PPP2R1B (NM_181699) Human Untagged Clone

CAT#: SC123371

PPP2R1B (untagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2


  "NM_181699" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPP2R1B
Synonyms PP2A-Abeta; PR65B
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_181699 edited
AGGAGCAGGAGGAGAAAGAACATGGCGGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCA
GCGGGTGGAGATGGAGATGATTCGCTATACCCGATCGCGGTTTTAATCGACGAGCTCCGC
AATGAAGACGTGCAGCTCCGACTCAACAGTATTAAGAAGTTATCAACAATTGCCCTAGCA
CTTGGAGTAGAAAGGACCCGAAGTGAATTGTTGCCATTTCTTACAGATACAATTTATGAT
GAAGATGAGGTACTATTAGCTCTTGCTGAGCAGCTGGGAAATTTCACTGGCCTAGTGGGA
GGTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAATCTGGCAACTGTGGAAGAG
ACTGTTGTTCGTGACAAGGCTGTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCT
GTTGCTCTGGAAGCTTATTTTGTACCTCTGGTGAAACGCTTAGCAAGTGGGGATTGGTTC
ACCTCTCGCACATCTGCATGTGGTTTGTTCAGCGTTTGCTATCCCAGGGCATCAAATGCT
GTTAAAGCAGAAATCAGACAGCAATTCCGTTCCTTGTGCTCAGATGACACACCAATGGTA
CGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTAGACAGTGTG
AAAAGTGAAATTGTTCCACTGTTCACTAGTCTAGCTTCAGATGAACAGGATTCAGTGCGC
CTCCTTGCTGTGGAAGCTTGTGTCAGTATTGCCCAGTTATTGTCTCAGGATGACCTTGAG
ACTTTGGTGATGCCTACACTTCGACAAGCAGCAGAAGATAAATCTTGGCGCGTTCGCTAT
ATGGTGGCTGACAGATTTTCAGAGCTCCAGAAAGCCATGGGTCCTAAAATCACCCTAAAT
GACCTCATCCCCGCCTTTCAGAACCTACTTAAAGACTGTGAAGCTGAAGTCCGGGCTGCT
GCTGCCCACAAAGTAAAAGAACTTGGTGAGAACTTGCCCATTGAAGATAGAGAGACCATA
ATTATGAATCAAATTCTGCCTTATATAAAGGAATTAGTATCCGATACCAATCAACATGTC
AAATCGGCTCTAGCTTCTGTAATTATGGGATTGTCTACTATTTTGGGCAAAGAAAATACC
ATTGAACATCTTCTACCTCTTTTCTTAGCTCAGTTAAAGGATGAGTGTCCTGACGTTCGT
TTGAATATCATCTCCAATTTGGATTGTGTAAATGAAGTGATTGGAATCCGTCAGCTCTCT
CAGTCTCTCCTTCCTGCCATAGTGGAGCTGGCAGAAGATGCCAAATGGAGGGTCCGCCTG
GCCATCATTGAGTATATGCCGCTGCTGGCAGGCCAGCTGGGTGTGGAATTCTTTGATGAA
AAGCTGAATTCTTTATGTATGGCTTGGCTCGTGGACCATGTATACGCCATCCGAGAAGCT
GCCACCAACAACCTCATGAAACTAGTTCAGAAGTTTGGTACAGAGTGGGCCCAAAATACT
ATTGTTCCCAAAGTGTTAGTAATGGCAAATGATCCTAATTACTTGCATAGAATGACCACT
TTATTCTGCATTAATGCACTGTCTGAGGCCTGTGGTCAGGAAATAACTACTAAGCAAATG
CTGCCCATCGTATTAAAAATGGCAGGAGACCAAGTAGCAAATGTTCGCTTCAATGTGGCC
AAATCTCTACAAAAGATTGGACCAATTCTAGATACCAATGCTTTACAGGGAGAAGTGAAG
CCAGTACTACAGAAGTTAGGTCAAGATGAAGACATGGATGTCAAATACTTTGCACAGGAA
GCTATAAGTGTGGTGGCACAAAGGCTGAGGAAGCTAGAATTTCCTGTGAAGGACAGTGGA
GAACCCAGTGTCCCTCGGGCTGACAAGAACCACTTCCCAAGACCCACAGTGCCTGGAGAG
GACATGGGGAAGGGACCAGTGTATCAGTTGCGTGGAGATACTAGAGACACACTTGCCCAG
CTGGGAATTGCAGAGCTAGTGCATTTCTCCCAAAGCACAGACTAATACCCCAGATTTAAA
AAAAGAACATAAAAAATAAAGTACCACACATATTACAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_181699 unedited
GGCCGAATACACTTCCTATAGCATACATTATACAATANTTATGGTAGTCAGGCCAAGGGG
GGCGCGCGGCGGGGATTCATAAAAAGCGGAGGAGCAGGAGGAGAAAGAACATGGCGGGCG
CATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTGGAGATGGAGATGATTCGCTATACC
CGATCGCGGTTTTTATTTACGAGCTCCGCAATGAAGACGTGGGGGGGCGACTCAACAGTA
TTAAGAAGTTATCAACAATTGCCCTAGCACTTGGAGTAGAAAGGACCCGAAGTGAATTGT
TGCCATTTCTTACAGATACAATTTATGATGAAGATGAGGTACTATTAGCTCTTGCTGAGC
AGCTGGGAAATTTCACTGGCCTAGTGGGAGGTCCTGACTTTGCCCACTGTCTGCTGCCTC
CTTTGGAAAATCTGGCAACTGTGGAAGAGACTGTTGTTCGTGACAAGGCTGTGGAGTCCC
TGAGACAGATCTCCCAGGAGCATACTCCTGTTGCTCTGGAAGCTTATTTTGTACCTCTGG
TGAAACGCTTAGCAAGTGGGGATTGGTTCACCTCTCGCACATCTGCGTGTGGTTTGGTCA
GCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAAGCAGAAATCAGACAGCAATTCCGTT
CCTTGTGCTCAGATGACACACCAATGGTACGACGTGCTGCTGCTTCCAAATTGGGTGAAT
TGCAAAAGTGTTGGAATTAGACAGTGTGAAAAGGGAATTGTTGCCCTGTTCGCTAGTCTA
GCTCCAGATGAACGGGATTCAGTGCGCCTCCTTGGTGGGAGAACTTGGGTCATTATTGCC
CAATTTTGGCTCAGGGGAACCTTGAAACTTGGGGGAGCGCACCTTCCAACAGCGGGN
Restriction Sites Please inquire     
ACCN NM_181699
Insert Size 2097 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_181699.1, NP_859050.1
RefSeq Size 2139 bp
RefSeq ORF 2004 bp
Locus ID 5519
Cytogenetics 11q23.1
Protein Families Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
Gene Summary 'This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'
Transcript Variant: This variant (2) differs in the 3' UTR and the 3' coding region, compared to variant 1. The resulting isoform (b) contains a longer and distinct C-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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