CLPS (NM_001832) Human Untagged Clone

CAT#: SC122609

CLPS (untagged)-Human colipase, pancreatic (CLPS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLPS
• Synonyms: colipase; colipase, pancreatic; OTTHUMP00000016271; pancreatic colipase preproprotein
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001832 edited
  GTCTCCCGCCACCCACACCAGCTGTCCCACTCGCCATGGAGAAGATCCTGATCCTCCTGC
  TTGTCGCCCTCTCTGTGGCCTATGCAGCTCCTGGCCCCCGGGGGATCATTATCAACCTGG
  AGAACGGTGAGCTCTGCATGAATAGTGCCCAGTGTAAGAGCAATTGCTGCCAGCATTCAA
  GTGCGCTGGGCCTGGCCCGCTGCACATCCATGGCCAGCGAGAACAGCGAGTGCTCTGTCA
  AGACGCTCTATGGGATTTACTACAAGTGTCCCTGTGAGCGTGGCCTGACCTGTGAGGGAG
  ACAAGACCATCGTGGGCTCCATCACCAACACCAACTTTGGCATCTGCCATGACGCTGGAC
  GCTCCAAGCAGTGAGACTGCCCACCCACTCCCACACCTAGCCCAGAATGCTGTAGGCCAC
  TAGGCGCAGGGGCATCTCTCCCCTGCTCCAGCGCATCTCCCGGGCTGGCCACCTCCTTGA
  CCAGCATATCTGTTTTCTGATTGCGCTCTTCACAATTAAAGGCCTCCTGCAAACCGTAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001832 unedited
  NNNCCGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCCATTACGGCCG
  GGGGTCTCCCGCCACCCACACCAGCTGTCCCACTCGCCATGGAGAAGATCCTGATCCTCC
  TGCTTGTCGCCCTCTCTGTGGCCTATGCAGCTCCTGGCCCCCGGGGGATCATTATCAACC
  TGGAGAACGGTGAGCTCTGCATGAATAGTGCCCAGTGTAAGAGCAATTGCTGCCAGCATT
  CAAGTGCGCTGGGCCTGGCCCGCTGCACATCCATGGCCAGCGAGAACAGCGAGTGCTCTG
  TCAAGACGCTCTATGGGATTTACTACAAGTGTCCCTGTGAGCGTGGCCTGACCTGTGAGG
  GAGACAAGACCATCGTGGGCTCCATCACCAACACCAACTTTGGCATCTGCCATGACGCTG
  GACGCTCCAAGCAGTGAGACTGCCCACCCACTCCCACACCTAGCCCAGAATGCTGTAGGC
  CACTAGGCGCAGGGGCATCTCTCCCCTGCTCCAGCGCATCTCCCGGGCTGGCCACCTCCT
  TGACCAGCATATCTGTTTTCTGATTGCGCTCTTCACAATTAAAGGCCTCCTGCAAACCGT
  AAAAAANAAAAAAAAAAAAAAAAAAAAAAAACATGTTCGGCCGCCTCGGCCCTCGACTCT
  AGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACC
  CCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCC
  TAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAAATTATGGGTGG
  AGGGGGGGGTGGGGTTTGGANCAGGGGGCANNTTTGGAAAACAACCTGTAGGGGCTGGGG
  GGTCAATTGGGAAACCAAACTG
  
• Restriction Sites: Please inquire
• ACCN: NM_001832
• Insert Size: 568 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001832.2, NP_001823.1
• RefSeq Size: 539 bp
• RefSeq ORF: 339 bp
• Locus ID: 1208
• Cytogenetics: 6p21.31
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).