PCB (PC) (NM_000920) Human Untagged Clone

CAT#: SC122580

PC (untagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PC
• Synonyms: PCB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000920 edited
  CAGCAGCGGTAGAGGCGGCGGCGAGGACTGGCGACGGCGAGGAGATAGTGTCTGCCTTCT
  GGAGAGCTGACCAAACACTAAGGATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGC
  TCCTGGGAATCCGCCGAACCTCCACCGCCCCCGCTGCCTCCCCAAATGTCCGGCGCCTGG
  AGTATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGAGATTGCCATCCGTGTGT
  TCCGGGCCTGCACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAGCAGGACACGG
  GCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCCG
  TGCAGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATG
  CAGTGCACCCTGGCTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGG
  ATGCAGGGGTCCGGTTTATTGGGCCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGG
  TGGAGGCCCGGGCCATCGCCATTGCTGCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCC
  CCATCACGTCCCTGCATGAGGCCCACGAGTTCTCCAACACCTACGGCTTCCCCATCATCT
  TCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTGCACAGCTACGAGGAGC
  TGGAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGC
  TGTTTGTGGAGAAGTTCATCGAGAAGCCACGGCACATCGAGGTGCAGATCTTGGGGGACC
  AGTATGGGAACATCCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGGCGGCACCAGA
  AGGTGGTCGAGATTGCCCCCGCCGCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCA
  GCGACTCTGTGAAACTCGCTAAACAGGTGGGCTACGAGAACGCAGGCACCGTGGAGTTCC
  TGGTGGACAGGCACGGCAAGCACTACTTCATCGAGGTCAACTCCCGCCTGCAGGTGGAGC
  ACACGGTCACAGAGGAGATCACCGACGTAGACCTGGTCCATGCTCAGATCCACGTGGCTG
  AGGGCAGGAGCCTACCCGACCTGGGCCTGCGGCAGGAGAACATCCGCATCAACGGGTGTG
  CCATCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGCTTCCAGCCGGACACCGGCC
  GCATTGAGGTGTTCCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCT
  TCCAAGGAGCCGTCATCTCGCCCCACTACGACTCCCTGCTGGTCAAAGTCATTGCCCACG
  GCAAAGACCACCCCACGGCCGCCACCAAGATGAGCAGGGCCCTTGCGGAGTTCCGCGTCC
  GAGGTGTGAAGACCAACATCGCCTTCCTGCAGAATGTGCTCAACAACCAGCAGTTCCTGG
  CAGGCACTGTGGACACCCAGTTCATCGACGAGAACCCAGAGCTGTTCCAGCTGCGGCCTG
  CACAGAACCGGGCCCAAAAGCTGTTGCACTACCTCGGCCATGTCATGGTAAACGGTCCAA
  CCACCCCGATTCCCGTCAAGGCCAGCCCCAGCCCCACGGACCCCGTTGTCCCTGCAGTGC
  CCATAGGCCCGCCCCCGGCTGGTTTCAGAGACATCCTGCTGCGAGAGGGGCCTGAGGGCT
  TTGCTCGAGCTGTGCGGAACCACCCGGGGCTGCTGCTGATGGACACGACCTTCAGGGACG
  CCCACCAGTCACTGCTGGCCACTCGTGTGCGCACCCACGATCTCAAAAAGATCGCCCCCT
  ATGTTGCCCACAACTTCAGCAAGCTCTTCAGCATGGAGAACTGGGGAGGAGCCACGTTTG
  ACGTCGCCATGCGCTTCCTGTATGAGTGCCCCTGGCGGCGGCTGCAGGAGCTCCGGGAGC
  TCATCCCCAACATCCCTTTCCAGATGCTGCTGCGGGGGGCCAATGCTGTGGGCTACACCA
  ACTACCCAGACAACGTGGTCTTCAAGTTCTGTGAAGTGGCCAAAGAGAATGGCATGGATG
  TCTTCCGTGTGTTTGACTCCCTCAACTACTTGCCCAACATGCTGCTGGGCATGGAGGCGG
  CAGGAAGTGCCGGAGGCGTGGTGGAGGCTGCCATCTCATACACGGGCGACGTGGCCGACC
  CCAGCCGCACCAAGTACTCACTGCAGTACTACATGGGCTTGGCCGAAGAGCTGGTGCGAG
  CTGGCACCCACATCCTGTGCATCAAGGACATGGCCGGGCTGCTGAAGCCCACGGCCTGCA
  CCATGCTGGTCAGCTCCCTCCGGGACCGCTTCCCCGACCTCCCACTGCACATCCACACCC
  ACGACACGTCAGGGGCAGGCGTGGCAGCCATGCTGGCCTGTGCCCAGGCTGGAGCTGATG
  TGGTGGATGTGGCAGCTGATTCCATGTCTGGGATGACTTCACAGCCCAGCATGGGGGCCC
  TGGTGGCCTGTACCAGAGGGACTCCCCTGGACACAGAGGTGCCCATGGAGCGCGTGTTTG
