PDF (NM_022341) Human Untagged Clone

CAT#: SC122932

PDF (untagged)-Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022341 edited
  CATGGCCCGGCTGTGGGGCGCGCTGAGTCTTCGGCCACTGTGGGCGGCCGTGCCGTGGGG
  CGGGGCGGCAGCCGTCGGTGTCCGGGCTTGCAGCTCCACGGCCGCCCCGGACGGCGTCGA
  GGGCCCGGCGCTGCGGCGCTCCTATTGGCGCCACCTGAGGCGTCTGGTGCTGGGTCCTCC
  CGAACCGCCGTTCTCGCACGTGTGCCAAGTCGGGGACCCGGTGCTGCGCGGCGTGGCGGC
  CCCGGTGGAGCGGGCGCAGCTAGGCGGGCCCGAGCTGCAGCGGCTGACGCAACGGCTGGT
  CCAGGTGATGCGGCGGCGGCGCTGCGTGGGCCTAAGCGCGCCGCAGCTGGGGGTGCCGCG
  GCAGGTGCTGGCGCTGGAGCTCCCCGAGGCGCTGTGTCGGGAGTGCCCGCCCCGCCAGCG
  CGCGCTCCGCCAAATGGAGCCCTTCCCCCTGCGCGTGTTCGTGAACCCCAGCCTGCGAGT
  GCTTGACAGCCGCCTGGTCACCTTTCCCGAGGGCTGCGAGAGCGTCGCCGGCTTCCTGGC
  CTGCGTGCCCCGCTTCCAGGCGGTGCAGATCTCAGGGCTGGACCCCAATGGAGAACAGGT
  GGTGTGGCAGGCGAGCGGGTGGGCAGCCCGCATCATCCAGCACGAGATGGACCACCTGCA
  GGGCTGCCTGTTTATTGACAAAATGGACAGCAGGACGTTCACAAACGTCTATTGGATGAA
  GGTGAATGACTAAAGCTTTGCTACTGGGGCTGAGGATTCCGGATACCAAGACGCAAACAC
  TTTCACTTTGAGCTGGGCAAATCTTACTTGGCATCAACTTGGATGGCTCGCATATGACAG
  GAAACTGGATTGCCAAGGCATGGCAGACTGAGCTGGAGGAAGATGTCAGAAATGTTTGCC
  CTGAAATCAGTTACGGACAAAATGTTGGCTACAACTTGAGGAGAAAAATCACCCCCAAAG
  GAGTGGACATTTCCTAACAATTCTGTATGGAGGAAGGTGGGGTAATTGCATTCGTCTGCA
  GTAGACACGAGTTCCTCGGACCTGTATAATCTCCCAAAGCCAAGGTTTGGTATTAATGTA
  GTCCCCAAATACCTGAAAGCTGTCTTTAAAAATGCAGGTAAGCATGTGACTGGCAAAAAA
  AAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022341 unedited
  GGGCCGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATCCCGGGATATCGTCG
  ACCCACGCGTCCGCATGGCCCGGCTGTGGGGCGCGCTGAGTCTTCGGCCACTGTGGGCGG
  CCGTGCCGTGGGGCGGGGCGGCAGCCGTCGGTGTCCGGGCTTGCAGCTCCACGGCCGCCC
  CGGACGGCGTCGAGGGCCCGGCGCTGCGGCGCTCCTATTGGCGCCACCTGAGGCGTCTGG
  TGCTGGGTCCTCCCGAACCGCCGTTCTCGCACGTGTGCCAAGTCGGGGACCCGGTGCTGC
  GCGGCGTGGCGGCCCCGGTGGAGCGGGCGCAGCTAGGCGGGCCCGAGCTGCAGCGGCTGA
  CGCAACGGCTGGTCCAGGTGATGCGGCGGCGGCGCTGCGTGGGCCTAAGCGCGCCGCAGC
  TGGGGGTGCCGCGGCAGGTGCTGGCGCTGGAGCTCCCCGAGGCGCTGTGTCGGGAGTGCC
  CGCCCCGCCAGCGCGCGCTCCGCCAAATGGAGCCCTTCCCCCTGCGCGTGTTCGTGAACC
  CCAGCCTGCGAGTGCTTGACAGCCGCCTGGTCACCTTTCCCGAGGGCTGCGAGAGCGTCG
  CCGGCTTCCTGGCCTGCGTGCCCCGCTTCCAGGCGGTGCAGATCTCAGGGCTGGACCCCA
  ATGGAGAACAGGTGGTGTGGCANGCGAGCGGGTGGGCAGCCCGCATCATCCAGCACGAGA
  TGGACCACCTGCNAGGCTGCCTGTTTATTGANCAAATGGACAGCAGGACGTTCACAAACG
  TCTATTGGATGAAGGTGAATGACTAAAGCTTTGCTACTGGGGCTNGAGATTCCGGATACC
  AAGACGCAACACTTTCACTTTGAGCTGGGCAATCTTACTTGGCATCAACTGGGATG
  
• Restriction Sites: Please inquire
• ACCN: NM_022341
• ORF Size: 732 bp
• Insert Size: 1156
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_022341.1, NP_071736.1
• RefSeq Size: 1180
• RefSeq ORF: 732
• Locus ID: 64146
• Gene Summary: Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq, Nov 2008]