Trefoil Factor 3 (TFF3) (BC017859) Human Untagged Clone

CAT#: SC123595

TFF3 (untagged)-Human trefoil factor 3 (intestinal) (cDNA clone MGC:22588 IMAGE:4696566), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFF3
• Synonyms: HITF; hP1.B; intestinal trefoil factor; ITF; secretory protein; TFI; trefoil factor 3; trefoil factor 3 (intestinal); trefoil factor 3, HITF, human intestinal trefoil factor
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC017859 edited
  GGAGTCCTGAGCTGCGTCCCGGAGCCCACGGTGGTCATGGCTGCCAGAGCGCTCTGCATG
  CTGGGGCTGGTCCTGGCCTTGCTGTCCTCCAGCTCTGCTGAGGAGTACGTGGGCCTGTCT
  GCAAACCAGTGTGCCGTGCCAGCCAAGGACAGGGTGGACTGCGGCTACCCCCATGTCACC
  CCCAAGGAGTGCAACAACCGGGGCTGCTGCTTTGACTCCAGGATCCCTGGAGTGCCTTGG
  TGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGCTGCCCCCGG
  CCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCAGGCACTGTTC
  ATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTTCATATCTGG
  AGCCTGATGTCTTAACGAATAAAGGTCCCATGCTCCACCCTAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC017859 unedited
  NNNNGGTTCCGTCATTATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCCATTA
  CGGCCGGGGGGAGTCCTGAGCTGCGTCCCGGAGCCCACGGTGGTCATGGCTGCCAGAGCG
  CTCTGCATGCTGGGGCTGGTCCTGGCCTTGCTGTCCTCCAGCTCTGCTGAGGAGTACGTG
  GGCCTGTCTGCAAACCAGTGTGCCGTGCCAGCCAAGGACAGGGTGGACTGCGGCTACCCC
  CATGTCACCCCCAAGGAGTGCAACAACCGGGGCTGCTGCTTTGACTCCAGGATCCCTGGA
  GTGCCTTGGTGTTTCAAGCCCCTGCAGGAAGCAGAATGCACCTTCTGAGGCACCTCCAGC
  TGCCCCCGGCCGGGGGATGCGAGGCTCGGAGCACCCTTGCCCGGCTGTGATTGCTGCCAG
  GCACTGTTCATCTCAGCTTTTCTGTCCCTTTGCTCCCGGCAAGCGCTTCTGCTGAAAGTT
  CATATCTGGAGCCTGATGTCTTAACGAATAAAGGTCCCATGCTCCACCCTAAAAAAAAAA
  AAAAAAAAANNNNNNNNNCCTTGTCGGCCGCCTCGGCCCTCGAGTTNNNNNNNNNNNNNN
  TTTTTTTTTTTTCCGCGTTCATTATCTCATTTAATTCTTACAAAACCACGGCAAGGATTC
  AACCCCACAACTGCTGTCTAGAAAACCGAGGAAAGTGACCCACCCCGCGGGAGGTCTGAC
  CGCCCGAGAGCCCCACCCCCAGGCCCGGGAACCACCCCCGTCCAATTTCCCGGGGGATTC
  TTTTTCGTTTCCCCGCGGCCCGCCCAGGTGAACCTCCCTTCCCCCCTACAAAAGGAGCTC
  CCAACGGGGAACCACTTCGAGGGCAACCCCGGGGCCCCCCGGGAG
  
• Restriction Sites: Please inquire
• ACCN: BC017859
• Insert Size: 491 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017859.1, AAH17859.1
• RefSeq Size: 491 bp
• Locus ID: 7033
• Cytogenetics: 21q22.3
• Protein Families: Secreted Protein
• Gene Summary: 'Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]'