  ACTACAGTGAGTACTGGGAGGGGGCTCGGGGACTGTACGCGGCCTTCGACTGCACGGCCA
  CCATGAAGTCTGGCAACTCGGACGTGTATGAAAATGAGATCCCAGGGGGCCAGTACACCA
  ACCTGCACTTCCAGGCCCACAGCATGGGGCTTGGCTCCAAGTTCAAGGAGGTCAAGAAGG
  CCTATGTGGAGGCCAACCAGATGCTGGGCGATCTCATCAAGGTGACGCCCTCCTCCAAGA
  TCGTGGGGGACCTGGCCCAGTTTATGGTGCAGAATGGATTGAGCCGGGCAGAGGCCGAAG
  CTCAGGCGGAAGAGCTGTCCTTTCCCCGCTCCGTGGTGGAGTTCCTGCAGGGCTACATCG
  GTGTCCCCCATGGGGGGTTCCCCGAACCCTTTCGCTCTAAGGTACTGAAGGACCTGCCAA
  GGGTGGAGGGGCGGCCTGGAGCCTCCCTCCCTCCCCTGGATCTGCAGGCACTGGAGAAGG
  AGCTGGTAGACCGGCATGGGGAGGAGGTGACGCCGGAAGATGTGCTCTCAGCAGCTATGT
  ACCCCGATGTGTTTGCCCACTTCAAGGACTTCACTGCCACCTTTGGCCCCCTGGATAGCC
  TGAATACTCGCCTCTTCCTGCAGGGACCCAAGATCGCAGAGGAGTTTGAGGTGGAGCTGG
  AGCGGGGCAAGACGCTGCACATCAAAGCCCTGGCCGTGAGCGACCTGAACCGGGCCGGCC
  AGAGGCAGGTCTTCTTTGAGCTCAATGGGCAGCTGCGGTCCATCTTGGTCAAGGACACCC
  AGGCCATGAAGGAGATGCACTTCCACCCCAAGGCCCTAAAGGACGTGAAGGGCCAGATCG
  GGGCGCCCATGCCTGGGAAGGTGATAGACATCAAAGTGGTGGCAGGGGCCAAGGTGGCCA
  AGGGCCAGCCCCTGTGTGTGCTCAGTGCCATGAAGATGGAGACTGTGGTGACCTCACCCA
  TGGAGGGTACTGTCCGCAAGGTTCATGTGACCAAGGACATGACACTGGAAGGTGACGACC
  TCATCCTGGAGATCGAGTGATCTTGCCCCAGACCGGCAGCCTGGCCATCCCCAAGCCTTC
  AACAGAAGCTGTGCTGCCACGGCAGGCCCAGGCCAGCCAGTGCCCGAGGCCAGGAAGGCC
  GGGCCGTGGAGGTCCTGTCCACAGCTGGACAGGAGAGACACCGCCTGCGGTGGTTCATTC
  CTTTCAGCCATCGTCCTTTCCTCCGGCGGACAGCTGCTTACATGTTCATCTCTTGCCAAA
  TAAGGGTCCCCTCCTCACTGGAGACTACAAGTGGTGGGTCAGGTGGTCCTAGGACCCAGG
  GGAGGTTTAGGGGTCCTATCTCCTGGGGGAAGGGGAGATCTAAGATGTCCCAGGTCCTGG
  GAAGTTTACTCAATAAAGCTGGCTTTCCCCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000920 unedited
  GAAGCGTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCAGCAG
  CGGTAGAGGCGGCGGCGAGGACTGGCGACGGCGAGGAGATAGTGTCTGCCTTCTGGAGAG
  CTGACCAAACACTAAGGATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGG
  GAATCCGCCGAACCTCCACCGCCCCCGCTGCCTCCCCAAATGTCCGGCGCCTGGAGTATA
  AGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGAGATTGCCATCCGTGTGTTCCGGG
  CCTGCACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAGCAGGACACGGGCCAGA
  TGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCCGTGCAGG
  CCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGC
  ACCCTGGCTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAG
  GGGTCCGGTTTATTGGGCCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGGTGGAGG
  CCCGGGCCATCGCCATTGCTGCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCCCCATCA
  CGTCCCTGCATGAGGCCCACGAGTTCTCCAACACCTACGGCTTCCCCATCATCTTCAAGG
  CGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTGCACAGCTACGAGGAGCTGGAGG
  AGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGCTGTTTG
  TGGAGAAGTTCATCGAGAAGCACGGCACATCGAGGTGCAGATCTTTGGGGACCAGTATGG
  GAACATCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_000920
• Insert Size: 4058 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000920.2, NP_000911.1
• RefSeq Size: 4111 bp
• RefSeq ORF: 3537 bp
• Locus ID: 5091
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pyruvate metabolism
• Gene Summary: 'This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) results from the use of a more distal promoter. Transcript variants 1-3 encode the same protein.