Alkaline Phosphatase (ALPPL2) (NM_031313) Human Untagged Clone

CAT#: SC123000

ALPPL2 (untagged)-Human alkaline phosphatase, placental-like 2 (ALPPL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALPG
• Synonyms: ALPPL; ALPPL2; GCAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_031313 edited
  GGGATTTCCGCCTCGCCGCTCTCCGACTGCTTCCAGACATGCAGGGGCCCTGGGTGCTGC
  TCCTGCTGGGCCTGAGGCTACAGCTCTCCCTGGGCATCATCCCAGTTGAGGAGGAGAACC
  CGGACTTCTGGAACCGCCAGGCAGCCGAGGCCCTGGGTGCCGCCAAGAAGCTGCAGCCTG
  CACAGACAGCCGCCAAGAACCTCATCATCTTCCTGGGTGACGGGATGGGGGTGTCTACGG
  TGACAGCTGCCAGGATCCTAAAAGGGCAGAAGAAGGACAAACTGGGGCCTGAGACCTTCC
  TGGCCATGGACCGCTTCCCGTACGTGGCTCTGTCCAAGACATACAGTGTAGACAAGCATG
  TGCCAGACAGTGGAGCCACAGCCACGGCCTACCTGTGCGGGGTCAAGGGCAACTTCCAGA
  CCATTGGCTTGAGTGCAGCCGCCCGCTTTAACCAGTGCAACACGACACGCGGCAACGAGG
  TCATCTCCGTGATGAATCGGGCCAAGAAAGCAGGAAAGTCAGTGGGAGTGGTAACCACCA
  CACGGGTGCAGCATGCCTCGCCAGCCGGCGCCTACGCCCACACGGTGAACCGCAACTGGT
  ACTCGGATGCCGACGTGCCTGCCTCGGCCCGCCAGGAGGGGTGCCAGGACATCGCCACGC
  AGCTCATCTCCAACATGGACATTGATGTGATCCTAGGTGGAGGCCGAAAGTACATGTTTC
  CCATGGGGACCCCAGACCCTGAGTACCCAGATGACTACAGCCAAGGTGGGACCAGGCTGG
  ACGGGAAGAATCTGGTGCAGGAATGGCTGGCGAAGCGCCAGGGTGCCCGGTACGTGTGGA
  ACCGCACTGAGCTCATGCAGGCTTCCCTGGACCCGTCTGTGACCCATCTCATGGGTCTCT
  TTGAGCCTGGAGACATGAAATACGAGATCCACCGAGACTCCACACTGGACCCCTCCCTGA
  TGGAGATGACAGAGGCTGCCCTGCGCCTGCTGAGCAGGAACCCCCGCGGCTTCTTCCTCT
  TCGTGGAGGGTGGTCGCATCGACCATGGTCATCATGAAAGCAGGGCTTACCGGGCACTGA
  CTGAGACGATCATGTTCGACGACGCCATTGAGAGGGCGGGCCAGCTCACCAGCGAGGAGG
  ACACGCTGAGCCTCGTCACTGCCGACCACTCCCACGTCTTCTCCTTCGGAGGCTACCCCC
  TGCGAGGGAGCTCCATCTTCGGGCTGGCCCCTGGCAAGGCCCGGGACAGGAAGGCCTACA
  CGGTCCTCCTATACGGAAACGGTCCAGGCTATGTGCTCAAGGACGGCGCCCGGCCGGATG
  TTACCGAGAGCGAGAGCGGGAGCCCCGAGTATCGGCAGCAGTCAGCAGTGCCCCTGGACG
  GAGAGACCCACGCAGGCGAGGACGTGGCGGTGTTCGCGCGCGGCCCGCAGGCGCACCTGG
  TTCACGGCGTGCAGGAGCAGACCTTCATAGCGCACGTCATGGCCTTCGCCGCCTGCCTGG
  AGCCCTACACCGCCTGCGACCTGGCGCCCCGCGCCGGCACCACCGACGCCGCGCACCCGG
  GGCCGTCCGTGGTCCCCGCGTTGCTTCCTCTGCTGGCAGGGACCTTGCTGCTGCTGGGGA
  CGGCCACTGCTCCCTGAGTGTCCCGTCCCTGGGGCTCCTGCTTCCCCATCCCGGAGTTCC
  CCTGCTCCCCACCTCCAGTCGTCCTGCCGGACCTCCACCTGGAGCTGTCACCCCCGGAGT
