EYA2 (NM_005244) Human Untagged Clone

CAT#: SC122703

EYA2 (untagged)-Human eyes absent homolog 2 (Drosophila) (EYA2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EYA2
• Synonyms: EAB1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005244 edited
  GGCACGAGGGCGCGCTCTGCTCGGCTGCAGGCTCAGGAAGCGGCGCGGCGGCAGCTGCTG
  GGCCAGAGCCCCGCGCCCTGCGTCCAGCCGCCGGGTGTGTGCGCCCGCGTAGCCCAGCGT
  CGCGCCTCGGCGCCCCCACGCGCCCACGCAGCGGCGCGGGGACCCGGGCGGGGGCAGAGG
  GAGGGCCCGGCCCAGGGAGGAGAAGGGGCCGGTCCTCCCGGCGCAGGCAGCAGCCGCGGC
  AGCCCAGGAGGCGGAGGCAGCGGCAACGGCAGAGACAGCAACGTGCCCGCCGCAGTCAGC
  CCGGCCTCGTCGGACCCGCACCGGCCCGCCCGCCCGCCCGCACCGCGTCGGGGCGCCCTC
  TCCACTGCGCGCGGTACAAGGAAATGGTAGAACTAGTGATCTCACCCAGCCTCACTGTAA
  ACAGCGATTGTCTGGATAAACTGAAGTTTAACCGTGCTGACGCTGCTGTGTGGACTCTGA
  GTGACAGACAAGGCATCACCAAATCGGCCCCCCTGAGAGTGTCCCAGCTCTTCTCCAGAT
  CTTGCCCACGTGTCCTCCCCCGCCAGCCTTCCACAGCCATGGCAGCCTACGGCCAGACGC
  AGTACAGTGCGGGGATCCAGCAGGCTACCCCCTATACAGCTTACCCACCTCCAGCACAAG
  CCTATGGAATCCCTTCCTACAGCATCAAGACAGAAGACAGCTTGAACCATTCCCCTGGCC
  AGAGTGGATTCCTCAGCTATGGCTCCAGCTTCAGCACCTCACCCACTGGACAGAGCCCAT
  ACACCTACCAGATGCACGGCACAACAGGGTTCTATCAAGGAGGAAATGGACTGGGCAACG
  CAGCCGGTTTCGGGAGTGTGCACCAGGACTATCCTTCCTACCCCGGCTTCCCCCAGAGCC
  AGTACCCCCAGTATTACGGCTCATCCTACAACCCTCCCTACGTCCCGGCCAGCAGCATCT
  GCCCTTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCATCTCACAACGTCCCCA
  ACCAGAGTTCCGAGTCACTTGCTGGTGAATACAACACACACAATGGACCTTCCACACCAG
  CGAAAGAGGGAGACACAGACAGGCCGCACCGGGCCTCCGACGGGAAGCTCCGAGGCCGGT
  CTAAGAGGAGCAGTGACCCGTCCCCGGCAGGGGACAATGAGATTGAGCGTGTGTTCGTGT
  GGGACTTGGATGAGACAATAATTATTTTTCACTCCTTACTCACGGGGACATTTGCATCCA
  GATACGGGAAGGACACCACGACGTCCGTGCGCATTGGCCTTATGATGGAAGAGATGATCT
  TCAACCTTGCAGATACACATCTGTTCTTCAATGACCTGGAGGATTGTGACCAGATCCACG
  TTGATGACGTCTCATCAGATGACAATGGCCAAGATTTAAGCACATACAACTTCTCCGCTG
  ACGGCTTCCACAGTTCGGCCCCAGGAGCCAACCTGTGCCTGGGCTCTGGCGTGCACGGCG
  GCGTGGACTGGATGAGGAAGCTGGCCTTCCGCTACCGGCGGGTGAAGGAGATGTACAATA
  CCTACAAGAACAACGTTGGTGGGAAGGAGAGCTGCTTCGAGAGGATAATGCAGAGATTCG
  GCAGAAAAGCTGTCTACGTGGTGATCGGTGATGGTGTGGAAGAGGAGCAAGGAGCGAAAA
  AGCACAACATGCCTTTCTGGCGGATATCCTGCCACGCAGACCTGGAGGCACTGAGGCACG
  CCCTGGAGCTGGAGTATTTATAGCAGGATCAGCAGCATCTCCACCTGCCATCTCACCCTC
  AGACCCCCTCGCCTTCCCCACCTCCCCACCGAGAACTCCAGAGACCCAGATGTTGGACAC
  CAGGAAGGGGCCCCACAGCCGAGACGACGTGTCCAGTGACCATCTCAGAAGCCGTCCATC
