ALS (IGFALS) (NM_004970) Human Untagged Clone

CAT#: SC122694

IGFALS (untagged)-Human insulin-like growth factor binding protein, acid labile subunit (IGFALS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGFALS
• Synonyms: ACLSD; ALS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004970 edited
  GGAAATCCAGAGGGCAGGGGTGGCCGGCACAGCAGACGTACCCTCCCTCGCTGCCTGCCT
  GCAGCCTGCCCTGCATGCAGGATGGCCCTGAGGAAAGGAGGCCTGGCCCTGGCGCTGCTG
  CTGCTGTCCTGGGTGGCACTGGGCCCCCGCAGCCTGGAGGGAGCAGACCCCGGAACGCCG
  GGGGAAGCCGAGGGCCCAGCGTGCCCGGCCGCCTGTGTCTGCAGCTACGATGACGACGCG
  GATGAGCTCAGCGTCTTCTGCAGCTCCAGGAACCTCACGCGCCTGCCTGATGGAGTCCCG
  GGCGGCACCCAAGCCCTGTGGCTGGACGGCAACAACCTCTCGTCCGTCCCCCCGGCAGCC
  TTCCAGAACCTCTCCAGCCTGGGCTTCCTCAACCTGCAGGGCGGCCAGCTGGGCAGCCTG
  GAGCCACAGGCGCTGCTGGGCCTAGAGAACCTGTGCCACCTGCACCTGGAGCGGAACCAG
  CTGCGCAGCCTGGCACTCGGCACGTTTGCACACACGCCCGCGCTGGCCTCGCTCGGCCTC
  AGCAACAACCGTCTGAGCAGGCTGGAGGACGGGCTCTTCGAGGGCCTCGGCAGCCTCTGG
  GACCTCAACCTCGGCTGGAATAGCCTGGCGGTGCTCCCCGATGCGGCGTTCCGCGGCCTG
  GGCAGCCTGCGCGAGCTGGTGCTGGCGGGCAACAGGCTGGCCTACCTGCAGCCCGCGCTC
  TTCAGCGGCCTGGCCGAGCTCCGGGAGCTGGACCTGAGCAGGAACGCGCTGCGGGCCATC
  AAGGCAAACGTGTTCGTGCAGCTGCCCCGGCTCCAGAAACTCTACCTGGACCGCAACCTC
  ATCGCTGCCGTGGCCCCGGGCGCCTTCCTGGGCCTGAAGGCGCTGCGATGGCTGGACCTG
  TCCCACAACCGCGTGGCTGGCCTCCTGGAGGACACGTTCCCCGGTCTGCTGGGCCTGCGT
  GTGCTGCGGCTGTCCCACAACGCCATCGCCAGCCTGCGGCCCCGCACCTTCAAGGACCTG
  CACTTCCTGGAGGAGCTGCAGCTGGGCCACAACCGCATCCGGCAGCTGGCTGAGCGCAGC
  TTTGAGGGCCTGGGGCAGCTTGAGGTGCTCACGCTAGACCACAACCAGCTCCAGGAGGTC
  AAGGCGGGCGCTTTCCTCGGCCTCACCAACATGGCGGTCATGAACCTCTCTGGGAACTGT
  CTCCGGAACCTTCCGGAGCAGGTGTTCCGGGGCCTGGGCAAGCTGCACAGCCTGCACCTG
  GAGGGCAGCTGCCTGGGACGCATCCGCCCGCACACCTTCACCGGCCTCTCGGGGCTCCGC
  CGACTCTTCCTCAAGGACAACGGCCTCGTGGGCATTGAGGAGCAGAGCCTGTGGGGGCTG
  GCGGAGCTGCTGGAGCTCGACCTGACCTCCAACCAGCTCACGCACCTGCCCCACCGCCTC
  TTCCAGGGCCTGGGCAAGCTGGAGTACCTGCTGCTCTCCCGCAACCGCCTGGCAGAGCTG
