Alanine Transaminase (GPT) (NM_005309) Human Untagged Clone

CAT#: SC122706

GPT (untagged)-Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPT
• Synonyms: AAT1; ALT1; GPT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005309 edited
  AGGGCTGCCACTTGGACTCCCTTGCCAGGGGGCGGGCAGGGGCTGGCTCTGCCTGGGGCC
  AGGCCCATGTTAACCTGTGCCCCGGCCTGGGGGCCAGAGGGGCAGGCCTGCGCACGGGGA
  GGCTGTTGTGGCAGCGGAACAGAGACGTGCACGAGTCATGGCCTCGAGCACAGGTGACCG
  GAGCCAGGCGGTGAGGCATGGACTGAGGGCGAAGGTGCTGACGCTGGACGGCATGAACCC
  GCGTGTGCGGAGAGTGGAGTACGCAGTGCGTGGCCCCATAGTGCAGCGAGCCTTGGAGCT
  GGAGCAGGAGCTGCGCCAGGGTGTGAAGAAGCCTTTCACCGAGGTCATCCGTGCCAACAT
  CGGGGACGCACAGGCTATGGGGCAGAGGCCCATCACCTTCCTGCGCCAGGTCTTGGCCCT
  CTGTGTTAACCCTGATCTTCTGAGCAGCCCCAACTTCCCTGACGATGCCAAGAAAAGGGC
  GGAGCGCATCTTGCAGGCGTGTGGGGGCCACAGTCTGGGGGCCTACAGCGTCAGCTCCGG
  CATCCAGCTGATCCGGGAGGACGTGGCGCGGTACATTGAGAGGCGTGACGGAGGCATCCC
  TGCGGACCCCAACAACGTCTTCCTGTCCACAGGGGCCAGCGATGCCATCGTGACGGTGCT
  GAAGCTGCTGGTGGCCGGCGAGGGCCACACACGCACGGGTGTGCTCATCCCCATCCCCCA
  GTACCCACTCTACTCGGCCACGCTGGCAGAGCTGGGCGCAGTGCAGGTGGATTACTACCT
  GGACGAGGAGCGTGCCTGGGCGCTGGACGTGGCCGAGCTTCACCGTGCACTGGGCCAGGC
  GCGTGACCACTGCCGCCCTCGTGCGCTCTGTGTCATCAACCCTGGCAACCCCACCGGGCA
  GGTGCAGACCCGCGAGTGCATCGAGGCCGTGATCCGCTTCGCCTTCGAAGAGCGGCTCTT
  TCTGCTGGCGGACGAGGTGTACCAGGACAACGTGTACGCCGCGGGTTCGCAGTTCCACTC
  ATTCAAGAAGGTGCTCATGGAGATGGGGCCGCCCTACGCCGGGCAGCAGGAGCTTGCCTC
  CTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGCGGGTTCCGCGGCGGCTATGTGGA
  GGTGGTGAACATGGACGCTGCAGTGCAGCAGCAGATGCTGAAGCTGATGAGTGTGCGGCT
  GTGCCCGCCGGTGCCAGGACAGGCCCTGCTGGACCTGGTGGTCAGCCCGCCCGCGCCCAC
  CGACCCCTCCTTTGCGCAGTTCCAGGCTGAGAAGCAGGCAGTGCTGGCAGAGCTGGCGGC
  CAAGGCCAAGCTCACCGAGCAGGTCTTCAATGAGGCTCCTGGCATCAGCTGCAACCCAGT
  GCAGGGCGCCATGTACTCCTTCCCGCGCGTGCAGCTGCCCCCGCGGGCGGTGGAGCGCGC
  TCAGGAGCTGGGCCTGGCCCCCGATATGTTCTTCTGCCTGCGCCTCCTGGAGGAGACCGG
  CATCTGCGTGGTGCCAGGGAGCGGCTTTGGGCAGCGGGAAGGCACCTACCACTTCCGGAT
  GACCATTCTGCCCCCCTTGGAGAAACTGCGGCTGCTGCTGGAGAAGCTGAGCAGGTTCCA
  TGCCAAGTTCACCCTCGAGTACTCCTGAGCACCCCAGCTGGGGCCAGGCTGGGTCGCCCT
  GGACTGTGTGCTCAGGAGCCCTGGGAGGCTCTGGAGCCCACTGTACTTGCTCTTGATGCC
  TGGCGGGGTGGGGTGGGGGGGGTGCTGGGCCCCTGCCTCTCTGCAGGTCCCTAATAAAGC
  TGTGTGGCAGTCTGACTCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005309 unedited
  GGTCAGAATTTTGTATACGACTCATATAGGGCGGCCGCGATTCCCGGGATATCGTCGACC
  CACGCGTCCGAGGGCTGCCACTTGGACTCCCTTGCCAGGGGGCGGGCAGGGGCTGGCTCT
  GCCTGGGGCCAGGCCCATGTTAACCTGTGCCCCGGCCTGGGGGCCAGAGGGGCAGGCCTG
  CGCACGGGGAGGCTGTTGTGGCAGCGGAACAGAGACGTGCACGAGTCATGGCCTCGAGCA
  CAGGTGACCGGAGCCAGGCGGTGAGGCATGGACTGAGGGCGAAGGTGCTGACGCTGGACG
  GCATGAACCCGCGTGTGCGGAGAGTGGAGTACGCAGTGCGTGGCCCCATAGTGCAGCGAG
  CCTTGGAGCTGGAGCAGGAGCTGCGCCAGGGTGTGAAGAAGCCTTTCACCGAGGTCATCC
  GTGCCAACATCGGGGACGCACAGGCTATGGGGCAGAGGCCCATCACCTTCCTGCGCCAGG
  TCTTGGCCCTCTGTGTTAACCCTGATCTTCTGAGCAGCCCCAACTTCCCTGACGATGCCA
  AGAAAAGGGCGGAGCGCATCTTGCAGGCGTGTGGGGGCCACAGTCTGGGGGCCTACAGCG
  TCAGCTCCGGCATCCAGCTGATCCGGGAGGACGTGGCGCGGTACATTGAGAGGCGTGACG
  GAGGCATCCCTGCGGACCCCACAACGTCTTCCTGTCCACAGGGGCCAGCGATGCCATCGT
  GACGGTGCTGAAGCTGCTGGTGGCCGGCGAGGGCCACACACGCCCGGGTGTGCTCATCCC
  CATCCCCCAGTACCCACTCTACTCGGCCACGCTGGCAGAGCTGGGCGCAGTGCAGGTGGA
  TTACTACCTGGACAAGGAGCGTGCCTGGGCGCCTGGACTTGGCCGAACTTACCGTGCACT
  GGGCCAGGCGCGTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_005309
• ORF Size: 1491 bp
• Insert Size: 1871
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_005309.1, NP_005300.1
• RefSeq Size: 1924
• RefSeq ORF: 1491
• Locus ID: 2875
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways
• Gene Summary: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase. [provided by RefSeq, Nov 2009]