PAFAH1B3 (NM_002573) Human Untagged Clone

CAT#: SC122630

PAFAH1B3 (untagged)-Human platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa) (PAFAH1B3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAFAH1B3
• Synonyms: PAFAHG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001145939 edited
  CGGGAAGCGCCGGGCGAGCCCACTGTGCGGCCGTCGTGGGGGAAGCGAAGGTTCCTGATT
  CCACCTACCTCCTAAGTGAAGGCTTCCGTCCCTGGAGAGGAGGCGGCGCCCTGTATCGGC
  CTTCGTCCTCCGCGGGTGTGCTAGCGTTGGGACGGTCCTTTGTTGCCGCGAGGGGTAGGA
  GTGGGCGTGGCGGAGCCAGCTCCGTTCGGAACACTCCCGGGCCGACCCGACTCGCTCATC
  CTGCAGGAGCTGCGGCGCCAAGATGAGTGGAGAGGAGAACCCAGCCAGCAAGCCCACGCC
  GGTGCAGGACGTACAGGGCGACGGGCGCTGGATGTCCCTGCACCATCGGTTCGTGGCTGA
  CAGCAAAGATAAGGAACCCGAAGTCGTCTTCATCGGGGACTCCTTGGTCCAGCTCATGCA
  CCAGTGCGAGATCTGGCGCGAGCTCTTCTCTCCTCTGCATGCACTTAACTTTGGCATTGG
  TGGTGACGGCACACAGCATGTACTGTGGCGGCTGGAGAATGGGGAGCTGGAACACATCCG
  GCCCAAGATTGTGGTGGTCTGGGTGGGCACCAACAACCACGGACACACAGCAGAGCAGGT
  GACTGGTGGCATCAAGGCCATTGTGCAACTGGTGAATGAGCGACAGCCCCAGGCCCGGGT
  TGTGGTGCTGGGCCTGCTTCCGCGAGGCCAACATCCCAACCCACTTCGGGAGAAGAACCG
  ACAGGTGAACGAGCTGGTACGGGCGGCACTGGCTGGCCACCCTCGGGCCCACTTCCTAGA
  TGCCGACCCTGGCTTTGTGCACTCAGATGGCACCATCAGCCATCATGACATGTATGATTA
  CCTGCATCTGAGCCGCCTGGGCTACACACCTGTTTGCCGGGCTCTGCACTCCCTGCTTCT
  GCGTCTGCTGGCCCAAGACCAGGGCCAAGGTGCTCCCCTGCTGGAGCCCGCACCCTAAGC
  ATCCTGCTGCCTTCCCACAACATTAAACTCTCCTTCCTCAGTGAAAAAAAAAAAAAAAAA
  A
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002573 unedited
  NAACGTCAACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGGAA
  GCGCCGGGCGAGCCCACTGTGCGGCCGTCGTGGGGGAAGCGAAGTTCCTGATTCCACCTA
  CCTCCTAAGTGAAGGCTTCCGTCCCTGGAGAGGAGGCGGCGCCCTGTATCGGCCTTCGTC
  CTCCGCGGGTGTGCTAGCGTTGGGACGGTCCTTTGTTGCCGCGAGGGGTAGGAGTGGGCG
  TGGCGGAGCCAGCTCCGTTCGGAACACTCCCGGGCCGACCCGACTCGCTCATCCTGCATG
  AGCTGCGGCGCCAAGATGAGTGGAGAGGAGAACCCAGCCAGCAAGCCCACGCCGGTGCAG
  GACGTACAGGGCGACGGGCGCTGGATGTCCCTGCACCATCGGTTCGTGGCTGACAGCAAA
  GATAAGGAACCCGAAGTCGTCTTCATCGGGGACTCCTTGGTCCAGCTCATGCACCAGTGC
  GAGATCTGGCGCGAGCTCTTCTCTCCTCTGCATGCACTTAACTTTGGCATTGGTGGTGAC
  GGCACACAGCATGTACTGTGGCGGCTGGAGAATGGGGAGCTGGAACACATCCGGCCCAAG
  ATTGTGGTGGTCTGGGTGGGCACCAACAACCACGGACACACAGCAGAGCAGGTGACTGGT
  GGCATCAAGGCCATTGTGCAACTGGTGAATGAGCGACAGCCCCAGGCCCGGGTTGTGGTG
  CTGGGCCTGCTTNCGCGAGGCCAACATCCCAACCCACTTCGGGAGAAAGACCGACAGGTG
  AACGAGCTGGTACGGGCGGCACTGGCTGGCCACCCTCGGGCCCACTTCCTAGATGCCGAC
  CCTGGCTTTGTGCACTCAGATGGCACCATCAGCCATCATGACATGTATGATTACCTGCAT
  CT
  
• Restriction Sites: Please inquire
• ACCN: NM_002573
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002573.2, NP_002564.1
• RefSeq Size: 1021 bp
• RefSeq ORF: 696 bp
• Locus ID: 5050
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Ether lipid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with cognitive disability, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.