Hyaluronidase PH20 (SPAM1) (NM_003117) Human Untagged Clone

CAT#: SC122646

SPAM1 (untagged)-Human sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant 1


  "NM_003117" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SPAM1
Synonyms HEL-S-96n; HYA1; HYAL1; HYAL3; HYAL5; PH-20; PH20; SPAG15
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_003117 edited
AGCGGAGGTGGTTAGCAGCACCTCATAAGGTCCTTCCTAGCAAGGGATGCTAATGACTAG
CCAATGCTCTAGGAAGACATTGAGACCAGCCAACTTCTTGCCTTGATAACTACTGAAGAG
ACATTGGGTGGCTGGATTTTGAAAGCAGACTTCTGGTTATAGGTGATGCAACTTGAAAAA
CAATCCTGAAACATGAAACAAGAATAATAATATTTAAATGTAACTTAATCATTATACCTC
TTTATCCATCAAAGTGAATTCATTCCATTCCCTTTCATCTGTGCTCATACTTTGCATCAG
ATATTGGGTAAACCAAAGTGTGTAGGAAGAAATAAATGTTTTCATAGTCATTACTCTTTA
CAATGGGAGTGCTAAAATTCAAGCACATCTTTTTCAGAAGCTTTGTTAAATCAAGTGGAG
TATCCCAGATAGTTTTCACCTTCCTTCTGATTCCATGTTGCTTGACTCTGAATTTCAGAG
CACCTCCTGTTATTCCAAATGTGCCTTTCCTCTGGGCCTGGAATGCCCCAAGTGAATTTT
GTCTTGGAAAATTTGATGAGCCACTAGATATGAGCCTCTTCTCTTTCATAGGAAGCCCCC
GAATAAACGCCACCGGGCAAGGTGTTACAATATTTTATGTTGATAGACTTGGCTACTATC
CTTACATAGATTCAATCACAGGAGTAACTGTGAATGGAGGAATCCCCCAGAAGATTTCCT
TACAAGACCATCTGGACAAAGCTAAGAAAGACATTACATTTTATATGCCAGTAGACAATT
TGGGAATGGCTGTTATTGACTGGGAAGAATGGAGACCCACTTGGGCAAGAAACTGGAAAC
CTAAAGATGTTTACAAGAATAGGTCTATTGAATTGGTTCAGCAACAAAATGTACAACTTA
GTCTCACAGAGGCCACTGAGAAAGCAAAACAAGAATTTGAAAAAGCAGGGAAGGATTTCC
TGGTAGAGACTATAAAATTGGGAAAATTACTTCGGCCAAATCACTTGTGGGGTTATTATC
TTTTTCCGGATTGTTACAACCATCACTATAAGAAACCCGGTTACAATGGAAGTTGCTTCA
ATGTAGAAATAAAAAGAAATGATGATCTCAGCTGGTTGTGGAATGAAAGCACTGCTCTTT
ACCCATCCATTTATTTGAACACTCAGCAGTCTCCTGTAGCTGCTACACTCTATGTGCGCA
ATCGAGTTCGGGAAGCCATCAGAGTTTCCAAAATACCTGATGCAAAAAGTCCACTTCCGG
TTTTTGCATATACCCGCATAGTTTTTACTGATCAAGTTTTGAAATTCCTTTCTCAAGATG
AACTTGTGTATACATTTGGCGAAACTGTTGCTCTGGGTGCTTCTGGAATTGTAATATGGG
GAACCCTCAGTATAATGCGAAGTATGAAATCTTGCTTGCTCCTAGACAATTACATGGAGA
CTATACTGAATCCTTACATAATCAACGTCACACTAGCAGCCAAAATGTGTAGCCAAGTGC
TTTGCCAGGAGCAAGGAGTGTGTATAAGGAAAAACTGGAATTCAAGTGACTATCTTCACC
TCAACCCAGATAATTTTGCTATTCAACTTGAGAAAGGTGGAAAGTTCACAGTACGTGGAA
AACCGACACTTGAAGACCTGGAGCAATTTTCTGAAAAATTTTATTGCAGCTGTTATAGCA
