Hyaluronidase PH20 (SPAM1) (NM_003117) Human Untagged Clone

CAT#: SC122646

SPAM1 (untagged)-Human sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPAM1
• Synonyms: HEL-S-96n; HYA1; HYAL1; HYAL3; HYAL5; PH-20; PH20; SPAG15
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003117 edited
  AGCGGAGGTGGTTAGCAGCACCTCATAAGGTCCTTCCTAGCAAGGGATGCTAATGACTAG
  CCAATGCTCTAGGAAGACATTGAGACCAGCCAACTTCTTGCCTTGATAACTACTGAAGAG
  ACATTGGGTGGCTGGATTTTGAAAGCAGACTTCTGGTTATAGGTGATGCAACTTGAAAAA
  CAATCCTGAAACATGAAACAAGAATAATAATATTTAAATGTAACTTAATCATTATACCTC
  TTTATCCATCAAAGTGAATTCATTCCATTCCCTTTCATCTGTGCTCATACTTTGCATCAG
  ATATTGGGTAAACCAAAGTGTGTAGGAAGAAATAAATGTTTTCATAGTCATTACTCTTTA
  CAATGGGAGTGCTAAAATTCAAGCACATCTTTTTCAGAAGCTTTGTTAAATCAAGTGGAG
  TATCCCAGATAGTTTTCACCTTCCTTCTGATTCCATGTTGCTTGACTCTGAATTTCAGAG
  CACCTCCTGTTATTCCAAATGTGCCTTTCCTCTGGGCCTGGAATGCCCCAAGTGAATTTT
  GTCTTGGAAAATTTGATGAGCCACTAGATATGAGCCTCTTCTCTTTCATAGGAAGCCCCC
  GAATAAACGCCACCGGGCAAGGTGTTACAATATTTTATGTTGATAGACTTGGCTACTATC
  CTTACATAGATTCAATCACAGGAGTAACTGTGAATGGAGGAATCCCCCAGAAGATTTCCT
  TACAAGACCATCTGGACAAAGCTAAGAAAGACATTACATTTTATATGCCAGTAGACAATT
  TGGGAATGGCTGTTATTGACTGGGAAGAATGGAGACCCACTTGGGCAAGAAACTGGAAAC
  CTAAAGATGTTTACAAGAATAGGTCTATTGAATTGGTTCAGCAACAAAATGTACAACTTA
  GTCTCACAGAGGCCACTGAGAAAGCAAAACAAGAATTTGAAAAAGCAGGGAAGGATTTCC
  TGGTAGAGACTATAAAATTGGGAAAATTACTTCGGCCAAATCACTTGTGGGGTTATTATC
  TTTTTCCGGATTGTTACAACCATCACTATAAGAAACCCGGTTACAATGGAAGTTGCTTCA
  ATGTAGAAATAAAAAGAAATGATGATCTCAGCTGGTTGTGGAATGAAAGCACTGCTCTTT
  ACCCATCCATTTATTTGAACACTCAGCAGTCTCCTGTAGCTGCTACACTCTATGTGCGCA
  ATCGAGTTCGGGAAGCCATCAGAGTTTCCAAAATACCTGATGCAAAAAGTCCACTTCCGG
  TTTTTGCATATACCCGCATAGTTTTTACTGATCAAGTTTTGAAATTCCTTTCTCAAGATG
  AACTTGTGTATACATTTGGCGAAACTGTTGCTCTGGGTGCTTCTGGAATTGTAATATGGG
  GAACCCTCAGTATAATGCGAAGTATGAAATCTTGCTTGCTCCTAGACAATTACATGGAGA
  CTATACTGAATCCTTACATAATCAACGTCACACTAGCAGCCAAAATGTGTAGCCAAGTGC
  TTTGCCAGGAGCAAGGAGTGTGTATAAGGAAAAACTGGAATTCAAGTGACTATCTTCACC
  TCAACCCAGATAATTTTGCTATTCAACTTGAGAAAGGTGGAAAGTTCACAGTACGTGGAA
  AACCGACACTTGAAGACCTGGAGCAATTTTCTGAAAAATTTTATTGCAGCTGTTATAGCA