  AGTCCAAATGGGGGTTCTGAGAAGGAAAGTACCCAACATTGGCTTCGGAGTATTTGACTT
  TGGGGAAAAGGGCTGGCTCGGAGTCTAGACTCTTCTGTAAGACTCACAGAACAAAAGCAA
  GGAATTGCTGATTTGGGGGGTGCCTGGTGATGAGGAGGGGATGGGTTTGTCTTGTCTTCT
  TTTTAATTTATGGACTAGTCTCATTACTCCGGAATTATGCTCTTGTACCTGTGTGGCTGG
  GTTTCTTAGTCGTTGGTTTGGTTTGGTTTTTTGAACTGGTATGTGGGGTGGTTCACAGTT
  CTAATGTAAGCACTCTATTCTCCAAGTTGTGCTTTGTGGGGACAATCATTCTTTGAACAT
  TAGAGAGGAAGGCAGTTCAAGCTGTTGAAAAGACTATTGCTTATTTTTGTTTTTAAAGAC
  CTACTTGACGTCATGTGGACAGTGCACGTGCCTTACGCTACATCTTGTTTTCTAGGAAGA
  GGGGGATGCTGGGAAGGAATGGGTGCTTTGTGATGGATAAAAGGCATTAAATAAAACCAC
  GTTTACATTTTGAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005244 unedited
  NCATTACGNCATTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGGGGCGC
  GCTCTGCTCGGCTGCAGGCTCATGAAGCGGCGCGGCGGCAGCTGCTGGGCCAGAGCCCCG
  CGCCCTGCGTCCAGCCGCCGGGTGTGTGCGCCCGCGTAGCCCATCGTCGCGCCTCGGCGC
  CCCCACGCGCCCACGCAGCGGCGCGGGGACCCGGGCGGGGGCAGAGGGAGGGCCCGGCCC
  AGGGAGGAGAAGGGGCCGGTCCTCCCGGCGCAGGCAGCAGCCGCGGCAGCCCATGAGGCG
  GAGGCAGCGGCAACGGCAGAGACAGCAACGTGCCCGCCGCAGTCAGCCCGGCCTCGTCGG
  ACCCGCACCGGCCCGCCCGCCCGCCCGCACCGCGTCGGGGCGCCCTCTCCACTGCGCGCG
  GTACAAGGAAATGGTAGAACTAGTGATCTCACCCAGCCTCACTGTAAACAGCGATTGTCT
  GGATAAACTGAAGTTTAACCGTGCTGACGCTGCTGTGTGGACTCTGAGTGACAGACAAGG
  CATCACCAAATCGGCCCCCCTGAGAGTGTCCCAGCTCTTCTCCAGATCTTGCCCACGTGT
  CCTCCCCCGCCAGCCTTCCACAGCCATGGCAGCCTACGGCCAGACGCAGTACAGTGCGGT
  GATCCAGCAGGCTACCCNCTATACAGCTTACCCACCTCCAGCACAAGCCTATGGAATCCC
  TTCCTACAGCATCAAGACAGAAGACAGCTTGGACCATTCCCCTGGCCAGAGTGGATCCCT
  CATCTATGGCTCCAGCTTCAGCACCTCACCCATGGACAGAGCCCATACACCTACCAGATG
  CACGGNACANCAGGGTTCTATCATGGAGGGAAATGGACTGGNCAACGCAACCGGTTT
  
• Restriction Sites: Please inquire
• ACCN: NM_005244
• Insert Size: 2492 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005244.3, NP_005235.3
• RefSeq Size: 2478 bp
• RefSeq ORF: 1617 bp
• Locus ID: 2139
• Cytogenetics: 20q13.12
• Protein Families: Phosphatase, Transcription Factors
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Alternative splicing results in multiple transcript variants, but the full-length natures of all of these variants have not yet been determined. [provided by RefSeq, Jul 2009]'
  Transcript Variant: This variant (1), also known as EYA2I, represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.