alpha 1 Fetoprotein (AFP) (NM_001134) Human Untagged Clone

CAT#: SC122582

AFP (untagged)-Human alpha-fetoprotein (AFP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AFP
• Synonyms: AFPD; FETA; HPAFP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001134 edited
  CCACTGCCAATAACAAAATAACTAGCAACCATGAAGTGGGTGGAATCAATTTTTTTAATT
  TTCCTACTAAATTTTACTGAATCCAGAACACTGCATAGAAATGAATATGGAATAGCTTCC
  ATATTGGATTCTTACCAATGTACTGCAGAGATAAGTTTAGCTGACCTGGCTACCATATTT
  TTTGCCCAGTTTGTTCAAGAAGCCACTTACAAGGAAGTAAGCAAAATGGTGAAAGATGCA
  TTGACTGCAATTGAGAAACCCACTGGAGATGAACAGTCTTCAGGGTGTTTAGAAAACCAG
  CTACCTGCCTTTCTGGAAGAACTTTGCCATGAGAAAGAAATTTTGGAGAAGTACGGACAT
  TCAGACTGCTGCAGCCAAAGTGAAGAGGGAAGACATAACTGTTTTCTTGCACACAAAAAG
  CCCACTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTGTCACAAGCTGTGAAGCA
  TATGAAGAAGACAGGGAGACATTCATGAACAAATTCATTTATGAGATAGCAAGAAGGCAT
  CCCTTCCTGTATGCACCTACAATTCTTCTTTGGGCTGCTCGCTATGACAAAATAATTCCA
  TCTTGCTGCAAAGCTGAAAATGCAGTTGAATGCTTCCAAACAAAGGCAGCAACAGTTACA
  AAAGAATTAAGAGAAAGCAGCTTGTTAAATCAACATGCATGTGCAGTAATGAAAAATTTT
  GGGACCCGAACTTTCCAAGCCATAACTGTTACTAAACTGAGTCAGAAGTTTACCAAAGTT
  AATTTTACTGAAATCCAGAAACTAGTCCTGGATGTGGCCCATGTACATGAGCACTGTTGC
  AGAGGAGATGTGCTGGATTGTCTGCAGGATGGGGAAAAAATCATGTCCTACATATGTTCT
  CAACAAGACACTCTGTCAAACAAAATAACAGAATGCTGCAAACTGACCACGCTGGAACGT
  GGTCAATGTATAATTCATGCAGAAAATGATGAAAAACCTGAAGGTCTATCTCCAAATCTA
  AACAGGTTTTTAGGAGATAGAGATTTTAACCAATTTTCTTCAGGGGAAAAAAATATCTTC
  TTGGCAAGTTTTGTTCATGAATATTCAAGAAGACATCCTCAGCTTGCTGTCTCAGTAATT
  CTAAGAGTTGCTAAAGGATACCAGGAGTTATTGGAGAAGTGTTTCCAGACTGAAAACCCT
  CTTGAATGCCAAGATAAAGGAGAAGAAGAATTACAGAAATACATCCAGGAGAGCCAAGCA
  TTGGCAAAGCGAAGCTGCGGCCTCTTCCAGAAACTAGGAGAATATTACTTACAAAATGCG
  TTTCTCGTTGCTTACACAAAGAAAGCCCCCCAGCTGACCTCGTCGGAGCTGATGGCCATC
  ACCAGAAAAATGGCAGCCACAGCAGCCACTTGTTGCCAACTCAGTGAGGACAAACTATTG
  GCCTGTGGCGAGGGAGCGGCTGACATTATTATCGGACACTTATGTATCAGACATGAAATG
  ACTCCAGTAAACCCTGGTGTTGGCCAGTGCTGCACTTCTTCATATGCCAACAGGAGGCCA
  TGCTTCAGCAGCTTGGTGGTGGATGAAACATATGTCCCTCCTGCATTCTCTGATGACAAG
  TTCATTTTCCATAAGGATCTGTGCCAAGCTCAGGGTGTAGCGCTGCAAACGATGAAGCAA
  GAGTTTCTCATTAACCTTGTGAAGCAAAAGCCACAAATAACAGAGGAACAACTTGAGGCT
  GTCATTGCAGATTTCTCAGGCCTGTTGGAGAAATGCTGCCAAGGCCAGGAACAGGAAGTC
  TGCTTTGCTGAAGAGGGACAAAAACTGATTTCAAAAACTCGTGCTGCTTTGGGAGTTTAA
  ATTACTTCAGGGGAAGAGAAGACAAAACGAGTCTTTCATTCGGTGTGAACTTTTCTCTTT
  AATTTTAACTGATTTAACACTTTTTGTGAATTAATGAAATGATAAAGACTTTTATGTGAG
  ATTTCCTTATCACAGAAATAAAATATCTCCAAATGTTAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001134 unedited
  NGGTAACGTCAATATTTGTATACGACTCACTATAGGCGGCCGCGNATTCCCGGGATCCAC
  TGCCAATAACAAAATAACTAGCAACCATGAAGTGGGTGGAATCAATTTTTTTAATTTTCC
  TACTAAATTTTACTGAATCCAGAACACTGCATAGAAATGAATATGGAATAGCTTCCATAT
  TGGATTCTTACCAATGTACTGCAGAGATAAGTTTAGCTGACCTGGCTACCATATTTTTTG
  CCCAGTTTGTTCAAGAAGCCACTTACAAGGAAGTAAGCAAAATGGTGAAAGATGCATTGA
  CTGCAATTGAGAAACCCACTGGAGATGAACAGTCTTCAGGGTGTTTAGAAAACCAGCTAC
  CTGCCTTTCTGGAAGAACTTTGCCATGAGAAAGAAATTTTGGAGAAGTACGGACATTCAG
  ACTGCTGCAGCCAAAGTGAAGAGGGAAGACATAACTGTTTTCTTGCACACAAAAAGCCCA
  CTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTGTCACAAGCTGTGAAGCATATG
  AAGAAGACAGGGAGACATTCATGAACAAATTCATTTATGAGATAGCAAGAAGGCATCCCT
  TCCTGTATGCACCTACAATTCTTCTTTGGGCTGCTCGCTATGACAAAATAATTCCATCTT
  GCTGCAAAGCTGAANATGCAGTTGAATGCCTTCCAACAAGAGCAGCAACAGTTACAAAAG
  ATTTAAGAGAAGCAGCTTGTTAAATCACATGCATGTGCAGTAATGAAAAAATTTGGGACC
  CGAACTTTCCAGCCTTACTGTTACTAAACTGAATCAGAGTTTACCACAGTTAATTTACTG
  AATCCAAAACTAGTCCGGGAGTGGCCATGTTCTGAACACG
  
• Restriction Sites: Please inquire
• ACCN: NM_001134
• Insert Size: 2098 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134.1, NP_001125.1
• RefSeq Size: 2032 bp
• RefSeq ORF: 1830 bp
• Locus ID: 174
• Cytogenetics: 4q13.3
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).