  CCTTGAGTTGTAAGGAGAAAGCTGATGTAAAAGACACTGATGCTGTTGATGTGTGTATTG
  CTGATGGTGTCTGTATAGATGCTTTTCTAAAACCTCCCATGGAGACAGAAGAACCTCAAA
  TTTTCTACAATGCTTCACCCTCCACACTATCTGCCACAATGTTCATTTGGAGGCTGGAAG
  TCTGGGATCAAGGTATTAGCAGAATTGGTTTCTTCTGAGAGTCATGAGGGAAAAATGTGT
  TTCAGGCCTCTTCCCTTGGCTTACAGGAAATGAAAAAACCATGACTATCATCACCAACAT
  CCTTGGGTATTAAGTGCAGTCACTCTCCTAGATGCTGTGGGGAGAAGGCAAGTTACAAAG
  ATAGACCTTCCCTCAAGATAATCAGATTTTCATGGTATTATCCTTAACCTTTTTGACATC
  ATGGAGGCTTTGGGAATCTGATGAAGCCTATCAATTTTCTTCCAGAAGATATTTATATAA
  GATTATAAGAAAAATTATGTACACAGCTTATTTTATTGCATTGGATCAAAATGCCATTTA
  TAAAGAATTATGCCTTTTCCATCAATTTTAGCATGGAAAAATAATTTCAGGCAATATGCT
  TAAAAATTGGGGGAAGACAAAAGAAATCCATATCGTGTAAATAAAAATAAATTTTGGTTT
  TGCTCAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003117 unedited
  TCTGATATTTGTAATACGACTTCTTATAGGGCGGCCGCATAACTTCGTATAGCATACATT
  ATACTAATTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGGTGG
  TTAGCAGCACCTCATAAGGTCCTTCCTAGCAAGGGATGCTAATGACTAGCCAATGCTCTA
  GGAAGACATTGAGACCAGCCAACTTCTTGCCTTGATAACTACTGAAGAGACATTGGGTGG
  CTGGATTTTGAAAGCAGACTTCTGGTTATAGGTGATGCAACTTGAAAAACAATCCTGAAA
  CATGAAACAAGAATAATAATATTTAAATGTAACTTAATCATTATACCTCTTTATCCATCA
  AAGTGAATTCATTCCATTCCCTTTCATCTGTGCTCATACTTTGCATCAGATATTGGGTAA
  ACCAAAGTGTGTAGGAAGAAATAAATGTTTTCATAGTCATTACTCTTTACAATGGGAGTG
  CTAAAATTCAAGCACATCTTTTTCAGAAGCTTTGTTAAATCAAGTGGAGTATCCCAGATA
  GTTTTCACCTTCCTTCTGATTCCATGTTGCTTGACTCTGAATTTCAGAGCACCTCCTGTT
  ATTCCAAATGTGCCTTTCCTCTGGGCCTGGAATGCCCCAAGTGAATTNTGTCTTGGAAAA
  TTTGATGAGCCACTAGATATGAGCCTCTTCTCTTTCATAGGAAGCCCCCGAATAAACGCC
  ACCGGGCAAGGGTGTTACAATATTTTATGTTGATAGACTTGGCTACTATCCTTACATAGA
  TTCAATCACAGGAGTAACTGTGAATGGAGGAATCCNCCAGAAGATTTCCTTNACAGACCA
  TCTGGACAAGCTAAGAAAGACATACATTTTATATGCCAGTAGACATTTTGGAATGGGCTG
  TATGACTGGGAAGATGGAGACCCACTTGGGCAGAACTGGAAACTAAAGAGT
  
• Restriction Sites: Please inquire
• ACCN: NM_003117
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003117.3, NP_003108.2
• RefSeq Size: 2395 bp
• RefSeq ORF: 1536 bp
• Locus ID: 6677
• Cytogenetics: 7q31.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways
• Gene Summary: 'Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).