CCTTGAGTTGTAAGGAGAAAGCTGATGTAAAAGACACTGATGCTGTTGATGTGTGTATTG
CTGATGGTGTCTGTATAGATGCTTTTCTAAAACCTCCCATGGAGACAGAAGAACCTCAAA
TTTTCTACAATGCTTCACCCTCCACACTATCTGCCACAATGTTCATTTGGAGGCTGGAAG
TCTGGGATCAAGGTATTAGCAGAATTGGTTTCTTCTGAGAGTCATGAGGGAAAAATGTGT
TTCAGGCCTCTTCCCTTGGCTTACAGGAAATGAAAAAACCATGACTATCATCACCAACAT
CCTTGGGTATTAAGTGCAGTCACTCTCCTAGATGCTGTGGGGAGAAGGCAAGTTACAAAG
ATAGACCTTCCCTCAAGATAATCAGATTTTCATGGTATTATCCTTAACCTTTTTGACATC
ATGGAGGCTTTGGGAATCTGATGAAGCCTATCAATTTTCTTCCAGAAGATATTTATATAA
GATTATAAGAAAAATTATGTACACAGCTTATTTTATTGCATTGGATCAAAATGCCATTTA
TAAAGAATTATGCCTTTTCCATCAATTTTAGCATGGAAAAATAATTTCAGGCAATATGCT
TAAAAATTGGGGGAAGACAAAAGAAATCCATATCGTGTAAATAAAAATAAATTTTGGTTT
TGCTCAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_003117 unedited
TCTGATATTTGTAATACGACTTCTTATAGGGCGGCCGCATAACTTCGTATAGCATACATT
ATACTAATTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGAGGTGG
TTAGCAGCACCTCATAAGGTCCTTCCTAGCAAGGGATGCTAATGACTAGCCAATGCTCTA
GGAAGACATTGAGACCAGCCAACTTCTTGCCTTGATAACTACTGAAGAGACATTGGGTGG
CTGGATTTTGAAAGCAGACTTCTGGTTATAGGTGATGCAACTTGAAAAACAATCCTGAAA
CATGAAACAAGAATAATAATATTTAAATGTAACTTAATCATTATACCTCTTTATCCATCA
AAGTGAATTCATTCCATTCCCTTTCATCTGTGCTCATACTTTGCATCAGATATTGGGTAA
ACCAAAGTGTGTAGGAAGAAATAAATGTTTTCATAGTCATTACTCTTTACAATGGGAGTG
CTAAAATTCAAGCACATCTTTTTCAGAAGCTTTGTTAAATCAAGTGGAGTATCCCAGATA
GTTTTCACCTTCCTTCTGATTCCATGTTGCTTGACTCTGAATTTCAGAGCACCTCCTGTT
ATTCCAAATGTGCCTTTCCTCTGGGCCTGGAATGCCCCAAGTGAATTNTGTCTTGGAAAA
TTTGATGAGCCACTAGATATGAGCCTCTTCTCTTTCATAGGAAGCCCCCGAATAAACGCC
ACCGGGCAAGGGTGTTACAATATTTTATGTTGATAGACTTGGCTACTATCCTTACATAGA
TTCAATCACAGGAGTAACTGTGAATGGAGGAATCCNCCAGAAGATTTCCTTNACAGACCA
TCTGGACAAGCTAAGAAAGACATACATTTTATATGCCAGTAGACATTTTGGAATGGGCTG
TATGACTGGGAAGATGGAGACCCACTTGGGCAGAACTGGAAACTAAAGAGT
Restriction Sites Please inquire     
ACCN NM_003117
Insert Size 2400 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003117.3, NP_003108.2
RefSeq Size 2395 bp
RefSeq ORF 1536 bp
Locus ID 6677
Cytogenetics 7q31.32
Protein Families Druggable Genome, Transmembrane
Protein Pathways Glycosaminoglycan degradation, Metabolic pathways
Gene Summary 'Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.