Smoothened (SMO) (NM_005631) Human Untagged Clone

CAT#: SC122724

SMO (untagged)-Human smoothened, frizzled family receptor (SMO)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMO
• Synonyms: CRJS; FZD11; Gx; SMOH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005631 edited
  GGGCTGAAGACAACTTGGATTGCGAGGCTAGGGCTTGGGGAGTCGTGCATCCCGTTCCGG
  GCCTCCGCAGCCCAACATGGGCCCCGGGTTCCAAAGTTTGCGAAGTTGGGCGCCGAGGGG
  CCGGGGCGCGCGGAGCGTCCGGGGGGGCCCGGGCCCGGATTCTCTGGGCGCACAGGTCGC
  CTGAGCCGCCTCCGCGGCCGCCGAGGTCGTGCGTGTGGCCGGGGGGCTCCGAGGAGCAGG
  CGGGGGCGCCGGGGCTTTTGCTGAGTTGGCGGGGTTGGCCATGGCCGCTGCCCGCCCAGC
  GCGGGGGCCGGAGCTCCCGCTCCTGGGGCTGCTGCTGCTGCTGCTGCTGGGGGACCCGGG
  CCGGGGGGCGGCCTCGAGCGGGAACGCGACCGGGCCTGGGCCTCGGAGCGCGGGCGGGAG
  CGCGAGGAGGAGCGCGGCGGTGACTGGCCCTCCGCCGCCGCTGAGCCACTGCGGCCGGGC
  TGCCCCCTGCGAGCCGCTGCGCTACAACGTGTGCCTGGGCTCGGTGCTGCCCTACGGGGC
  CACCTCCACACTGCTGGCCGGAGACTCGGACTCCCAGGAGGAAGCGCACGGCAAGCTCGT
  GCTCTGGTCGGGCCTCCGGAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTG
  TGCCGTATACATGCCCAAGTGTGAGAATGACCGGGTGGAGCTGCCCAGCCGTACCCTCTG
  CCAGGCCACCCGAGGCCCCTGTGCCATCGTGGAGAGGGAGCGGGGCTGGCCTGACTTCCT
  GCGCTGCACTCCTGACCGCTTCCCTGAAGGCTGCACGAATGAGGTGCAGAACATCAAGTT
  CAACAGTTCAGGCCAGTGCGAAGTGCCCTTGGTTCGGACAGACAACCCCAAGAGCTGGTA
  CGAGGACGTGGAGGGCTGCGGCATCCAGTGCCAGAACCCGCTCTTCACAGAGGCTGAGCA
  CCAGGACATGCACAGCTACATCGCGGCCTTCGGGGCCGTCACGGGCCTCTGCACGCTCTT
  CACCCTGGCCACATTCGTGGCTGACTGGCGGAACTCGAATCGCTACCCTGCTGTTATTCT
  CTTCTACGTCAATGCGTGCTTCTTTGTGGGCAGCATTGGCTGGCTGGCCCAGTTCATGGA
  TGGTGCCCGCCGAGAGATCGTCTGCCGTGCAGATGGCACCATGAGGCTTGGGGAGCCCAC
  CTCCAATGAGACTCTGTCCTGCGTCATCATCTTTGTCATCGTGTACTACGCCCTGATGGC
  TGGTGTGGTTTGGTTTGTGGTCCTCACCTATGCCTGGCACACTTCCTTCAAAGCCCTGGG
  CACCACCTACCAGCCTCTCTCGGGCAAGACCTCCTACTTCCACCTGCTCACCTGGTCACT
  CCCCTTTGTCCTCACTGTGGCAATCCTTGCTGTGGCGCAGGTGGATGGGGACTCTGTGAG
  TGGCATTTGTTTTGTGGGCTACAAGAACTACCGATACCGTGCGGGCTTCGTGCTGGCCCC
  AATCGGCCTGGTGCTCATCGTGGGAGGCTACTTCCTCATCCGAGGAGTCATGACTCTGTT
  CTCCATCAAGAGCAACCACCCCGGGCTGCTGAGTGAGAAGGCTGCCAGCAAGATCAACGA
  GACCATGCTGCGCCTGGGCATTTTTGGCTTCCTGGCCTTTGGCTTTGTGCTCATTACCTT
  CAGCTGCCACTTCTACGACTTCTTCAACCAGGCTGAGTGGGAGCGCAGCTTCCGGGACTA
  TGTGCTATGTCAGGCCAATGTGACCATCGGGCTGCCCACCAAGCAGCCCATCCCTGACTG
  TGAGATCAAGAATCGCCCGAGCCTTCTGGTGGAGAAGATCAACCTGTTTGCCATGTTTGG
  AACTGGCATCGCCATGAGCACCTGGGTCTGGACCAAGGCCACGCTGCTCATCTGGAGGCG
  TACCTGGTGCAGGTTGACTGGGCAGAGTGACGATGAGCCAAAGCGGATCAAGAAGAGCAA
  GATGATTGCCAAGGCCTTCTCTAAGCGGCACGAGCTCCTGCAGAACCCAGGCCAGGAGCT
  GTCCTTCAGCATGCACACTGTGTCCCACGACGGGCCCGTGGCGGGCTTGGCCTTTGACCT
  CAATGAGCCCTCAGCTGATGTCTCCTCTGCCTGGGCCCAGCATGTCACCAAGATGGTGGC
  TCGGAGAGGAGCCATACTGCCCCAGGATATTTCTGTCACCCCTGTGGCAACTCCAGTGCC
  CCCAGAGGAACAAGCCAACCTGTGGCTGGTTGAGGCAGAGATCTCCCCAGAGCTGCAGAA
  GCGCCTGGGCCGGAAGAAGAAGAGGAGGAAGAGGAAGAAGGAGGTGTGCCCGCTGGCGCC
  GCCCCCTGAGCTTCACCCCCCTGCCCCTGCCCCCAGTACCATTCCTCGACTGCCTCAGCT