  CCGGCGGACGCCCTGGGCCCCCTGCAGCGGGCCTTCTGGCTGGACGTCTCGCACAACCGC
  CTGGAGGCATTGCCCAACAGCCTCTTGGCACCACTGGGGCGGCTGCGCTACCTCAGCCTC
  AGGAACAACTCACTGCGGACCTTCACGCCGCAGCCCCCGGGCCTGGAGCGCCTGTGGCTG
  GAGGGTAACCCCTGGGACTGTGGCTGCCCTCTCAAGGCGCTGCGGGACTTCGCCCTGCAG
  AACCCCAGTGCTGTGCCCCGCTTCGTCCAGGCCATCTGTGAGGGGGACGATTGCCAGCCG
  CCCGCGTACACCTACAACAACATCACCTGTGCCAGCCCGCCCGAGGTCGTGGGGCTCGAC
  CTGCGGGACCTCAGCGAGGCCCACTTTGCTCCCTGCTGACCAGGTCCCCGGACTCAAGCC
  CCGGACTCAGGCCCCCACCTGGCTCACCTTGTGCTGGGGACAGGTCCTCAGTGTCCTCAG
  GGGCCTGCCCAGTGCACTTGCTGGAAGACGCAAGGGCCTGATGGGGTGGAAGGCATGGCG
  GCCCCCCCAGCTGTCATCAATTAAAGGCAAAGGCAATCGAATCTAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004970 unedited
  GCGNAATTCCCGGGTATGGAAATCCAGAGTGCAGGGGTGGCCGGCACAGCAGACGTACCC
  TCCCTCGCTGCCTGCCTGCAGCCTGCCCTGCATGCAGGATGGCCCTGAGGAAAGGAGGCC
  TGGCCCTGGCGCTGCTGCTGCTGTCCTGGGTGGCACTGGGCCCCCGCAGCCTGGAGGGAG
  CAGACCCCGGAACGCCGGGGGAAGCCGAGGGCCCAGCGTGCCCGGCCTCCTGTGTCTGCA
  GCTACGATGACGACGCGGATGAGCTCAGCGTCTTCTGCAGCTCCAGGAACCTCACGCGCC
  TGCCTGATGGAGTCCCGGGCGGCACCCAAGCCCTGTGGCTGGACGGCAACAACCTCTCGT
  CCGTCCCCCCGGCAGCCTTCCAGAACCTCTCCAGCCTGGGCTTCCTCAACCTGCAGGGCG
  GCCAGCTGGGCAGCCTGGAGCCACAGGCGCTGCTGGGCCTAGAGAACCTGTGCCACCTGC
  ACCTGGAGCGGAACCAGCTGCGCAGCCTGGCACTCGGCACGTTTGCACACACGCCCGCGC
  TGGCCTCGCTCGGCCTCAGCAACAACCGTCTGAGCAGGCTGGAGGACGGGCTCTTCGAGG
  GCCTCGGCAGCCTCTGGGACCTCAACCTCGGCTGGAATAGCCTGGCGGTGCTCCCCGATG
  CGGCGTTCCGCGGCCTGGGCAGCCTGCGCGAGCTGGTGCTGGCGGGCAACAGGCTGGCCT
  ACCTGCAGCCCGCGCTCTTCAGCGGCCTGGCCGAGCTCCGGGAGCTGGACCTGAGCCAGA
  ACGCGCTGCGGGCCATCAAGGCAAACGTGTTCGTGCAGCTGCCCCGGCTCCAGAAACTCT
  ACCTGGACCGCAACCTCATCGCTGCCGTGGGCCCCGG
  
• Restriction Sites: Please inquire
• ACCN: NM_004970
• Insert Size: 2168 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004970.1, NP_004961.1
• RefSeq Size: 2125 bp
• RefSeq ORF: 1818 bp
• Locus ID: 3483
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.