ANP (NPPA) (NM_006172) Human Untagged Clone

CAT#: SC122740

NPPA (untagged)-Human natriuretic peptide A (NPPA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NPPA
• Synonyms: ANF; ANP; ATFB6; ATRST2; CDD; CDD-ANF; CDP; PND
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006172 edited
  GAGACAGGGACAGACGTAGGCCAAGAGAGGGGAACCAGAGAGGAACCAGAGGGGAGAGAC
  AGAGCAGCAAGCAGTGGATTGCTCCTTGACGACGCCAGCATGAGCTCCTTCTCCACCACC
  ACCGTGAGCTTCCTCCTTTTACTGGCATTCCAGCTCCTAGGTCAGACCAGAGCTAATCCC
  ATGTACAATGCCGTGTCCAACGCAGACCTGATGGATTTCAAGAATTTGCTGGACCATTTG
  GAAGAAAAGATGCCTTTAGAAGATGAGGTCGTGCCCCCACAAGTGCTCAGTGAGCCGAAT
  GAAGAAGCGGGGGCTGCTCTCAGCCCCCTCCCTGAGGTGCCTCCCTGGACCGGGGAAGTC
  AGCCCAGCCCAGAGAGATGGAGGTGCCCTCGGGCGGGGCCCCTGGGACTCCTCTGATCGA
  TCTGCCCTCCTAAAAAGCAAGCTGAGGGCGCTGCTCACTGCCCCTCGGAGCCTGCGGAGA
  TCCAGCTGCTTCGGGGGCAGGATGGACAGGATTGGAGCCCAGAGCGGACTGGGCTGTAAC
  AGCTTCCGGTACCGAAGATAACAGCCAGGGAGGACAAGCAGGGCTGGGCCTAGGGACAGA
  CTGCAAGAGGCTCCTGTCCCCTGGGGTCTCTGCTGCATTCGTGTCATCTTGTTGCCATGG
  AGTTGTGATCATCCCATCTAAGCTGCAGCTTCCTGTCAACACTTCTCACATCTTATGCTA
  ACTGTAGATAAAGTGGTTTGATGGTGACTTCCTCGCCTCTCCCACCCCATGCATTAAATT
  TTAAGGTAGAACCTCACCTGTTACTGAAAGTGGTTTGAAAGTGAATAAACTTCAGCACCA
  TGGACAGAAGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006172 unedited
  NNNAAGTTCAAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCCATTACGGCC
  GGGGGAGAACAGGNACAGACGTAGGCCAAGAGAGGGGAACCAGAGAGGAACCAGAGGGGA
  GAGACAGAGCAGCAAGCAGTGGATTGCTCCTTGACGACGCCAGCATGAGCTCCTTCTCCA
  CCACCACCGTGAGCTTCCTCCTTTTACTGGCATTCCAGCTCCTAGGTCAGACCAGAGCTA
  ATCCCATGTACAATGCCGTGTCCAACGCAGACCTGATGGATTTCAAGAATTTGCTGGACC
  ATTTGGAAGAAAAGATGCCTTTAGAAGATGAGGTCGTGCCCCCACAAGTGCTCAGTGAGC
  CGAATGAAGAAGCGGGGGCTGCTCTCAGCCCCCTCCCTGAGGTGCCTCCCTGGACCGGGG
  AAGTCAGCCCAGCCCAGAGAGATGGAGGTGCCCTCGGGCGGGGCCCCTGGGACTCCTCTG
  ATCGATCTGCCCTCCTAAAAAGCAAGCTGAGGGCGCTGCTCACTGCCCCTCGGAGCCTGC
  GGAGATCCAGCTGCTTCGGGGGCAGGATGGACAGGATTGGAGCCCAGAGCGGACTGGGCT
  GTAACAGCTTCCGGTACCGAAGATAACAGCCAGGGAGGACAAGCAGGGCTGGGCCTAGGG
  ACAGACTGCAAGAGGCTCCTGTCCCCTGGGGTCTCTGCTGCATTCGTGTCATCTTGTTGC
  CATGGAGTTGTGATCATCCCATCTAAGCTGCAGCTTCCTGTCAACACTTCTCACATCTTA
  TGCTAACTGTAGATAAAGTGGTTTGATGGTGACTTNCTCGNNCTCTCCACCCATGCATTA
  AATTTAAGGTAGAACCTCACCCTGTACTTGAAGTGGTTTTGAAGTGAATNAACTTCAGCA
  CCATGGACAGAAGAACAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_006172
• Insert Size: 900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006172.1, NP_006163.1
• RefSeq Size: 845 bp
• RefSeq ORF: 456 bp
• Locus ID: 4878
• Cytogenetics: 1p36.22
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015]'