  GCCCCGGCAGAAATGCCTGGTGGCTGCAGGTGCCTGGGGAGCTGGGGACTCTTGCCGACA
  GGGAGCGTGGACCCTGGTCTCCAACCCATTCTGCCCAGAGCCCAGTCCCCCTCAGGATCC
  ATTTCTGCCCAGTGCACCGGCCCCCGTGGCATGGGCTCATGGCCGCCGACAGGGCCTGGG
  GCCTATTCACTCCCGCACCAACCTGATGGACACAGAACTCATGGATGCAGACTCGGACTT
  CTGAGCCTGCAGAGCAGGACCTGGGACAGGAAAGAGAGGAACCAATACCTTCAAGGCTCT
  TCTTCCTCACCGAGCATGCTTCCCTAGGATCCCGTCTTCCAGAGAACCTGTGGGCTGACT
  GCCCTCCGAAGAGAGTTCTGGATGTCTGGCTCAAAGCAGCAGGACTGTGGGAAAGAGCCT
  AACATCTCCATGGGGAGGCCTCACCCCAGGGACAGGGCCCTGGAGCTCAGGGTCCTTGTT
  TCTGCCCTGCCAGCTGCAGCCTGGTTGGCAGCATCTGCTCCATCGGGGCAGGGGGTATGC
  AGAGCTTGTGGTGGGGCAGGAACGGTGGAGGCAGAGGTGACAGTTCCCAGAGTGGGCTTT
  GGTGGCCAGGGAGGCAGCCTAGCCTATGTCTGGCAGATGAGGGCTGGCTGCCGTTTTCTG
  GGCTGATGGGTGCCCTTTCCTGGCAGTCTCAGTCCAAAAGTGTTGACTGTGTCATTAGTC
  CTTTGTCTAAGTAGGGCCAGGGCACCGTATTCCTCTCCCAGGTGTTTGTGGGGCTGGAAG
  GACCTGCTCCCACAGGGGCCATGTCCTCTCTTAATAGGTGGCACTACCCCAAACCCATCT
  TTTGTTCTCCTATATCCTCCTTCTCCTGTTCCATTTCAGTTCAGTTTCAGCGGTGCCAAC
  CTCTTTGCGTTTCCTTTTTGTTGATGAGGACCCAGAGCTGCTGCACACACTCACCTCTAA
  CCCCCTCCCCTCGCTGCTGGGCCCCATCTCCACAGGAGAGACTGGTTCGGCTCTAGGGCC
  TCAGTCTGGAGTGGGATAGGAGCAGTGAGTGACAAAGCCTCTGAAAGATGCATCATCTCT
  TCCTCACACCCATTTAGTGGGGGATGGGTCCTCTAGACTTGAGGGGCTACCCTGGGAAGC
  TGCCGTAGCTTCAGCCAGGCAAGAAAGCTTCCTTCAACCTGCATAGCCGGTGGGTGAGGA
  GATTCCCACCTTCCATAGCCTCCAAACATGTTCCCAAGGCCCCACTTTCAAGAATCAGAC
  AGCAGGAAGCCATAGATGCTGGCTGGGTTCCAGGTTATGGGGAGAAGAAATACAGTCAAT
  AAAAGGTTTTTGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005631 unedited
  NNNNAAATGGCAACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAG
  GGCTGAAGACAACTTGGATTGCGAGGCTAGGGCTTGGGGAGTCGTGCATCCCGTTCCGGG
  CCTCCGCAGCCCAACATGGGCCCCGGGTTCCAAAGTTTGCGAAGTTGGGCGCCGAGGGGC
  CGGGGCGCGCGGAGCGTCCGGGGGGGCCCGGGCCCGGATTCTCTGGGCGCACAGGTCGCC
  TGAGCCGCCTCCGCGGCCGCCGAGGTCGTGCGTGTGGCCGGGGGGCTCCGAGGAGCAGGC
  GGGGGCGCCGGGGCTTTTGCTGAGTTGGCGGGGTTGGCCATGGCCGCTGCCCGCCCAGCG
  CGGGGGCCGGAGCTCCCGCTCCTGGGGCTGCTGCTGCTGCTGCTGCTGGGGGACCCGGGC
  CGGGGGGCGGCCTCGAGCGGGAACGCGACCGGGCCTGGGCCTCGGAGCGCGGGCGGGAGC
  GCGAGGAGGAGCGCGGCGGTGACTGGCCCTCCGCCGCCGCTGAGCCACTGCGGCCGGGCT
  GCCCCCTGCGAGCCGCTGCGCTACAACGTGTGCCTGGGCTCGGTGCTGCCCTACGGTGCC
  ACCTCCACACTGCTGGCCGGAGACTCGGACTCCCAGGAGGAAGCGCACGGCAAGCTCGTG
  CTCTGGTCGGGCCTCCGGAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTGT
  GCCGTATACATGCCCAAGTGTGAGAATGACCGGGTGGAGCTGCCCAGCCGTACCCTCTGC
  CATGCCACCCGATGCCCCTGTGCCATCNTGGAAAGGGAGCGGGGCTGGCCTGACTTCCTG
  CGCTGCACTCCTGACCGCTTCCCG
  
• Restriction Sites: Please inquire
• ACCN: NM_005631
• Insert Size: 3800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005631.3, NP_005622.1
• RefSeq Size: 3772 bp
• RefSeq ORF: 2364 bp
• Locus ID: 6608
• Cytogenetics: 7q32.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• Gene Summary: 'The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]'