Kisspeptin (KISS1) (NM_002256) Human Untagged Clone

CAT#: SC122622

KISS1 (untagged)-Human KiSS-1 metastasis-suppressor (KISS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KISS1
• Synonyms: HH13; KiSS-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002256 edited
  CTCTTGAGACCGGGAGCCCAGCTGCCCACCCTCTGGACATTCACCCAGCCAGGTGGTCTC
  GTCACCTCAGAGGCTCCGCCAGACTCCTGCCCAGGCCAGGACTGAGGCAAGCCTCAAGGC
  ACTTCTAGGACCTGCCTCTTCTCACCAAGATGAACTCACTGGTTTCTTGGCAGCTACTGC
  TTTTCCTCTGTGCCACCCACTTTGGGGAGCCATTAGAAAAGGTGGCCTCTGTGGGGAATT
  CTAGACCCACAGGCCAGCAGCTAGAATCCCTGGGCCTCCTGGCCCCCGGGGAGCAGAGCC
  TGCCGTGCACCGAGAGGAAGCCAGCTGCTACTGCCAGGCTGAGCCGTCGGGGGACCTCGC
  TGTCCCCGCCCCCCGAGAGCTCCGGGAGCCCCCAGCAGCCGGGCCTGTCCGCCCCCCACA
  GCCGCCAGATCCCCGCACCCCAGGGCGCGGTGCTGGTGCAGCGGGAGAAGGACCTGCCGA
  ACTACAACTGGAACTCCTTCGGCCTGCGCTTCGGCAAGCGGGAGGCGGCACCAGGGAACC
  ACGGCAGAAGCGCTGGGCGGGGCTGAGGGCGCAGGTGCGGGGCAGTGAACTTCAGACCCC
  AAAGGAGTCAGAGCATGCGGGGCGGGGGCGGGGGGCGGGGACGTAGGGCTAAGGGAGGGG
  GCGCTGGAGCTTCCAACCCGAGGCAATAAAAGAAATGTTGCGTAACTCAAAAAAAAAAAA
  AAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002256 unedited
  NNNGGGGAAGCGTTCAAATATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCAC
  GAGGCTCTTGAGACCGGGAGCCCAGCTGCCCACCCTCTGGGCATTCACCCAGCCAGGTGG
  TCTCGTCACCTCAGAGGCTCCGCCAGACTCCTGCCCAGGCCAGGACTGAGGCAAGCCTCA
  AGGCACTTCTAGGACCTGCCTCTTCTCACCAAGATGAACTCACTGGTTTCTTGGCAGCTA
  CTGCTTTTCCTCTGTGCCACCCACTTTGGGGAGCCATTAGAAAAGGTGGCCTCTGTGGGG
  AATTCTAGACCCACAGGCCAGCAGCTAGAATCCCTGGGCCTCCTGGCCCCCGGGGAGCAG
  AGCCTGCCGTGCACCGAGAGGAAGCCAGCTGCTACTGCCAGGCTGAGCCGTCGGGGGACC
  TCGCTGTCCCCGCCCCCCGAGAGCTCCGGGAGCCCCCAGCAGCCGGGCCTGTCCGCCCCC
  CACAGCCGCCAGATCCCCGCACCCCAGGGCGCGGTGCTGGTGCAGCGGGAGAAGGACCTG
  CCGAACTACAACTGGAACTCCTTCGGCCTGCGCTTCGGCAAGCGGGAGGCGGCACCAGGG
  AACCACGGCAGAAGCGCTGGGCGGGGCTGANGGCGCANGTGCGGGGCAGTGAACTTCAGA
  CCCCAAAGGAGTCAAANCATGCGGGGCGGGGGCGGGGGGCGGGGGACCTAGGGCTAAAGG
  AGGGGGCGCTGGAGCTTCCAACCCGAGGCATTAAAAGAAAGTTGCGTAACTCAAAAAAAA
  AAAAAAAACTCGACTCCTAATTGCGGCCGCGTCATTACTGGTTCCTGAACAGATCCCGGT
  GGGCATCCCTGGGACCCCTCCCCAAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_002256
• Insert Size: 726 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002256.2, NP_002247.2
• RefSeq Size: 725 bp
• RefSeq ORF: 438 bp
• Locus ID: 3814
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Mar 2012]'