCCAGCCCTCCG
  CCAGCCCTGTTCCGCCTCACTGGGTGTGGCCTGGCTTCTGGGACAGGCACCATGCTGGGC
  CGGGGTGTGGAATCACCGGGAACGCCCCCGCCCCCGCCCCGCTGCTCCCGGTGTGCAGCG
  GGTGCGGGTGCCGCTTAAACATTTCCCTGCTGAGTGGCTCGTGTTTCACAGTGGGCGGCT
  TCCCTGCGACGGAGGCAGGACCAGGCATTTAGCTAGTTAGAGACTCGCCTGGGAAATTGC
  TCCATTCCTGAGTAAACAGATATTTTCGCCCACCTAAAGGGAAGCCCTGACAACAACTAT
  CACCAAAAGACGAGGCGGCAAAGATCCAGCGGGGCTTCTGGGCGCCGGTTCCACGTGGGG
  TGGAATTATTAGCACCAGCTTGCTTCTCTGCCGGTGGGGCCAGCGCTGAACAGACCGGGG
  TGGAGTCAGGGCTGTGCTTTCCGCGTGGTTCTGCCACTTAGGGAGTGTGCCTTGGGCGGG
  CCATTTCACATTCCTGACCCTCACTTTTCTCATCTGTAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000787
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000787.3, NP_000778.3
• RefSeq Size: 2812 bp
• RefSeq ORF: 1854 bp
• Locus ID: 1621
• Cytogenetics: 9q34.2
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Metabolic pathways, Tyrosine metabolism
• Gene Summary: 'The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]'