CDK6 (BC027989) Human Untagged Clone

CAT#: SC123532

(untagged)-Homo sapiens, Similar to LOC168246, clone MGC:40162 IMAGE:4995539, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK6
• Synonyms: MCPH12|PLSTIRE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC027989 edited
  CCACGCGTCCGCTTTCTCTTAGGTTTCAGGAAGACTGTCACATACAGTAAGCTTTTTTCC
  TTCTGACTTATAATAGAAAATGTTTTGAAAGTAAAAAAAAAAAATCTAATTTGGAAATTT
  GACTTGTTAGTTTCTGTGTTTGAAATCATGGTTCTAGAAATGTAGAAATTGTGTATATCA
  GATACTCATCTAGGCTGTGTGAACCAGCCCAAGATGACCAACATCCCCACACCTCTACAT
  CTCTGTCCCCTGTATCTCTTCCTTTCTACCACTAAAGTGTTCCCTGCTACCATCCTGGCT
  TGTCCACATGGTGCTCTCCATCTTCCTCCACATCATGGACCACAGGTGTGCCTGTCTAGG
  CCTGGCCACCACTCCCAACTTGACCTAGCCACATTCATCTAGAGATGGTTCCTGATGCTG
  GGCACAGACTGTGCTCATGGCACCCATTAGAAATGCCTCTAGCATCTTTGTATGCATCTT
  GATTTTTAAACCAAGTCATTGTACAGAGCATTCAGTTTTGGCTGTGGTACCAAGAGAAAA
  ACTAATCAAGAATATAAACCACATTCCAGGCTGCTGTTTTCTCTCCATCTACAGGCCACA
  CTTTTACTGTATTTCTTCATACTTGAAATTCATTCTGCTATTTTCATATCAGGGTACAGA
  CTTATAAGGGTGCATGTTCCTTAAAGGTGCATAATTATTCTTATTCCGTTTGCTTATATT
  GCTACAGAATGCTCTGTTTTGGTGCTTTGAGTTCTGCAGACCCAAGAAGCAGTGTGGAAA
  TTCACTGCCTGGGACACAGTCTTATAAGAATGTTGGCAGGTGACTTTGTATCAGATGTTG
  CTTCTCTTTTCTCTGTACACAGATTGAGAGTTACCACAGTGGCCTGTCGGGTCCACCCTG
  TGGGTGCAGCACAGCTCTCTGAAAGCAAGAACCTTCCTACCTATTCTAACGTTTTTGCCC
  TCTAAGAAAAATGGCCTCAGGTATGGTATAGACATAGCAAGAGGGGAAGGGCTGTCTCAC
  TCTAGCAACCATCCCTCCATTACACACAGAAAGCCCTCTTGAAGCAAAAGAAGAAGAAAG
  AAAGAAAGCTTATCTCTAAGGCTACTGTCTTCAGAATGCTCTGAGCTGAATGCTCTTGCT
  CCTTTCCCAAGAGGCAGATGAAAATATAGCCAGTTTATCTATACCCTTCCTATCTGAGGA
  GGAGAATAGAAAAGTAGGGTAAATATGTAACGTAAAATATGTCATTCAAGGACCACCAAA
  ACTTTAAGTACCCTATCATTAAAAATCTGGTTTTAAAAGTAGCTCAAGTAAGGGATGCTT
  TGTGACCCAGGGTTTCTGAAGTCAGATAGCCATTCTTACCTGCCCCTTACTCTGACTTAT
  TGGGAAAGGGAGAACTGCAGTGGTGTTTCTGTTGCAGTGGCAAAGGTAACATGTCAGAAA
  ATTCAGAGGGTTGCATACCAATAATCCTTTGGAAACTGGATGTCTTACTGGGTGCTAGAA
  TGAAAATGTAGGTATTTATTGTCAGATGATGAAGTTCATTGTTTTTTTCAAAATTGGTGT
  TGAAATATCACTGTCCAATGTGTTCACTTATGTGAAAGCTAAATTGAATGAGGCAAAAAG
  AGCAAATAGTTTGTATATTTGTAATACCTTTTGTATTTCTTACAATAAAAATATTGGTAG
  CAAATAAAAATAATAAAAACAATAACTTTAAACTGCAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC027989 unedited
  GGGTTANGATTTGTATACGACTCACTATAGGGCGGCCGCGATTCCCGGGATATCGTCGAC
  CCACGCGTCCGCTTTCTCTTAGGTTTCAGGAAGACTGTCACATACAGTAAGCTTTTTTCC
  TTCTGACTTATAATAGAAAATGTTTTGAAAGTAAAAAAAAAAAATCTAATTTGGAAATTT
  GACTTGTTAGTTTCTGTGTTTGAAATCATGGTTCTAGAAATGTAGAAATTGTGTATATCA
  GATACTCATCTAGGCTGTGTGAACCAGCCCAAGATGACCAACATCCCCACACCTCTACAT
  CTCTGTCCCCTGTATCTCTTCCTTTCTACCACTAAAGTGTTCCCTGCTACCATCCTGGCT
  TGTCCACATGGTGCTCTCCATCTTCCTCCACATCATGGACCACAGGTGTGCCTGTCTAGG
  CCTGGCCACCACTCCCAACTTGACCTAGCCACATTCATCTAGAGATGGTTCCTGATGCTG
  GGCACAGACTGTGCTCATGGCACCCATTAGAAATGCCTCTAGCATCTTTGTATGCATCTT
  GATTTTTAAACCAAGTCATTGTACAGAGCATTCAGTTTTGGCTGTGGTACCAAGAGAAAA
  ACTAATCAAGAATATAAACCACATTCCAGGCTGCTGTTTTCTCTCCATCTACAGGCCACA
  CTTTTACTGTATTTCTTCATACTTGAAATTCATTCTGCTATTTTCATATCAGGGTACAGA
  CTTATAAGGGTGCATGTTCCTNTAAGGTGCATAATTATTCTTATTCCGTTTGCTTATATT
  GCTACAGAATGCTCTGTTTTGGTGCTTTGAGTTCTGCAGACCCAAGAAGCAGTGTGGGAA
  TTCACTGCCTGGGGACACAGTCTATAAGAATGTNGGCAGGTGACTTTGTATCAGATGTGG
  CTTCTCTTTTTCTGN
  
• Restriction Sites: Please inquire
• ACCN: BC027989
• Insert Size: 1748 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027989.1
• RefSeq Size: 1748 bp
• Locus ID: 1021
• Cytogenetics: 7q21.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]'