  CGCCACACAGACGTCCTGCCATGGAACCTTCCCCTCCCGGTGCACCCTGGGGACCGAGCC
  CTTGACACCACGCCCTTTGCTTTATCTTGCTCTTGAAATTTTGGCCCCAACTCCAGGGAC
  TGGGGATTTGTGCCTGGCAGCTGCCTGCATTTCAGGAAAAGAGGAGGCTCAGACCATCCA
  GCCCCCGCCCATATCCTGAGGTGGATCAGGCAGGCTCTCTCCCCGGGGACATGAGGCACC
  CATACCTAGGACCCCCTGCGCCTTTTTTAGCTTCAGTCATGGCAGCACCTGAGGGACACA
  AGGACTTGGGTGCATCAGGACGCCTTGGAGAAGCGTGGCTTCCTGCCACCCTGCAACCCA
  CCCTCCCAGCCAAGGAGGCTGCTGTGGTGGGGATCCCCAGGGGGGCTTTGACACAGTCCT
  CTGCTGTCCCTCCACTGGGCTAATTCTACACCCCTGTGCCCCTCCTAGGGGCCCATGAGT
  CAGAGAGGCTTGCCCCAAGTCACAGCCACTCAGATGTTCGACGCCCCCTAAGGTCCATTC
  CAGCACCCACCTGAGTTCCGAGGAGCACCTGGGAAGCTCTGGGTGCAGGATAGCAGTCCA
  GAGTCCATGGCCCCGCCTAGGCCATCTGGGTGCTGGGCATGGATTTCTCAGCAAGGAAGA
  CTCATTACCTTCCCTCCCTGGGCCTCCATTCTTCTGGGAAACACAAAGCAATAATAAAAG
  GAAGTGTTAGACAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031313 unedited
  GGAATGCAGTACAAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGG
  GGATTTCCGCCTCGCCGCTCTCCGACTGCTTCCAGACATGCAGGGGCCCTGGGTGCTGCT
  CCTGCTGGGCCTGAGGCTACAGCTCTCCCTGGGCATCATCCCAGTTGAGGAGGAGAACCC
  GGACTTCTGGAACCGCCAGGCAGCCGAGGCCCTGGGTGCCGCCAAGAAGCTGCAGCCTGC
  ACAGACAGCCGCCAAGAACCTCATCATCTTCCTGGGTGACGGGATGGGGGTGTCTACGGT
  GACAGCTGCCAGGATCCTAAAAGGGCAGAAGAAGGACAAACTGGGGCCTGAGACCTTCCT
  GGCCATGGACCGCTTCCCGTACGTGGCTCTGTCCAAGACATACAGTGTAGACAAGCATGT
  GCCAGACAGTGGAGCCACAGCCACGGCCTACCTGTGCGGGGTCAAGGGCAACTTCCAGAC
  CATTGGCTTGAGTGCAGCCGCCCGCTTTAACCAGTGCAACACGACACGCGGCAACGAGGT
  CATCTCCGTGATGAATCGGGCCAAGAAAGCAGGAAAGTCAGTGGGAGTGGTAACCACCAC
  ACGGGTGCAGCATGCCTCGCCAGCCGGCGCCTACGCCCACACGGTGAACCGCAACTGGTA
  CTCGGATGCCGACGTGCCTGAGCTCGCCCGCCAGGAGGGGTGCCAGGACATCGCCACGCA
  GCTCATCTCCAACATGGACATTGATGTGATCCTAGGTGGAGGCCGAAATACATGTTTCCC
  ATGGGGACCCCAGACCCTGAGTACCAGATGACTACAGCCAAGGTGGGACCAGGCTGGACG
  GGAAGAATCTGTGCCAGGAATGGCTGGCAAGCGCCCAGGTGCCC
  
• Restriction Sites: Please inquire
• ACCN: NM_031313
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031313.1, NP_112603.1
• RefSeq Size: 2485 bp
• RefSeq ORF: 1599 bp
• Locus ID: 251
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways
• Gene Summary: 'There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]'