AP1S1 (NM_057089) Human Untagged Clone

CAT#: SC123102

AP1S1 (untagged)-Human adaptor-related protein complex 1, sigma 1 subunit (AP1S1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP1S1
• Synonyms: AP19, CLAPS1, SIGMA1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_057089 edited
  GTGGGACGCGCCGAGCCGGAGGCTGCAGGATGATGCGGTTCATGCTATTATTCAGCCGGC
  AGGGAAAACTGCGGCTGCAAAAATGGTACCTGGCCACTTCGGACAAGGAACGGAAGAAGA
  TGGTGCGCGAGCTCATGCAGGTTGTCCTGGCTCGAAAGCCCAAGATGTGCAGCTTCCTGG
  AGTGGAGGGACCTCAAAGTTGTCTATAAGAGATATGCCAGCCTCTACTTCTGCTGCGCCA
  TCGAGGGCCAAGACAATGAGCTCATCACACTGGAGCTGATCCACCGATACGTGGAGCTCT
  TAGACAAATACTTTGGCAGTGTGTGCGAGCTGGACATCATCTTCAACTTTGAGAAGGCCT
  ACTTCATCCTGGATGAGTTTTTGATGGGGGGGGATGTCCAGGACACCTCCACTTTCCCCT
  TTTCCCACTGAAGGTTTTAGAAGCTAGGAGGCAGGAAAATGTGACCCAGATGGGGGTGCT
  ATTTGGCTTTTATTCCCTGCCTTTGCAGAACTGATGTCACCCCAGATGTCCTTCCCTCCC
  TAATAACTGTAAATATATAAATATGTCAGGTTAAAGGGAAAAGGTGTTCAGGGCACTTCT
  TGTCCTCTCTGTCCCATAACCTACCTCCACCCTCCCCCTAGCCAGCCAGGCAGCTTCTCT
  GCCTGGGAGGGGAGCCTGGACCCCCCTCTTTCTCCTTGGCTGCAGTGGGGCCTTTATCCA
  GTGCCAGGGAGGAACAACATAGTTAATTTTTTTCTAACCTTGCCACTTTGAGGGAAGGAA
  GGGTTGGGGGAAGGGCAAGCTTTATGGGACCCTGGTCCTGGCCCTGGCCTTTCACTCCAG
  TTCTGGGTGAGGCAGGAGCTGGGAGGGGTGGGGAGGGGGAGGGGGAAGTGTCTGCCTTTA
  TGTCCTTTCTTCTGAAATAAAAGGAAAAGCATTTCTGGAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_057089 unedited
  AAAAAACTGCAGGATTTGTAAACGACTTACTATAGGCGGCCGCGNAATTCGCACNGGGTG
  GGACGCGCCGAGCCGGNAGCTGCAGGATGATGCGGTTCATGCTATTATTCAGCCGGCAGG
  GAAAACTGCGGCTGCAAAAATGGTACCTGGCCACTTCGGACAAGGAACGGAAGAAGATGG
  TGCGCGAGCTCATGCAGGTTGTCCTGGCTCGAAAGCCCAAGATGTGCAGCTTCCTGGAGT
  GGAGGGACCTCAAAGTTGTCTATAAGAGATATGCCAGCCTCTACTTCTGCTGCGCCATCG
  AGGGCCAAGACAATGAGCTCATCACACTGGAGCTGATCCACCGATACGTGGAGCTCTTAG
  ACAAATACTTTGGCAGTGTGTGCGAGCTGGACATCATCTTCAACTTTGAGAAGGCCTACT
  TCATCCTGGATGAGTTTTTGATGGGGGNGGGATGTCCAGGACACCTCCACTTTCCCCTTT
  TCCCACTGAAGGTTTTAGAAGCTAGGAGGCAGGAAAATGTGACCCAGATGGGGGTGCTAT
  TTGGCTTTTATTCCCTGCCTTTGCAGAACTGATGTCACCCCAGATGTCCTTCCCTCCCTA
  ATAACTGTAAATATATAAATATGTCAGGTTAAAGGGAAAAGGTGTTCAGGGCACTTCTTG
  TCCTCTCTGTCCCATAACCTACCTCCACCCTCCCCCTAGCCAGCCAGGCAGCTTCTCTGC
  CTGGGAGGGGAGCCTGGACCCCCCTCTTTCTCCTTGGCTGCAGTGGGGCCTTTATCCAGT
  GCCAGGGAGGAACAACATAGTTAATTTTTTTCTAACCTTGCCACTTTGAGGGAAGAAGGG
  TTGNGNGGAAGGGCAAGCTTATGGGACCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_057089
• Insert Size: 958 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057089.1, NP_476430.1
• RefSeq Size: 958 bp
• RefSeq ORF: 402 bp
• Locus ID: 1174
• Cytogenetics: 7q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• Gene Summary: 'The protein encoded by this gene is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains an alternate 3' region including a part of the coding sequence, when compared to variant 1. The encoded protein thus has a shorter, alternate C-terminus, as compared to isoform 1.